Noggin (NOG) (NM_005450) Human Tagged ORF Clone
NOG (Myc-DDK-tagged)-Human noggin (NOG)
|Product Name||Noggin (NOG) (NM_005450) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||SYM1; SYNS1; SYNS1A|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC205020 representing NM_005450
Red=Cloning site Blue=ORF Green=Tags(s)
>RC205020 representing NM_005450
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-RsrII Cloning Scheme for this gene Plasmid Map|
|ORF Size||696 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_005450.1, NM_005450.2, NM_005450.3, NM_005450.4, NP_005441.1|
|Protein Families||Druggable Genome, Secreted Protein|
|Protein Pathways||TGF-beta signaling pathway|
|Gene Summary||The secreted polypeptide, encoded by this gene, binds and inactivates members of the transforming growth factor-beta (TGF-beta) superfamily signaling proteins, such as bone morphogenetic protein-4 (BMP4). By diffusing through extracellular matrices more efficiently than members of the TGF-beta superfamily, this protein may have a principal role in creating morphogenic gradients. The protein appears to have pleiotropic effect, both early in development as well as in later stages. It was originally isolated from Xenopus based on its ability to restore normal dorsal-ventral body axis in embryos that had been artificially ventralized by UV treatment. The results of the mouse knockout of the ortholog suggest that it is involved in numerous developmental processes, such as neural tube fusion and joint formation. Recently, several dominant human NOG mutations in unrelated families with proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) were identified; both SYM1 and SYNS1 have multiple joint fusion as their principal feature, and map to the same region (17q22) as this gene. All of these mutations altered evolutionarily conserved amino acid residues. The amino acid sequence of this human gene is highly homologous to that of Xenopus, rat and mouse. [provided by RefSeq, Jul 2008]|
|The use of this cDNA Clones has been cited in the following citations:|
Organoid cultures derived from patients with advanced prostate cancer
,Gao, D;Vela, I;Sboner, A;Iaquinta, PJ;Karthaus, WR;Gopalan, A;Dowling, C;Wanjala, JN;Undvall, EA;Arora, VK;Wongvipat, J;Kossai, M;Ramazanoglu, S;Barboza, LP;Di, W;Cao, Z;Zhang, QF;Sirota, I;Ran, L;MacDonald, TY;Beltran, H;Mosquera, JM;Touijer, KA;Scardino,
Cell Sep 2014
,PubMed ID 25201530
|cDNA Clone Resources|
|SC122718||NOG (untagged)-Human noggin (NOG)||
|RG205020||NOG (GFP-tagged) - Human noggin (NOG)||
|RC205020L1||Lenti ORF clone of Human noggin (NOG), Myc-DDK-tagged||
|RC205020L2||Lenti ORF clone of Human noggin (NOG), mGFP tagged||
|RC205020L3||Lenti ORF clone of Human noggin (NOG), Myc-DDK-tagged||
|RC205020L4||Lenti ORF clone of Human noggin (NOG), mGFP tagged||