USF1 (NM_007122) Human Tagged ORF Clone
Lenti ORF clone of Human upstream transcription factor 1 (USF1), transcript variant 1, mGFP tagged
|Type||Human Tagged ORF Clone|
|Synonyms||bHLHb11; FCHL; FCHL1; HYPLIP1; MLTF; MLTFI; UEF|
|E. coli Selection||Chloramphenicol (34 ug/mL)|
|Mammalian Cell Selection||None|
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene|
|ORF Size||930 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||1830 bp|
|RefSeq ORF||933 bp|
|Protein Families||Druggable Genome, Transcription Factors|
|Gene Summary||This gene encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. This gene has been linked to familial combined hyperlipidemia (FCHL). Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been defined on chromosome 21. [provided by RefSeq, Feb 2013]|
|cDNA Clone Resources|