ZNF289 (ARFGAP2) (NM_032389) Human Tagged ORF Clone

CAT#: RC204908

ARFGAP2 (Myc-DDK-tagged)-Human ADP-ribosylation factor GTPase activating protein 2 (ARFGAP2)

Product Images

Western blot validation of overexpression lysate (Cat# LY410154) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204908 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARFGAP2
• Synonyms: IRZ; NBLA10535; ZFP289; ZNF289
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204908 representing NM_032389
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGAGCCGAACAAGACCGAAATCCAGACTCTTTTTAAGAGGCTTCGCGCAGTTCCAACCAACA
  AGGCCTGTTTCGACTGCGGCGCCAAGAATCCGAGTTGGGCCAGCATCACGTACGGTGTTTTCTTGTGCAT
  TGACTGTTCCGGGGTGCACCGCTCCCTGGGCGTCCATCTGAGCTTCATCAGGTCCACAGAGTTGGATTCC
  AACTGGAACTGGTTCCAGCTGAGGTGTATGCAGGTCGGCGGGAATGCCAATGCGACGGCTTTTTTTCGCC
  AACATGGATGCACAGCCAATGATGCCAACACCAAATATAATAGCCGAGCTGCCCAGATGTACCGGGAGAA
  GATCCGGCAGCTGGGGAGTGCGGCCCTGGCTAGGCATGGCACTGATCTTTGGATAGACAACATGAGTAGT
  GCCGTTCCTAATCACTCCCCAGAGAAGAAGGACTCTGATTTCTTCACAGAACACACTCAACCCCCTGCCT
  GGGATGCGCCAGCCACTGAGCCTTCAGGGACCCAGCAGCCAGCCCCGTCTACAGAGAGCAGTGGCCTGGC
  ACAGCCGGAGCATGGCCCCAACACAGACCTGCTTGGCACCTCACCCAAAGCCTCACTGGAACTGAAAAGC
  TCCATCATTGGCAAGAAGAAGCCAGCAGCAGCTAAGAAAGGGCTGGGTGCCAAGAAAGGCCTAGGGGCCC
  AGAAGGTGAGCAGCCAGAGCTTCAGTGAGATTGAGCGGCAGGCTCAGGTGGCAGAGAAGCTCCGTGAGCA
  GCAGGCAGCCGATGCCAAGAAGCAGGCGGAGGAGTCCATGGTCGCCTCCATGCGTCTGGCCTACCAGGAG
  CTCCAGATTGATCGTAAGAAAGAGGAAAAGAAGCTACAGAATCTGGAAGGGAAGAAGCGAGAGCAGGCAG
  AAAGGTTGGGCATGGGCTTGGTATCCCGAAGCTCTGTCTCCCACTCCGTGCTGTCTGAGATGCAGGTGAT
  TGAGCAGGAAACCCCAGTGAGTGCAAAATCCTCTCGCTCGCAGCTGGACTTGTTTGACGATGTTGGTACT
  TTCGCCTCTGGACCCCCAAAGTACAAGGACAATCCCTTTTCCTTAGGGGAAAGCTTTGGCTCCCGCTGGG
  ATACAGATGCTGCCTGGGGTATGGACAGGGTAGAGGAGAAGGAGCCAGAAGTGACCATCTCAAGCATCCG
  GCCTATTTCAGAAAGAGCCACAAACCGGAGGGAAGTGGAGAGCCGGAGCTCAGGCCTCGAGTCTAGTGAG
  GCGCGTCAGAAATTCGCAGGAGCCAAAGCCATCTCATCTGACATGTTCTTTGGGCGGGAGGTGGATGCGG
  AGTATGAGGCCAGGTCTCGGCTGCAGCAGCTCTCAGGCAGCAGTGCCATCAGCTCTTCAGACCTCTTTGG
  GGACATGGATGGAGCTCACGGAGCAGGAAGTGTATCTCTGGGGAACGTGCTGCCTACAGCGGACATTGCC
  CAGTTTAAGCAGGGTGTCAAGTCTGTGGCTGGGAAAATGGCTGTGCTGGCCAATGGTGTGATGAATTCCT
  TGCAGGATCGCTACGGTTCCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204908 representing NM_032389
  Red=Cloning site Green=Tags(s)
  
  MAAEPNKTEIQTLFKRLRAVPTNKACFDCGAKNPSWASITYGVFLCIDCSGVHRSLGVHLSFIRSTELDS
  NWNWFQLRCMQVGGNANATAFFRQHGCTANDANTKYNSRAAQMYREKIRQLGSAALARHGTDLWIDNMSS
  AVPNHSPEKKDSDFFTEHTQPPAWDAPATEPSGTQQPAPSTESSGLAQPEHGPNTDLLGTSPKASLELKS
  SIIGKKKPAAAKKGLGAKKGLGAQKVSSQSFSEIERQAQVAEKLREQQAADAKKQAEESMVASMRLAYQE
  LQIDRKKEEKKLQNLEGKKREQAERLGMGLVSRSSVSHSVLSEMQVIEQETPVSAKSSRSQLDLFDDVGT
  FASGPPKYKDNPFSLGESFGSRWDTDAAWGMDRVEEKEPEVTISSIRPISERATNRREVESRSSGLESSE
  ARQKFAGAKAISSDMFFGREVDAEYEARSRLQQLSGSSAISSSDLFGDMDGAHGAGSVSLGNVLPTADIA
  QFKQGVKSVAGKMAVLANGVMNSLQDRYGSY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032389
• ORF Size: 1563 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032389.5
• RefSeq Size: 3039 bp
• RefSeq ORF: 1566 bp
• Locus ID: 84364
• UniProt ID: Q8N6H7
• Cytogenetics: 11p11.2
• Domains: ArfGap
• Protein Pathways: Endocytosis
• MW: 57.2 kDa
• Gene Summary: GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Implicated in coatomer-mediated protein transport between the Golgi complex and the endoplasmic reticulum. Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.[UniProtKB/Swiss-Prot Function]