MTF1 (NM_005955) Human Tagged ORF Clone

CAT#: RC204861

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MTF1 (Myc-DDK-tagged)-Human metal-regulatory transcription factor 1 (MTF1)



  "NM_005955" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol MTF1
Synonyms MTF-1; ZRF
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC204861 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGAACACAGTCCAGACAACAACATCATCTACTTTGAGGCAGAGGAAGATGAGCTGACCCCCGATG
ATAAAATGCTCAGGTTTGTGGATAAAAACGGACTGGTGCCTTCCTCATCTGGAACTGTTTATGATAGGAC
CACTGTTCTTATTGAGCAGGACCCTGGCACTTTGGAGGATGAAGATGACGACGGACAGTGCGGAGAACAC
TTGCCTTTTCTAGTAGGGGGTGAAGAGGGCTTTCACCTGATAGATCATGAAGCAATGTCCCAGGGTTATG
TGCAGCACATTATCTCACCAGATCAGATTCATTTGACAATAAACCCTGGTTCCACACCCATGCCAAGAAA
TATTGAAGGTGCAACCCTCACTCTGCAGTCGGAATGTCCGGAAACAAAACGTAAAGAAGTAAAGCGGTAC
CAATGTACCTTTGAGGGCTGTCCCCGCACCTACAGCACAGCAGGCAACCTGCGAACCCACCAGAAGACTC
ACCGAGGAGAGTACACCTTTGTCTGTAATCAGGAGGGCTGTGGCAAAGCCTTCCTTACCTCTTACAGCCT
CAGGATCCACGTGCGAGTGCACACGAAGGAGAAGCCATTTGAGTGTGACGTGCAGGGCTGTGAGAAGGCA
TTCAACACACTGTACAGGCTGAAAGCACATCAGAGGCTTCACACAGGGAAAACGTTTAACTGTGAATCTG
AAGGCTGCAGCAAATACTTCACCACACTCAGTGATCTGAGGAAGCACATTCGAACTCATACAGGGGAAAA
GCCATTTCGGTGCGATCACGATGGCTGTGGAAAAGCATTTGCAGCAAGCCACCACCTTAAAACTCACGTT
CGTACACATACTGGTGAAAGACCCTTCTTCTGCCCCAGTAATGGCTGTGAGAAAACATTCAGCACTCAAT
ACAGTCTCAAAAGTCACATGAAAGGTCATGATAACAAAGGACACTCATACAATGCACTTCCACAACACAA
TGGATCAGAGGATACAAATCACTCACTTTGTCTAAGTGACTTGAGCCTTCTGTCCACAGATTCTGAATTG
CGAGAAAATTCCAGTACGACCCAGGGCCAGGACCTCAGCACAATTTCACCAGCAATCATCTTTGAATCAA
TGTTCCAGAATTCAGATGATACGGCAATTCAGGAAGATCCTCAACAGACGGCTTCCTTGACTGAAAGTTT
TAATGGTGATGCAGAGTCAGTCAGTGATGTTCCGCCATCCACAGGAAATTCAGCATCTTTATCTCTTCCA
CTTGTACTGCAACCTGGCCTCTCCGAGCCACCCCAGCCTCTACTACCTGCCTCAGCTCCGTCTGCTCCTC
CGCCTGCTCCCTCCCTAGGACCTGGCTCCCAGCAAGCTGCATTTGGCAACCCCCCTGCTCTCTTACAACC
TCCAGAAGTGCCTGTTCCCCACAGCACACAGTTTGCTGCTAATCATCAAGAGTTTCTTCCGCACCCCCAG
GCACCGCAGCCCATTGTACCAGGACTTTCTGTTGTTGCTGGGGCTTCTGCATCAGCAGCGGCAGTGGCAT
CAGCTGTGGCAGCACCAGCCCCACCACAAAGTACTACTGAGCCCCTGCCAGCCATGGTCCAGACTCTGCC
CCTGGGTGCCAACTCTGTCCTAACTAATAATCCCACAATAACCATCACCCCAACTCCCAACACAGCTATC
CTGCAGTCCAGCCTAGTCATGGGAGAACAGAACTTACAATGGATATTAAATGGTGCCACCAGTTCTCCAC
AAAACCAAGAACAAATTCAGCAAGCATCTAAAGTTGAGAAGGTGTTTTTTACCACTGCAGTACCAGTAGC
CAGTAGCCCAGGGAGCTCTGTCCAGCAGATTGGCCTCAGTGTTCCTGTGATCATCATCAAACAAGAAGAG
GCATGTCAGTGTCAGTGTGCATGCCGGGACTCTGCAAAGGAGCGGGCATCCAGCAGGAGAAAGGGCTGCT
CCTCCCCACCCCCTCCAGAGCCGAGCCCCCAGGCTCCTGATGGGCCCAGCCTGCAGCTCCCAGCGCAGAC
TTTCTCTTCAGCCCCTGTTCCCGGGTCATCATCCTCTACCTTGCCCTCCTCCTGTGAGCAAAGCCGACAA
GCAGAGACTCCTTCAGACCCTCAGACAGAAACATTAAGTGCCATGGATGTGTCAGAGTTTCTATCCCTCC
AGAGCCTGGACACCCCGTCCAATCTGATTCCCATTGAAGCACTACTGCAGGGGGAGGAGGAGATGGGCCT
CACCAGCAGCTTCTCCAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC204861 protein sequence
Red=Cloning site Green=Tags(s)

MGEHSPDNNIIYFEAEEDELTPDDKMLRFVDKNGLVPSSSGTVYDRTTVLIEQDPGTLEDEDDDGQCGEH
LPFLVGGEEGFHLIDHEAMSQGYVQHIISPDQIHLTINPGSTPMPRNIEGATLTLQSECPETKRKEVKRY
QCTFEGCPRTYSTAGNLRTHQKTHRGEYTFVCNQEGCGKAFLTSYSLRIHVRVHTKEKPFECDVQGCEKA
FNTLYRLKAHQRLHTGKTFNCESEGCSKYFTTLSDLRKHIRTHTGEKPFRCDHDGCGKAFAASHHLKTHV
RTHTGERPFFCPSNGCEKTFSTQYSLKSHMKGHDNKGHSYNALPQHNGSEDTNHSLCLSDLSLLSTDSEL
RENSSTTQGQDLSTISPAIIFESMFQNSDDTAIQEDPQQTASLTESFNGDAESVSDVPPSTGNSASLSLP
LVLQPGLSEPPQPLLPASAPSAPPPAPSLGPGSQQAAFGNPPALLQPPEVPVPHSTQFAANHQEFLPHPQ
APQPIVPGLSVVAGASASAAAVASAVAAPAPPQSTTEPLPAMVQTLPLGANSVLTNNPTITITPTPNTAI
LQSSLVMGEQNLQWILNGATSSPQNQEQIQQASKVEKVFFTTAVPVASSPGSSVQQIGLSVPVIIIKQEE
ACQCQCACRDSAKERASSRRKGCSSPPPPEPSPQAPDGPSLQLPAQTFSSAPVPGSSSSTLPSSCEQSRQ
AETPSDPQTETLSAMDVSEFLSLQSLDTPSNLIPIEALLQGEEEMGLTSSFSK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_005955
ORF Size 2259 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005955.3
RefSeq Size 7986 bp
RefSeq ORF 2262 bp
Locus ID 4520
UniProt ID Q14872
Cytogenetics 1p34.3
Domains zf-C2H2
Protein Families Transcription Factors
MW 81 kDa
Gene Summary This gene encodes a transcription factor that induces expression of metallothioneins and other genes involved in metal homeostasis in response to heavy metals such as cadmium, zinc, copper, and silver. The protein is a nucleocytoplasmic shuttling protein that accumulates in the nucleus upon heavy metal exposure and binds to promoters containing a metal-responsive element (MRE). [provided by RefSeq, Jul 2008]

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