MTF1 (NM_005955) Human Tagged ORF Clone

CAT#: RC204861

MTF1 (Myc-DDK-tagged)-Human metal-regulatory transcription factor 1 (MTF1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MTF1 (Cat# RC204861, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MTF1 (Cat# TA805480)(1:2000). Positive lysates LY401804 (100ug) and LC401804 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY401804) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204861 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MTF1 protein (Cat# TP304861). The protein was produced from HEK293T cells transfected with MTF1 cDNA clone (Cat# RC204861) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MTF1
• Synonyms: MTF-1; ZRF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204861 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGAACACAGTCCAGACAACAACATCATCTACTTTGAGGCAGAGGAAGATGAGCTGACCCCCGATG
  ATAAAATGCTCAGGTTTGTGGATAAAAACGGACTGGTGCCTTCCTCATCTGGAACTGTTTATGATAGGAC
  CACTGTTCTTATTGAGCAGGACCCTGGCACTTTGGAGGATGAAGATGACGACGGACAGTGCGGAGAACAC
  TTGCCTTTTCTAGTAGGGGGTGAAGAGGGCTTTCACCTGATAGATCATGAAGCAATGTCCCAGGGTTATG
  TGCAGCACATTATCTCACCAGATCAGATTCATTTGACAATAAACCCTGGTTCCACACCCATGCCAAGAAA
  TATTGAAGGTGCAACCCTCACTCTGCAGTCGGAATGTCCGGAAACAAAACGTAAAGAAGTAAAGCGGTAC
  CAATGTACCTTTGAGGGCTGTCCCCGCACCTACAGCACAGCAGGCAACCTGCGAACCCACCAGAAGACTC
  ACCGAGGAGAGTACACCTTTGTCTGTAATCAGGAGGGCTGTGGCAAAGCCTTCCTTACCTCTTACAGCCT
  CAGGATCCACGTGCGAGTGCACACGAAGGAGAAGCCATTTGAGTGTGACGTGCAGGGCTGTGAGAAGGCA
  TTCAACACACTGTACAGGCTGAAAGCACATCAGAGGCTTCACACAGGGAAAACGTTTAACTGTGAATCTG
  AAGGCTGCAGCAAATACTTCACCACACTCAGTGATCTGAGGAAGCACATTCGAACTCATACAGGGGAAAA
  GCCATTTCGGTGCGATCACGATGGCTGTGGAAAAGCATTTGCAGCAAGCCACCACCTTAAAACTCACGTT
  CGTACACATACTGGTGAAAGACCCTTCTTCTGCCCCAGTAATGGCTGTGAGAAAACATTCAGCACTCAAT
  ACAGTCTCAAAAGTCACATGAAAGGTCATGATAACAAAGGACACTCATACAATGCACTTCCACAACACAA
  TGGATCAGAGGATACAAATCACTCACTTTGTCTAAGTGACTTGAGCCTTCTGTCCACAGATTCTGAATTG
  CGAGAAAATTCCAGTACGACCCAGGGCCAGGACCTCAGCACAATTTCACCAGCAATCATCTTTGAATCAA
  TGTTCCAGAATTCAGATGATACGGCAATTCAGGAAGATCCTCAACAGACGGCTTCCTTGACTGAAAGTTT
  TAATGGTGATGCAGAGTCAGTCAGTGATGTTCCGCCATCCACAGGAAATTCAGCATCTTTATCTCTTCCA
  CTTGTACTGCAACCTGGCCTCTCCGAGCCACCCCAGCCTCTACTACCTGCCTCAGCTCCGTCTGCTCCTC
  CGCCTGCTCCCTCCCTAGGACCTGGCTCCCAGCAAGCTGCATTTGGCAACCCCCCTGCTCTCTTACAACC
  TCCAGAAGTGCCTGTTCCCCACAGCACACAGTTTGCTGCTAATCATCAAGAGTTTCTTCCGCACCCCCAG
  GCACCGCAGCCCATTGTACCAGGACTTTCTGTTGTTGCTGGGGCTTCTGCATCAGCAGCGGCAGTGGCAT
  CAGCTGTGGCAGCACCAGCCCCACCACAAAGTACTACTGAGCCCCTGCCAGCCATGGTCCAGACTCTGCC
  CCTGGGTGCCAACTCTGTCCTAACTAATAATCCCACAATAACCATCACCCCAACTCCCAACACAGCTATC
  CTGCAGTCCAGCCTAGTCATGGGAGAACAGAACTTACAATGGATATTAAATGGTGCCACCAGTTCTCCAC
  AAAACCAAGAACAAATTCAGCAAGCATCTAAAGTTGAGAAGGTGTTTTTTACCACTGCAGTACCAGTAGC
  CAGTAGCCCAGGGAGCTCTGTCCAGCAGATTGGCCTCAGTGTTCCTGTGATCATCATCAAACAAGAAGAG
  GCATGTCAGTGTCAGTGTGCATGCCGGGACTCTGCAAAGGAGCGGGCATCCAGCAGGAGAAAGGGCTGCT
  CCTCCCCACCCCCTCCAGAGCCGAGCCCCCAGGCTCCTGATGGGCCCAGCCTGCAGCTCCCAGCGCAGAC
  TTTCTCTTCAGCCCCTGTTCCCGGGTCATCATCCTCTACCTTGCCCTCCTCCTGTGAGCAAAGCCGACAA
  GCAGAGACTCCTTCAGACCCTCAGACAGAAACATTAAGTGCCATGGATGTGTCAGAGTTTCTATCCCTCC
  AGAGCCTGGACACCCCGTCCAATCTGATTCCCATTGAAGCACTACTGCAGGGGGAGGAGGAGATGGGCCT
  CACCAGCAGCTTCTCCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204861 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGEHSPDNNIIYFEAEEDELTPDDKMLRFVDKNGLVPSSSGTVYDRTTVLIEQDPGTLEDEDDDGQCGEH
  LPFLVGGEEGFHLIDHEAMSQGYVQHIISPDQIHLTINPGSTPMPRNIEGATLTLQSECPETKRKEVKRY
  QCTFEGCPRTYSTAGNLRTHQKTHRGEYTFVCNQEGCGKAFLTSYSLRIHVRVHTKEKPFECDVQGCEKA
  FNTLYRLKAHQRLHTGKTFNCESEGCSKYFTTLSDLRKHIRTHTGEKPFRCDHDGCGKAFAASHHLKTHV
  RTHTGERPFFCPSNGCEKTFSTQYSLKSHMKGHDNKGHSYNALPQHNGSEDTNHSLCLSDLSLLSTDSEL
  RENSSTTQGQDLSTISPAIIFESMFQNSDDTAIQEDPQQTASLTESFNGDAESVSDVPPSTGNSASLSLP
  LVLQPGLSEPPQPLLPASAPSAPPPAPSLGPGSQQAAFGNPPALLQPPEVPVPHSTQFAANHQEFLPHPQ
  APQPIVPGLSVVAGASASAAAVASAVAAPAPPQSTTEPLPAMVQTLPLGANSVLTNNPTITITPTPNTAI
  LQSSLVMGEQNLQWILNGATSSPQNQEQIQQASKVEKVFFTTAVPVASSPGSSVQQIGLSVPVIIIKQEE
  ACQCQCACRDSAKERASSRRKGCSSPPPPEPSPQAPDGPSLQLPAQTFSSAPVPGSSSSTLPSSCEQSRQ
  AETPSDPQTETLSAMDVSEFLSLQSLDTPSNLIPIEALLQGEEEMGLTSSFSK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005955
• ORF Size: 2259 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005955.3
• RefSeq Size: 7986 bp
• RefSeq ORF: 2262 bp
• Locus ID: 4520
• UniProt ID: Q14872
• Cytogenetics: 1p34.3
• Domains: zf-C2H2
• Protein Families: Transcription Factors
• MW: 81 kDa
• Gene Summary: This gene encodes a transcription factor that induces expression of metallothioneins and other genes involved in metal homeostasis in response to heavy metals such as cadmium, zinc, copper, and silver. The protein is a nucleocytoplasmic shuttling protein that accumulates in the nucleus upon heavy metal exposure and binds to promoters containing a metal-responsive element (MRE). [provided by RefSeq, Jul 2008]