YARS2 (NM_001040436) Human Tagged ORF Clone

CAT#: RC204828

YARS2 (Myc-DDK-tagged)-Human tyrosyl-tRNA synthetase 2, mitochondrial (YARS2), nuclear gene encoding mitochondrial protein

Product Images

Circular map for RC204828

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  Western blot validation of overexpression lysate (Cat# LY421754) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204828 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified YARS2 protein (Cat# TP304828). The protein was produced from HEK293T cells transfected with YARS2 cDNA clone (Cat# RC204828) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: YARS2
• Synonyms: CGI-04; MLASA2; MT-TYRRS; TYRRS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204828 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGCCCATCTTGCGGTCCTTTTCCTGGGGCCGGTGGTCTGGTACCCTAAATCTCTCAGTATTGT
  TGCCCTTGGGGCTGCGTAAGGCCCACTCGGGCGCTCAGGGGTTACTGGCAGCGCAGAAGGCTCGAGGTCT
  GTTCAAGGACTTCTTCCCGGAGACGGGGACGAAAATAGAGCTCCCAGAGCTCTTCGACCGTGGCACGGCG
  AGTTTTCCCCAAACCATTTACTGTGGCTTCGACCCCACGGCAGACTCGCTTCATGTGGGTCATCTACTTG
  CGCTGCTGGGCCTGTTTCATTTGCAGCGAGCGGGCCACAACGTGATCGCGCTGGTGGGAGGCGCCACGGC
  GCGCCTGGGAGACCCGAGCGGCCGTACCAAGGAACGCGAGGCGCTGGAGACAGAGCGCGTGCGAGCCAAC
  GCGCGAGCTCTGCGCCTAGGGCTTGAGGCCCTGGCGGCTAATCACCAGCAGCTTTTCACTGATGGGCGCT
  CCTGGGGCAGCTTCACTGTGCTGGACAACTCGGCCTGGTACCAGAAGCAGCACCTGGTGGACTTCCTGGC
  GGCAGTGGGGGGTCACTTCCGCATGGGGACGCTGCTGAGCCGGCAGAGCGTGCAGCTGCGGCTCAAGAGC
  CCCGAGGGCATGAGCTTGGCCGAGTTCTTTTACCAGGTGCTCCAGGCCTATGACTTCTATTACCTCTTCC
  AGCGTTATGGATGCAGGGTCCAGCTGGGCGGATCTGATCAACTAGGCAACATCATGTCCGGATATGAGTT
  CATCAACAAGTTGACTGGAGAAGATGTATTTGGAATCACCGTTCCTCTAATTACAAGTACAACTGGAGCA
  AAGCTGGGAAAGTCTGCTGGCAACGCTGTTTGGCTAAACAGAGATAAGACATCTCCATTTGAATTGTATC
  AATTCTTTGTCAGGCAACCGGACGATTCAGTGGAAAGGTACCTGAAGCTGTTCACTTTCCTGCCCCTTCC
  AGAGATTGATCATATCATGCAGCTGCATGTCAAAGAGCCAGAAAGGCGGGGTCCTCAGAAACGACTGGCA
  GCAGAAGTAACAAAGCTTGTTCATGGACGAGAAGGATTGGATTCTGCTAAAAGGTGTACACAAGCCCTTT
  ATCACAGTAGCATAGATGCACTGGAGGTCATGTCTGATCAGGAGTTAAAAGAGTTGTTTAAAGAAGCTCC
  ATTTTCTGAATTTTTTCTCGATCCTGGAACAAGTGTCCTAGATACTTGCCGCAAAGCAAATGCCATTCCA
  GATGGTCCCCGAGGGTATCGAATGATAACAGAAGGCGGAGTCAGCATAAATCACCAACAAGTAACAAATC
  CTGAGAGTGTTTTAATTGTTGGACAACATATTCTCAAGAATGGACTTTCCTTACTTAAAATAGGAAAAAG
  AAATTTCTACATTATAAAATGGCTTCAGTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204828 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAPILRSFSWGRWSGTLNLSVLLPLGLRKAHSGAQGLLAAQKARGLFKDFFPETGTKIELPELFDRGTA
  SFPQTIYCGFDPTADSLHVGHLLALLGLFHLQRAGHNVIALVGGATARLGDPSGRTKEREALETERVRAN
  ARALRLGLEALAANHQQLFTDGRSWGSFTVLDNSAWYQKQHLVDFLAAVGGHFRMGTLLSRQSVQLRLKS
  PEGMSLAEFFYQVLQAYDFYYLFQRYGCRVQLGGSDQLGNIMSGYEFINKLTGEDVFGITVPLITSTTGA
  KLGKSAGNAVWLNRDKTSPFELYQFFVRQPDDSVERYLKLFTFLPLPEIDHIMQLHVKEPERRGPQKRLA
  AEVTKLVHGREGLDSAKRCTQALYHSSIDALEVMSDQELKELFKEAPFSEFFLDPGTSVLDTCRKANAIP
  DGPRGYRMITEGGVSINHQQVTNPESVLIVGQHILKNGLSLLKIGKRNFYIIKWLQL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001040436
• ORF Size: 1431 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001040436.3
• RefSeq Size: 2173 bp
• RefSeq ORF: 1434 bp
• Locus ID: 51067
• UniProt ID: Q9Y2Z4
• Cytogenetics: 12p11.21
• Protein Families: Druggable Genome
• Protein Pathways: Aminoacyl-tRNA biosynthesis
• MW: 53.2 kDa
• Gene Summary: This gene encodes a mitochondrial protein that catalyzes the attachment of tyrosine to tRNA(Tyr). Mutations in this gene are associated with myopathy with lactic acidosis and sideroblastic anemia type 2 (MLASA2). [provided by RefSeq, Jan 2011]