RRAS (NM_006270) Human Tagged ORF Clone

CAT#: RC204820

RRAS (Myc-DDK-tagged)-Human related RAS viral (r-ras) oncogene homolog (RRAS)

Product Images

Circular map for RC204820

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY RRAS (Cat# RC204820, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RRAS (Cat# TA809505)(1:2000). Positive lysates LY416758 (100ug) and LC416758 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY416758) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204820 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified RRAS protein (Cat# TP304820). The protein was produced from HEK293T cells transfected with RRAS cDNA clone (Cat# RC204820) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RRAS
• Synonyms: R-Ras
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204820 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAGCGGGGCGGCGTCCGGGACAGGGCGGGGGCGGCCCCGGGGCGGGGGACCTGGGCCCGGGGACC
  CCCCGCCCAGCGAGACACACAAGCTGGTGGTCGTGGGCGGCGGCGGCGTGGGCAAGAGCGCGCTGACCAT
  CCAGTTCATCCAGTCCTACTTCGTGTCTGACTACGACCCCACTATTGAGGACTCCTACACGAAGATCTGC
  AGTGTGGATGGCATCCCAGCCCGGCTGGACATCCTGGACACCGCGGGCCAGGAAGAGTTCGGGGCCATGA
  GAGAGCAGTACATGCGTGCTGGCCACGGCTTCCTGCTGGTGTTCGCCATTAACGACCGGCAGAGTTTCAA
  CGAGGTGGGCAAGCTCTTCACGCAGATTCTGCGGGTCAAGGACCGCGACGACTTCCCCGTTGTGTTGGTC
  GGGAACAAGGCAGATCTGGAGTCACAGCGCCAGGTCCCCCGATCAGAAGCCTCTGCCTTCGGCGCCTCCC
  ACCACGTGGCCTACTTTGAGGCCTCGGCCAAACTGCGTCTCAACGTGGACGAGGCTTTTGAGCAGCTGGT
  GCGGGCTGTCCGGAAATACCAGGAACAAGAGCTCCCACCGAGCCCTCCCAGTGCCCCCAGGAAGAAGGGC
  GGGGGCTGCCCCTGCGTCCTCCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204820 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSGAASGTGRGRPRGGGPGPGDPPPSETHKLVVVGGGGVGKSALTIQFIQSYFVSDYDPTIEDSYTKIC
  SVDGIPARLDILDTAGQEEFGAMREQYMRAGHGFLLVFAINDRQSFNEVGKLFTQILRVKDRDDFPVVLV
  GNKADLESQRQVPRSEASAFGASHHVAYFEASAKLRLNVDEAFEQLVRAVRKYQEQELPPSPPSAPRKKG
  GGCPCVLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006270
• ORF Size: 654 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006270.5
• RefSeq Size: 1013 bp
• RefSeq ORF: 657 bp
• Locus ID: 6237
• UniProt ID: P10301
• Cytogenetics: 19q13.33
• Domains: ras, RAN, RAS, RHO, RAB
• Protein Families: Druggable Genome
• Protein Pathways: MAPK signaling pathway, Regulation of actin cytoskeleton, Tight junction
• MW: 23.5 kDa
• Gene Summary: The protein encoded by this gene is a small GTPase involved in diverse processes including angiogenesis, vascular homeostasis and regeneration, cell adhesion, and neuronal axon guidance. Mutations in this gene are found in many invasive cancers. [provided by RefSeq, Jul 2015]