CDA08 (ITFG1) (NM_030790) Human Tagged ORF Clone

CAT#: RC204773

ITFG1 (Myc-DDK-tagged)-Human integrin alpha FG-GAP repeat containing 1 (ITFG1)

Product Images

Western blot validation of overexpression lysate (Cat# LY410712) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204773 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ITFG1 protein (Cat# TP304773). The protein was produced from HEK293T cells transfected with ITFG1 cDNA clone (Cat# RC204773) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CDA08
• Synonyms: 2310047C21Rik; CDA08; LNKN-1; TIP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204773 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCGGGCCGGCTCCCGAGCTCCTGGGCCCTCTTCTCGCCGCTCCTCGCAGGGCTTGCACTAC
  TGGGAGTCGGGCCGGTCCCAGCGCGGGCGCTGCACAACGTCACGGCCGAGCTCTTTGGGGCCGAGGCCTG
  GGGCACCCTTGCGGCTTTCGGGGACCTCAACTCCGACAAGCAGACGGATCTCTTCGTGCTGCGGGAAAGA
  AATGACTTAATCGTCTTTTTGGCAGACCAGAATGCACCCTATTTTAAACCCAAAGTAAAGGTATCTTTCA
  AGAATCACAGTGCATTGATAACAAGTGTAGTCCCTGGGGATTATGATGGAGATTCTCAAATGGATGTCCT
  TCTGACATATCTTCCCAAAAATTATGCCAAGAGTGAATTAGGAGCTGTTATCTTCTGGGGACAAAATCAA
  ACATTAGATCCTAACAATATGACCATACTCAATAGGACTTTTCAAGATGAGCCACTAATTATGGATTTCA
  ATGGTGATCTAATTCCTGATATTTTTGGTATCACAAATGAATCCAACCAGCCACAGATACTATTAGGAGG
  GAATTTATCATGGCATCCAGCATTGACCACTACAAGTAAAATGCGAATTCCACATTCTCATGCATTTATT
  GATCTGACTGAAGATTTTACAGCAGATTTATTCCTGACGACATTGAATGCCACCACTAGTACCTTCCAGT
  TTGAAATATGGGAAAATTTGGATGGAAACTTCTCTGTCAGTACTATATTGGAAAAACCTCAAAATATGAT
  GGTGGTTGGACAGTCAGCATTTGCAGACTTTGATGGAGATGGACACATGGATCATTTACTGCCAGGCTGT
  GAAGATAAAAATTGCCAAAAGAGTACCATCTACTTAGTGAGATCTGGGATGAAGCAGTGGGTTCCAGTCC
  TACAAGATTTCAGCAATAAGGGCACACTCTGGGGCTTTGTGCCATTTGTGGATGAACAGCAACCAACTGA
  AATACCAATTCCAATTACCCTTCATATTGGAGACTACAATATGGATGGCTATCCAGACGCTCTGGTCATA
  CTAAAGAACACATCTGGAAGCAACCAGCAGGCCTTTTTACTGGAGAACGTCCCTTGTAATAATGCAAGCT
  GTGAAGAGGCGCGTCGAATGTTTAAAGTCTACTGGGAGCTGACAGACCTAAATCAAATTAAGGATGCCAT
  GGTTGCCACCTTCTTTGACATTTACGAAGATGGAATCTTGGACATTGTAGTGCTAAGTAAAGGATATACA
  AAGAATGATTTTGCCATTCATACACTAAAAAATAACTTTGAAGCAGATGCTTATTTTGTTAAAGTTATTG
  TTCTTAGTGGTCTGTGTTCTAATGACTGTCCTCGTAAGATAACACCCTTTGGAGTGAATCAACCTGGACC
  TTATATCATGTATACAACTGTAGATGCAAATGGGTATCTGAAAAATGGATCAGCTGGCCAACTCAGCCAA
  TCCGCACATTTAGCTCTCCAACTACCATACAACGTGCTTGGTTTAGGTCGGAGCGCAAATTTTCTTGACC
  ATCTCTACGTTGGTATTCCCCGTCCATCTGGAGAAAAATCTATACGAAAACAAGAGTGGACTGCAATCAT
  TCCAAATTCCCAGCTAATTGTCATTCCATACCCTCACAATGTCCCTCGAAGTTGGAGTGCCAAACTGTAT
  CTTACACCAAGTAATATTGTTCTGCTTACTGCTATAGCTCTCATCGGTGTCTGTGTTTTCATCTTGGCAA
  TAATTGGCATTTTACATTGGCAGGAAAAGAAAGCAGATGATAGAGAAAAACGACAAGAAGCCCACCGGTT
  TCATTTTGATGCTATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204773 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAAGRLPSSWALFSPLLAGLALLGVGPVPARALHNVTAELFGAEAWGTLAAFGDLNSDKQTDLFVLRER
  NDLIVFLADQNAPYFKPKVKVSFKNHSALITSVVPGDYDGDSQMDVLLTYLPKNYAKSELGAVIFWGQNQ
  TLDPNNMTILNRTFQDEPLIMDFNGDLIPDIFGITNESNQPQILLGGNLSWHPALTTTSKMRIPHSHAFI
  DLTEDFTADLFLTTLNATTSTFQFEIWENLDGNFSVSTILEKPQNMMVVGQSAFADFDGDGHMDHLLPGC
  EDKNCQKSTIYLVRSGMKQWVPVLQDFSNKGTLWGFVPFVDEQQPTEIPIPITLHIGDYNMDGYPDALVI
  LKNTSGSNQQAFLLENVPCNNASCEEARRMFKVYWELTDLNQIKDAMVATFFDIYEDGILDIVVLSKGYT
  KNDFAIHTLKNNFEADAYFVKVIVLSGLCSNDCPRKITPFGVNQPGPYIMYTTVDANGYLKNGSAGQLSQ
  SAHLALQLPYNVLGLGRSANFLDHLYVGIPRPSGEKSIRKQEWTAIIPNSQLIVIPYPHNVPRSWSAKLY
  LTPSNIVLLTAIALIGVCVFILAIIGILHWQEKKADDREKRQEAHRFHFDAM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030790
• ORF Size: 1836 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030790.5
• RefSeq Size: 2261 bp
• RefSeq ORF: 1839 bp
• Locus ID: 81533
• UniProt ID: Q8TB96
• Cytogenetics: 16q12.1
• Domains: FG-GAP
• Protein Families: Secreted Protein, Transmembrane
• MW: 68.1 kDa
• Gene Summary: Modulator of T-cell function. Has a protective effect in graft versus host disease model (By similarity).[UniProtKB/Swiss-Prot Function]