SHKBP1 (NM_138392) Human Tagged ORF Clone
Lenti ORF clone of Human SH3KBP1 binding protein 1 (SHKBP1), Myc-DDK-tagged
|Product Name||SHKBP1 (NM_138392) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|E. coli Selection||Chloramphenicol (34 ug/mL)|
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene|
|ORF Size||2121 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at email@example.com or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||2379 bp|
|RefSeq ORF||2124 bp|
|Domains||BTB, WD40, K_tetra|
|Protein Families||Ion Channels: Other|
|Gene Summary||Inhibits CBL-SH3KBP1 complex mediated down-regulation of EGFR signaling by sequestration of SH3KBP1. Binds to SH3KBP1 and prevents its interaction with CBL and inhibits translocation of SH3KBP1 to EGFR containing vesicles upon EGF stimulation.[UniProtKB/Swiss-Prot Function]|
|cDNA Clone Resources|
|SC122216||SHKBP1 (untagged)-Human SH3KBP1 binding protein 1 (SHKBP1)||
|SC324510||SHKBP1 (untagged)-Human SH3KBP1 binding protein 1 (SHKBP1)||
|RC204760||SHKBP1 (Myc-DDK-tagged)-Human SH3KBP1 binding protein 1 (SHKBP1)||
|RG204760||SHKBP1 (GFP-tagged) - Human SH3KBP1 binding protein 1 (SHKBP1)||
|RC204760L1||Lenti ORF clone of Human SH3KBP1 binding protein 1 (SHKBP1), Myc-DDK-tagged||
|RC204760L2||Lenti ORF clone of Human SH3KBP1 binding protein 1 (SHKBP1), mGFP tagged||
|RC204760L4||Lenti ORF clone of Human SH3KBP1 binding protein 1 (SHKBP1), mGFP tagged||