SHKBP1 (NM_138392) Human Tagged ORF Clone

CAT#: RC204760

SHKBP1 (Myc-DDK-tagged)-Human SH3KBP1 binding protein 1 (SHKBP1)

Product images

Western blot validation of overexpression lysate (Cat# LY408656) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204760 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified SHKBP1 protein (Cat# TP304760). The protein was produced from HEK293T cells transfected with SHKBP1 cDNA clone (Cat# RC204760) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Product Name: SHKBP1 (NM_138392) Human Tagged ORF Clone
• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SHKBP1
• Synonyms: PP203; Sb1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204760 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCAGCGGCTACTGCAGCCGAGGGGGTCCCCAGTCGGGGGCCTCCCGGGGAAGTCATCCATCTGA
  ATGTGGGAGGCAAGAGATTCAGTACCTCTCGCCAGACTCTCACCTGGATCCCAGACTCCTTCTTCTCCAG
  TCTTCTGAGCGGACGCATCTCGACGCTGAAAGATGAGACCGGAGCAATCTTCATCGACAGGGACCCTACA
  GTCTTCGCCCCCATCCTCAACTTCCTGCGCACCAAAGAGTTGGATCCCAGGGGTGTCCACGGTTCCAGCC
  TCCTCCATGAAGCCCAGTTCTATGGGCTCACTCCTCTGGTTCGTCGCCTGCAGCTTCGAGAGGAGTTGGA
  TCGATCTTCTTGTGGAAACGTCCTCTTCAATGGTTACCTGCCGCCACCAGTGTTCCCAGTGAAGCGGCGG
  AACCGGCACAGCCTAGTGGGGCCTCAGCAGCTAGGAGGACGGCCAGCCCCTGTCCGACGGAGCAACACGA
  TGCCCCCCAACCTTGGCAATGCAGGGCTGCTGGGCCGAATGCTGGATGAGAAAACCCCTCCCTCACCCTC
  AGGACAACCTGAGGAGCCGGGGATGGTGCGCCTGGTGTGTGGACACCATAATTGGATCGCTGTGGCCTAT
  ACCCAGTTTCTAGTCTGCTACAGGTTGAAGGAAGCCTCTGGCTGGCAGCTGGTGTTTTCCAGCCCCCGCC
  TGGACTGGCCCATCGAACGACTGGCGCTCACAGCCCGGGTGCATGGTGGGGCTTTGGGTGAACATGACAA
  GATGGTGGCAGCAGCCACCGGCAGCGAGATCCTGCTATGGGCTCTGCAGGCGGAAGGCGGTGGCTCCGAG
  ATAGGGGTCTTTCATCTGGGGGTGCCTGTGGAGGCCTTGTTCTTCGTCGGGAACCAGCTCATTGCTACAA
  GCCACACAGGGCGCATCGGGGTGTGGAATGCCGTCACCAAGCACTGGCAGGTCCAGGAGGTGCAGCCCAT
  CACCAGTTATGACGCGGCAGGCTCCTTCCTCCTCCTGGGCTGCAACAACGGCTCCATTTACTACGTGGAT
  GTGCAGAAGTTCCCCTTGCGCATGAAAGACAACGACCTCCTTGTCAGCGAGCTCTATCGGGACCCAGCGG
  AGGATGGGGTCACCGCCCTCAGTGTCTACCTCACCCCCAAGACCAGTGACAGTGGGAACTGGATCGAGAT
  CGCCTATGGCACCAGCTCAGGGGGCGTGCGGGTCATCGTGCAGCACCCGGAGACTGTGGGCTCGGGGCCT
  CAGCTCTTCCAGACCTTCACTGTGCACCGCAGCCCTGTCACCAAGATCATGCTGTCGGAGAAGCACCTCA
  TCTCAGTCTGTGCCGACAACAACCACGTGCGGACATGGTCTGTGACTCGCTTCCGCGGCATGATTTCCAC
  CCAGCCCGGCTCCACCCCACTCGCTTCCTTTAAGATCCTGGCTCTGGAGTCGGCAGATGGGCATGGCGGC
  TGCAGTGCTGGCAATGACATTGGCCCCTACGGTGAGCGGGACGACCAGCTAGTGTTCATCCAGAAGGTGG
  TGCCCAGTGCCAGCCAGCTCTTCGTGCGTCTCTCATCTACTGGGCAGCGGGTGTGCTCCGTGCGCTCCGT
  GGACGGCTCACCCACGACGGCCTTCACAGTGCTGGAGTGCGAGGGCTCCCGGCGGCTCGGCTCTCGGCCC
  CGGCGCTACCTGCTCACTGGCCAGGCCAACGGCAGCTTGGCCATGTGGGACCTAACCACCGCCATGGACG
  GCCTCGGCCAGGCCCCTGCAGGTGGCCTGACGGAGCAAGAGCTGATGGAACAGCTGGAACACTGTGAGCT
  GGCCCCGCCGGCTCCTTCAGCTCCCTCATGGGGCTGTCTCCCCAGCCCCTCACCCCGCATCTCCCTCACC
  AGCCTCCACTCAGCCTCCAGCAACACCTCCTTGTCTGGCCACCGTGGGAGCCCAAGCCCCCCGCAGGCTG
  AGGCCCGGCGCCGTGGTGGGGGCAGCTTTGTGGAACGCTGCCAGGAACTGGTGCGGAGTGGGCCAGACCT
  CCGACGGCCACCCACACCAGCCCCGTGGCCCTCCAGCGGTCTCGGCACTCCCCTCACACCTCCCAAGATG
  AAGCTCAATGAAACTTCCTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204760 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAAATAAEGVPSRGPPGEVIHLNVGGKRFSTSRQTLTWIPDSFFSSLLSGRISTLKDETGAIFIDRDPT
  VFAPILNFLRTKELDPRGVHGSSLLHEAQFYGLTPLVRRLQLREELDRSSCGNVLFNGYLPPPVFPVKRR
  NRHSLVGPQQLGGRPAPVRRSNTMPPNLGNAGLLGRMLDEKTPPSPSGQPEEPGMVRLVCGHHNWIAVAY
  TQFLVCYRLKEASGWQLVFSSPRLDWPIERLALTARVHGGALGEHDKMVAAATGSEILLWALQAEGGGSE
  IGVFHLGVPVEALFFVGNQLIATSHTGRIGVWNAVTKHWQVQEVQPITSYDAAGSFLLLGCNNGSIYYVD
  VQKFPLRMKDNDLLVSELYRDPAEDGVTALSVYLTPKTSDSGNWIEIAYGTSSGGVRVIVQHPETVGSGP
  QLFQTFTVHRSPVTKIMLSEKHLISVCADNNHVRTWSVTRFRGMISTQPGSTPLASFKILALESADGHGG
  CSAGNDIGPYGERDDQLVFIQKVVPSASQLFVRLSSTGQRVCSVRSVDGSPTTAFTVLECEGSRRLGSRP
  RRYLLTGQANGSLAMWDLTTAMDGLGQAPAGGLTEQELMEQLEHCELAPPAPSAPSWGCLPSPSPRISLT
  SLHSASSNTSLSGHRGSPSPPQAEARRRGGGSFVERCQELVRSGPDLRRPPTPAPWPSSGLGTPLTPPKM
  KLNETSF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138392
• ORF Size: 2121 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138392.2
• RefSeq Size: 2379 bp
• RefSeq ORF: 2124 bp
• Locus ID: 92799
• Cytogenetics: 19q13.2
• Domains: BTB, WD40, K_tetra
• Protein Families: Ion Channels: Other
• MW: 76.3 kDa
• Gene Summary: Inhibits CBL-SH3KBP1 complex mediated down-regulation of EGFR signaling by sequestration of SH3KBP1. Binds to SH3KBP1 and prevents its interaction with CBL and inhibits translocation of SH3KBP1 to EGFR containing vesicles upon EGF stimulation.[UniProtKB/Swiss-Prot Function]