SHKBP1 (NM_138392) Human Tagged ORF Clone

USD 610.00


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Size
    • 10 ug


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Specifications

Product Data
Product Name SHKBP1 (NM_138392) Human Tagged ORF Clone
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SHKBP1
Synonyms PP203; Sb1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Cell Selection Neomycin
Sequence Data
>RC204760 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCAGCGGCTACTGCAGCCGAGGGGGTCCCCAGTCGGGGGCCTCCCGGGGAAGTCATCCATCTGA
ATGTGGGAGGCAAGAGATTCAGTACCTCTCGCCAGACTCTCACCTGGATCCCAGACTCCTTCTTCTCCAG
TCTTCTGAGCGGACGCATCTCGACGCTGAAAGATGAGACCGGAGCAATCTTCATCGACAGGGACCCTACA
GTCTTCGCCCCCATCCTCAACTTCCTGCGCACCAAAGAGTTGGATCCCAGGGGTGTCCACGGTTCCAGCC
TCCTCCATGAAGCCCAGTTCTATGGGCTCACTCCTCTGGTTCGTCGCCTGCAGCTTCGAGAGGAGTTGGA
TCGATCTTCTTGTGGAAACGTCCTCTTCAATGGTTACCTGCCGCCACCAGTGTTCCCAGTGAAGCGGCGG
AACCGGCACAGCCTAGTGGGGCCTCAGCAGCTAGGAGGACGGCCAGCCCCTGTCCGACGGAGCAACACGA
TGCCCCCCAACCTTGGCAATGCAGGGCTGCTGGGCCGAATGCTGGATGAGAAAACCCCTCCCTCACCCTC
AGGACAACCTGAGGAGCCGGGGATGGTGCGCCTGGTGTGTGGACACCATAATTGGATCGCTGTGGCCTAT
ACCCAGTTTCTAGTCTGCTACAGGTTGAAGGAAGCCTCTGGCTGGCAGCTGGTGTTTTCCAGCCCCCGCC
TGGACTGGCCCATCGAACGACTGGCGCTCACAGCCCGGGTGCATGGTGGGGCTTTGGGTGAACATGACAA
GATGGTGGCAGCAGCCACCGGCAGCGAGATCCTGCTATGGGCTCTGCAGGCGGAAGGCGGTGGCTCCGAG
ATAGGGGTCTTTCATCTGGGGGTGCCTGTGGAGGCCTTGTTCTTCGTCGGGAACCAGCTCATTGCTACAA
GCCACACAGGGCGCATCGGGGTGTGGAATGCCGTCACCAAGCACTGGCAGGTCCAGGAGGTGCAGCCCAT
CACCAGTTATGACGCGGCAGGCTCCTTCCTCCTCCTGGGCTGCAACAACGGCTCCATTTACTACGTGGAT
GTGCAGAAGTTCCCCTTGCGCATGAAAGACAACGACCTCCTTGTCAGCGAGCTCTATCGGGACCCAGCGG
AGGATGGGGTCACCGCCCTCAGTGTCTACCTCACCCCCAAGACCAGTGACAGTGGGAACTGGATCGAGAT
CGCCTATGGCACCAGCTCAGGGGGCGTGCGGGTCATCGTGCAGCACCCGGAGACTGTGGGCTCGGGGCCT
CAGCTCTTCCAGACCTTCACTGTGCACCGCAGCCCTGTCACCAAGATCATGCTGTCGGAGAAGCACCTCA
TCTCAGTCTGTGCCGACAACAACCACGTGCGGACATGGTCTGTGACTCGCTTCCGCGGCATGATTTCCAC
CCAGCCCGGCTCCACCCCACTCGCTTCCTTTAAGATCCTGGCTCTGGAGTCGGCAGATGGGCATGGCGGC
TGCAGTGCTGGCAATGACATTGGCCCCTACGGTGAGCGGGACGACCAGCTAGTGTTCATCCAGAAGGTGG
TGCCCAGTGCCAGCCAGCTCTTCGTGCGTCTCTCATCTACTGGGCAGCGGGTGTGCTCCGTGCGCTCCGT
GGACGGCTCACCCACGACGGCCTTCACAGTGCTGGAGTGCGAGGGCTCCCGGCGGCTCGGCTCTCGGCCC
CGGCGCTACCTGCTCACTGGCCAGGCCAACGGCAGCTTGGCCATGTGGGACCTAACCACCGCCATGGACG
GCCTCGGCCAGGCCCCTGCAGGTGGCCTGACGGAGCAAGAGCTGATGGAACAGCTGGAACACTGTGAGCT
GGCCCCGCCGGCTCCTTCAGCTCCCTCATGGGGCTGTCTCCCCAGCCCCTCACCCCGCATCTCCCTCACC
AGCCTCCACTCAGCCTCCAGCAACACCTCCTTGTCTGGCCACCGTGGGAGCCCAAGCCCCCCGCAGGCTG
AGGCCCGGCGCCGTGGTGGGGGCAGCTTTGTGGAACGCTGCCAGGAACTGGTGCGGAGTGGGCCAGACCT
CCGACGGCCACCCACACCAGCCCCGTGGCCCTCCAGCGGTCTCGGCACTCCCCTCACACCTCCCAAGATG
AAGCTCAATGAAACTTCCTTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC204760 protein sequence
Red=Cloning site Green=Tags(s)

MAAAATAAEGVPSRGPPGEVIHLNVGGKRFSTSRQTLTWIPDSFFSSLLSGRISTLKDETGAIFIDRDPT
VFAPILNFLRTKELDPRGVHGSSLLHEAQFYGLTPLVRRLQLREELDRSSCGNVLFNGYLPPPVFPVKRR
NRHSLVGPQQLGGRPAPVRRSNTMPPNLGNAGLLGRMLDEKTPPSPSGQPEEPGMVRLVCGHHNWIAVAY
TQFLVCYRLKEASGWQLVFSSPRLDWPIERLALTARVHGGALGEHDKMVAAATGSEILLWALQAEGGGSE
IGVFHLGVPVEALFFVGNQLIATSHTGRIGVWNAVTKHWQVQEVQPITSYDAAGSFLLLGCNNGSIYYVD
VQKFPLRMKDNDLLVSELYRDPAEDGVTALSVYLTPKTSDSGNWIEIAYGTSSGGVRVIVQHPETVGSGP
QLFQTFTVHRSPVTKIMLSEKHLISVCADNNHVRTWSVTRFRGMISTQPGSTPLASFKILALESADGHGG
CSAGNDIGPYGERDDQLVFIQKVVPSASQLFVRLSSTGQRVCSVRSVDGSPTTAFTVLECEGSRRLGSRP
RRYLLTGQANGSLAMWDLTTAMDGLGQAPAGGLTEQELMEQLEHCELAPPAPSAPSWGCLPSPSPRISLT
SLHSASSNTSLSGHRGSPSPPQAEARRRGGGSFVERCQELVRSGPDLRRPPTPAPWPSSGLGTPLTPPKM
KLNETSF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_138392
ORF Size 2121 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_138392.1, NM_138392.2, NM_138392.3, NP_612401.1
RefSeq Size 2379 bp
RefSeq ORF 2124 bp
Locus ID 92799
Cytogenetics 19q13.2
Domains BTB, WD40, K_tetra
Protein Families Ion Channels: Other
MW 76.3 kDa

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