UBE1C (UBA3) (NM_003968) Human Tagged ORF Clone

CAT#: RC204675

UBA3 (Myc-DDK-tagged)-Human ubiquitin-like modifier activating enzyme 3 (UBA3), transcript variant 1

Product Images

Circular map for RC204675

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY UBA3 (Cat# RC204675, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UBA3(Cat# TA811903). Positive lysates LY418315 (100ug) and LC418315 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY418315) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204675 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified UBA3 protein (Cat# TP304675). The protein was produced from HEK293T cells transfected with UBA3 cDNA clone (Cat# RC204675) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UBE1C
• Synonyms: hUBA3; NAE2; UBE1C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204675 representing NM_003968
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGATGGCGAGGAGCCGGAGAGGAAAAGAAGGAGAATAGAGGAGCTGCTGGCTGAGAAAATGGCTG
  TTGATGGTGGGTGTGGGGACACTGGAGACTGGGAAGGTCGCTGGAACCATGTAAAGAAGTTCCTCGAGCG
  ATCTGGACCCTTCACACACCCTGATTTCGAACCGAGCACTGAATCTCTCCAGTTCTTGTTAGATACATGT
  AAAGTTCTAGTCATTGGAGCTGGCGGCTTAGGATGTGAGCTCCTGAAAAATCTGGCCTTGTCTGGTTTTA
  GACAGATTCATGTTATAGATATGGACACTATAGATGTTTCCAATCTAAATAGGCAGTTTTTATTTAGGCC
  TAAAGATATTGGAAGACCTAAGGCTGAAGTTGCTGCAGAATTTCTAAATGACAGAGTTCCTAATTGCAAT
  GTAGTTCCACATTTCAACAAGATTCAAGATTTTAACGACACTTTCTATCGACAATTTCATATTATTGTAT
  GTGGACTGGACTCTATCATCGCCAGAAGATGGATAAATGGCATGCTGATATCTCTTCTAAATTATGAAGA
  TGGTGTCTTAGATCCAAGCTCCATTGTCCCTTTGATAGATGGGGGGACAGAAGGTTTTAAAGGAAATGCC
  CGGGTGATTCTGCCTGGAATGACTGCTTGTATCGAATGCACGCTGGAACTTTATCCACCACAGGTTAATT
  TTCCCATGTGCACCATTGCATCTATGCCCAGGCTACCAGAACACTGTATTGAGTATGTAAGGATGTTGCA
  GTGGCCTAAGGAGCAGCCTTTTGGAGAAGGGGTTCCATTAGATGGAGATGATCCTGAACATATACAATGG
  ATTTTCCAAAAATCCCTAGAGAGAGCATCACAATATAATATTAGGGGTGTTACGTATAGGCTCACTCAAG
  GGGTAGTAAAAAGAATCATTCCTGCAGTAGCTTCCACAAATGCAGTCATTGCAGCTGTGTGTGCCACTGA
  GGTTTTTAAAATAGCCACAAGTGCATACATTCCCTTGAATAATTACTTGGTGTTTAATGATGTAGATGGG
  CTGTATACATACACATTTGAAGCAGAAAGAAAGGAAAACTGCCCAGCTTGTAGCCAGCTTCCTCAAAATA
  TTCAGTTTTCTCCATCAGCTAAACTACAGGAGGTTTTGGATTATCTAACCAATAGTGCTTCTCTGCAAAT
  GAAATCTCCAGCCATCACAGCCACCCTAGAGGGAAAAAATAGAACACTTTACTTACAGTCGGTAACCTCT
  ATTGAAGAACGAACAAGGCCAAATCTCTCCAAAACATTGAAAGAATTGGGGCTTGTTGATGGACAAGAAC
  TGGCGGTTGCTGATGTCACCACCCCACAGACTGTACTATTCAAACTTCATTTTACTTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204675 representing NM_003968
  Red=Cloning site Green=Tags(s)
  
  MADGEEPERKRRRIEELLAEKMAVDGGCGDTGDWEGRWNHVKKFLERSGPFTHPDFEPSTESLQFLLDTC
  KVLVIGAGGLGCELLKNLALSGFRQIHVIDMDTIDVSNLNRQFLFRPKDIGRPKAEVAAEFLNDRVPNCN
  VVPHFNKIQDFNDTFYRQFHIIVCGLDSIIARRWINGMLISLLNYEDGVLDPSSIVPLIDGGTEGFKGNA
  RVILPGMTACIECTLELYPPQVNFPMCTIASMPRLPEHCIEYVRMLQWPKEQPFGEGVPLDGDDPEHIQW
  IFQKSLERASQYNIRGVTYRLTQGVVKRIIPAVASTNAVIAAVCATEVFKIATSAYIPLNNYLVFNDVDG
  LYTYTFEAERKENCPACSQLPQNIQFSPSAKLQEVLDYLTNSASLQMKSPAITATLEGKNRTLYLQSVTS
  IEERTRPNLSKTLKELGLVDGQELAVADVTTPQTVLFKLHFTS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003968
• ORF Size: 1389 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003968.4
• RefSeq Size: 2136 bp
• RefSeq ORF: 1392 bp
• Locus ID: 9039
• UniProt ID: Q8TBC4
• Cytogenetics: 3p14.1
• Domains: UBACT, ThiF
• Protein Pathways: Ubiquitin mediated proteolysis
• MW: 51.7 kDa
• Gene Summary: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]