Tyrosyl tRNA synthetase (YARS) (NM_003680) Human Tagged ORF Clone

CAT#: RC204590

YARS (Myc-DDK-tagged)-Human tyrosyl-tRNA synthetase (YARS)

Product Images

Circular map for RC204590

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  Western blot validation of overexpression lysate (Cat# LY418498) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204590 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified YARS protein (Cat# TP304590). The protein was produced from HEK293T cells transfected with YARS cDNA clone (Cat# RC204590) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Tyrosyl tRNA synthetase
• Synonyms: CMTDIC; TYRRS; YARS; YRS; YTS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204590 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGACGCTCCCAGCCCTGAAGAGAAACTGCACCTTATCACCCGGAACCTGCAGGAGGTTCTGGGGG
  AAGAGAAGCTGAAGGAGATACTGAAGGAGCGGGAACTTAAAATTTACTGGGGAACGGCAACCACGGGCAA
  ACCACATGTGGCTTACTTTGTGCCCATGTCAAAGATTGCAGACTTCTTAAAGGCAGGGTGTGAGGTAACA
  ATTCTGTTTGCGGACCTCCACGCATACCTGGATAACATGAAAGCCCCATGGGAACTTCTAGAACTCCGAG
  TCAGTTACTATGAGAATGTGATCAAAGCAATGCTGGAGAGCATTGGTGTGCCCTTGGAGAAGCTCAAGTT
  CATCAAAGGCACTGATTACCAGCTCAGCAAAGAGTACACACTAGATGTGTACAGACTCTCCTCCGTGGTC
  ACACAGCACGATTCCAAGAAGGCTGGAGCTGAGGTGGTAAAGCAGGTGGAGCACCCTTTGCTGAGTGGCC
  TCTTATACCCCGGACTGCAGGCTTTGGATGAAGAGTATTTAAAAGTAGATGCCCAATTTGGAGGCATTGA
  TCAGAGAAAGATTTTCACCTTTGCAGAGAAGTACCTCCCTGCACTTGGCTATTCAAAACGGGTCCATCTG
  ATGAATCCTATGGTTCCAGGATTAACAGGCAGCAAAATGAGCTCTTCAGAAGAGGAGTCCAAGATTGATC
  TCCTTGATCGGAAGGAGGATGTGAAGAAAAAACTGAAGAAGGCCTTCTGTGAGCCAGGAAATGTGGAGAA
  CAATGGGGTTCTGTCCTTCATCAAGCATGTCCTTTTTCCCCTTAAGTCCGAGTTTGTGATCCTACGAGAT
  GAGAAATGGGGTGGAAACAAAACCTACACAGCTTACGTGGACCTGGAAAAGGACTTTGCTGCTGAGGTTG
  TACATCCTGGAGACCTGAAGAATTCTGTTGAAGTCGCACTGAACAAGTTGCTGGATCCAATCCGGGAAAA
  GTTTAATACCCCTGCCCTGAAAAAACTGGCCAGCGCTGCCTACCCAGATCCCTCAAAGCAGAAGCCAATG
  GCCAAAGGCCCTGCCAAGAATTCAGAACCAGAGGAGGTCATCCCATCCCGGCTGGATATCCGTGTGGGGA
  AAATCATCACTGTGGAGAAGCACCCAGATGCAGACAGCCTGTATGTAGAGAAGATTGACGTGGGGGAAGC
  TGAACCACGGACTGTGGTGAGCGGCCTGGTACAGTTCGTGCCCAAGGAGGAACTGCAGGACAGGCTGGTA
  GTGGTGCTGTGCAACCTGAAACCCCAGAAGATGAGAGGAGTCGAGTCCCAAGGCATGCTTCTGTGTGCTT
  CTATAGAAGGGATAAACCGCCAGGTTGAACCTCTGGACCCTCCGGCAGGCTCTGCTCCTGGTGAGCACGT
  GTTTGTGAAGGGCTATGAAAAGGGCCAACCAGATGAGGAGCTCAAGCCCAAGAAGAAAGTCTTCGAGAAG
  TTGCAGGCTGACTTCAAAATTTCTGAGGAGTGCATCGCACAGTGGAAGCAAACCAACTTCATGACCAAGC
  TGGGCTCCATTTCCTGTAAATCGCTGAAAGGGGGGAACATTAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204590 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGDAPSPEEKLHLITRNLQEVLGEEKLKEILKERELKIYWGTATTGKPHVAYFVPMSKIADFLKAGCEVT
  ILFADLHAYLDNMKAPWELLELRVSYYENVIKAMLESIGVPLEKLKFIKGTDYQLSKEYTLDVYRLSSVV
  TQHDSKKAGAEVVKQVEHPLLSGLLYPGLQALDEEYLKVDAQFGGIDQRKIFTFAEKYLPALGYSKRVHL
  MNPMVPGLTGSKMSSSEEESKIDLLDRKEDVKKKLKKAFCEPGNVENNGVLSFIKHVLFPLKSEFVILRD
  EKWGGNKTYTAYVDLEKDFAAEVVHPGDLKNSVEVALNKLLDPIREKFNTPALKKLASAAYPDPSKQKPM
  AKGPAKNSEPEEVIPSRLDIRVGKIITVEKHPDADSLYVEKIDVGEAEPRTVVSGLVQFVPKEELQDRLV
  VVLCNLKPQKMRGVESQGMLLCASIEGINRQVEPLDPPAGSAPGEHVFVKGYEKGQPDEELKPKKKVFEK
  LQADFKISEECIAQWKQTNFMTKLGSISCKSLKGGNIS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003680
• ORF Size: 1584 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003680.3
• RefSeq Size: 3117 bp
• RefSeq ORF: 1587 bp
• Locus ID: 8565
• UniProt ID: P54577
• Cytogenetics: 1p35.1
• Domains: tRNA-synt_1b, tRNA_bind
• Protein Families: Druggable Genome
• Protein Pathways: Aminoacyl-tRNA biosynthesis
• MW: 59.1 kDa
• Gene Summary: Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine. [provided by RefSeq, Jul 2008]