RNF125 (NM_017831) Human Tagged ORF Clone

CAT#: RC204578

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RNF125 (Myc-DDK-tagged)-Human ring finger protein 125 (RNF125)



  "NM_017831" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 118.00

USD 429.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol RNF125
Synonyms TNORS; TRAC-1; TRAC1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC204578 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
GCC
C

ATGGGCTCCGTGCTGAGCACCGACAGCGGCAAATCGGCGCCCGCCTCTGCCACCGCGCGGGCCCTGGAGC
GCAGGAGGGACCCGGAGTTGCCCGTCACGTCCTTCGACTGCGCCGTGTGCCTTGAGGTGTTACACCAGCC
TGTCCGGACCCGCTGTGGCCACGTATTCTGCCGTTCCTGTATTGCTACCAGTCTAAAGAACAACAAGTGG
ACCTGTCCTTATTGCCGGGCATATCTTCCTTCAGAAGGAGTTCCAGCAACTGATGTAGCCAAAAGAATGA
AATCAGAGTATAAGAACTGCGCTGAGTGTGACATAGTTCTTTACCTCAGTGAAATGAGGGCACATATTCG
GACTTGTCAGAAGTACATAGATAAGTATGGACCACTACAAGAACTTGAGGAGACAGCAGCAAGGTGTGTA
TGTCCCTTTTGTCAGAGGGAACTGTATGAAGACAGCTTGCTGGATCATTGTATTACTCATCACAGATCGG
AACGGAGGCCTGTGTTCTGTCCACTTTGCCGTTTAATACCCGATGAGAATCCAAGCAGCTTCAGTGGCAG
TTTAATAAGACATCTGCAAGTTAGTCACACTTTGTTTTATGATGATTTCATAGATTTTAATATAATTGAG
GAAGCTCTTATCCGAAGAGTCTTAGACCGGTCACTTCTTGAATATGTGAATCACTCGAACACAGCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC204578 protein sequence
Red=Cloning site Green=Tags(s)

MGSVLSTDSGKSAPASATARALERRRDPELPVTSFDCAVCLEVLHQPVRTRCGHVFCRSCIATSLKNNKW
TCPYCRAYLPSEGVPATDVAKRMKSEYKNCAECDIVLYLSEMRAHIRTCQKYIDKYGPLQELEETAARCV
CPFCQRELYEDSLLDHCITHHRSERRPVFCPLCRLIPDENPSSFSGSLIRHLQVSHTLFYDDFIDFNIIE
EALIRRVLDRSLLEYVNHSNTA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites AscI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_017831
ORF Size 696 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_017831.4
RefSeq Size 5888 bp
RefSeq ORF 699 bp
Locus ID 54941
UniProt ID Q96EQ8
Cytogenetics 18q12.1
Domains RING
Protein Families Druggable Genome
Protein Pathways RIG-I-like receptor signaling pathway
MW 26.5 kDa
Gene Summary This gene encodes a novel E3 ubiquitin ligase that contains a RING finger domain in the N-terminus and three zinc-binding and one ubiquitin-interacting motif in the C-terminus. As a result of myristoylation, this protein associates with membranes and is primarily localized to intracellular membrane systems. The encoded protein may function as a positive regulator in the T-cell receptor signaling pathway. [provided by RefSeq, Mar 2012]

Other Versions

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