RNF125 (NM_017831) Human Tagged ORF Clone

CAT#: RC204578

RNF125 (Myc-DDK-tagged)-Human ring finger protein 125 (RNF125)

Product Images

Western blot validation of overexpression lysate (Cat# LY413505) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204578 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RNF125
• Synonyms: TNORS; TRAC-1; TRAC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204578 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGGGCTCCGTGCTGAGCACCGACAGCGGCAAATCGGCGCCCGCCTCTGCCACCGCGCGGGCCCTGGAGC
  GCAGGAGGGACCCGGAGTTGCCCGTCACGTCCTTCGACTGCGCCGTGTGCCTTGAGGTGTTACACCAGCC
  TGTCCGGACCCGCTGTGGCCACGTATTCTGCCGTTCCTGTATTGCTACCAGTCTAAAGAACAACAAGTGG
  ACCTGTCCTTATTGCCGGGCATATCTTCCTTCAGAAGGAGTTCCAGCAACTGATGTAGCCAAAAGAATGA
  AATCAGAGTATAAGAACTGCGCTGAGTGTGACATAGTTCTTTACCTCAGTGAAATGAGGGCACATATTCG
  GACTTGTCAGAAGTACATAGATAAGTATGGACCACTACAAGAACTTGAGGAGACAGCAGCAAGGTGTGTA
  TGTCCCTTTTGTCAGAGGGAACTGTATGAAGACAGCTTGCTGGATCATTGTATTACTCATCACAGATCGG
  AACGGAGGCCTGTGTTCTGTCCACTTTGCCGTTTAATACCCGATGAGAATCCAAGCAGCTTCAGTGGCAG
  TTTAATAAGACATCTGCAAGTTAGTCACACTTTGTTTTATGATGATTTCATAGATTTTAATATAATTGAG
  GAAGCTCTTATCCGAAGAGTCTTAGACCGGTCACTTCTTGAATATGTGAATCACTCGAACACAGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204578 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGSVLSTDSGKSAPASATARALERRRDPELPVTSFDCAVCLEVLHQPVRTRCGHVFCRSCIATSLKNNKW
  TCPYCRAYLPSEGVPATDVAKRMKSEYKNCAECDIVLYLSEMRAHIRTCQKYIDKYGPLQELEETAARCV
  CPFCQRELYEDSLLDHCITHHRSERRPVFCPLCRLIPDENPSSFSGSLIRHLQVSHTLFYDDFIDFNIIE
  EALIRRVLDRSLLEYVNHSNTA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017831
• ORF Size: 696 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017831.4
• RefSeq Size: 5888 bp
• RefSeq ORF: 699 bp
• Locus ID: 54941
• UniProt ID: Q96EQ8
• Cytogenetics: 18q12.1
• Domains: RING
• Protein Families: Druggable Genome
• Protein Pathways: RIG-I-like receptor signaling pathway
• MW: 26.5 kDa
• Gene Summary: This gene encodes a novel E3 ubiquitin ligase that contains a RING finger domain in the N-terminus and three zinc-binding and one ubiquitin-interacting motif in the C-terminus. As a result of myristoylation, this protein associates with membranes and is primarily localized to intracellular membrane systems. The encoded protein may function as a positive regulator in the T-cell receptor signaling pathway. [provided by RefSeq, Mar 2012]