PPWD1 (NM_015342) Human Tagged ORF Clone

CAT#: RC204570

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PPWD1 (Myc-DDK-tagged)-Human peptidylprolyl isomerase domain and WD repeat containing 1 (PPWD1)



Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 118.00

USD 880.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PPWD1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC204570 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGGAAAGTGGTAGCGATTTTCAGCAGAGACGTAGAAGGCGCCGGGACCCGGAGGAACCGGAAA
AAACAGAACTCAGCGAAAGAGAGCTGGCAGTAGCAGTGGCGGTGTCCCAGGAGAACGATGAGGAGAACGA
AGAGCGCTGGGTTGGACCTTTACCTGTGGAGGCAACACTGGCCAAGAAGAGGAAAGTCTTAGAGTTTGAA
AGAGTCTATCTTGATAATCTCCCCAGTGCATCCATGTATGAGCGCAGTTACATGCATAGAGATGTTATCA
CCCATGTGGTATGCACCAAAACAGATTTTATTATTACTGCCAGTCATGATGGACATGTCAAGTTCTGGAA
AAAAATAGAAGAGGGAATTGAATTTGTTAAACATTTTCGTAGTCACCTGGGAGTTATTGAGAGTATTGCA
GTTAGCTCTGAGGGAGCATTGTTCTGTTCTGTGGGTGATGATAAAGCAATGAAGGTGTTTGATGTAGTGA
ACTTTGACATGATCAACATGCTGAAACTTGGCTATTTTCCTGGACAGTGTGAGTGGATCTATTGCCCAGG
GGATGCAATTTCTTCAGTTGCTGCTTCCGAAAAGAGTACAGGAAAAATTTTCATTTATGATGGCCGAGGA
GATAACCAGCCACTTCATATTTTTGACAAACTCCATACATCACCTCTTACTCAGATACGGCTAAACCCAG
TTTACAAAGCAGTAGTGTCTTCTGACAAATCTGGGATGATTGAATACTGGACTGGGCCTCCTCATGAATA
TAAATTCCCCAAAAATGTGAACTGGGAATATAAAACTGACACTGATTTATATGAATTTGCCAAGTGTAAG
GCTTATCCAACCAGCGTATGTTTTTCACCAGATGGGAAGAAAATAGCTACTATTGGTTCTGATAGAAAAG
TTAGAATTTTCAGATTTGTAACTGGAAAACTCATGAGAGTCTTTGATGAATCACTAAGCATGTTTACTGA
ACTGCAACAGATGAGGCAACAGTTACCAGACATGGAATTTGGCCGACGAATGGCTGTAGAACGTGAGTTG
GAGAAGGTTGATGCTGTAAGATTAATTAATATAGTTTTTGATGAAACTGGACACTTCGTGCTGTATGGAA
CAATGCTGGGCATTAAAGTTATAAATGTAGAGACAAACCGGTGTGTGCGGATTTTAGGCAAACAAGAAAA
TATTAGAGTGATGCAATTGGCTTTGTTCCAGGGGATAGCCAAAAAGCATCGTGCTGCAACTACTATAGAA
ATGAAAGCTTCTGAAAATCCTGTTCTTCAGAATATTCAAGCTGACCCAACAATAGTCTGTACATCATTCA
AAAAGAATAGATTTTATATGTTTACCAAACGAGAACCAGAAGATACGAAAAGTGCAGATTCTGATCGAGA
TGTTTTTAATGAGAAACCTTCTAAAGAAGAAGTCATGGCAGCTACTCAAGCTGAAGGACCTAAACGAGTT
TCGGACAGTGCCATTATCCACACCAGCATGGGAGACATTCACACCAAACTTTTTCCTGTTGAGTGCCCTA
AGACAGTGGAAAACTTCTGTGTTCACAGCAGAAATGGTTATTATAATGGGCATACATTTCACCGTATAAT
TAAGGGCTTTATGATTCAGACTGGAGATCCAACAGGTACTGGTATGGGAGGAGAAAGCATATGGGGAGGA
GAATTTGAAGATGAATTTCATTCAACATTACGACATGACAGGCCGTACACACTCAGCATGGCTAACGCGG
GATCAAATACTAATGGATCCCAGTTTTTCATAACGGTAGTACCAACGCCTTGGCTTGATAATAAGCATAC
AGTATTTGGACGAGTGACTAAAGGAATGGAAGTTGTACAGAGGATCTCCAACGTCAAAGTCAATCCCAAA
ACAGATAAGCCCTATGAGGATGTCAGCATCATAAATATTACTGTCAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC204570 protein sequence
Red=Cloning site Green=Tags(s)

MAAESGSDFQQRRRRRRDPEEPEKTELSERELAVAVAVSQENDEENEERWVGPLPVEATLAKKRKVLEFE
RVYLDNLPSASMYERSYMHRDVITHVVCTKTDFIITASHDGHVKFWKKIEEGIEFVKHFRSHLGVIESIA
VSSEGALFCSVGDDKAMKVFDVVNFDMINMLKLGYFPGQCEWIYCPGDAISSVAASEKSTGKIFIYDGRG
DNQPLHIFDKLHTSPLTQIRLNPVYKAVVSSDKSGMIEYWTGPPHEYKFPKNVNWEYKTDTDLYEFAKCK
AYPTSVCFSPDGKKIATIGSDRKVRIFRFVTGKLMRVFDESLSMFTELQQMRQQLPDMEFGRRMAVEREL
EKVDAVRLINIVFDETGHFVLYGTMLGIKVINVETNRCVRILGKQENIRVMQLALFQGIAKKHRAATTIE
MKASENPVLQNIQADPTIVCTSFKKNRFYMFTKREPEDTKSADSDRDVFNEKPSKEEVMAATQAEGPKRV
SDSAIIHTSMGDIHTKLFPVECPKTVENFCVHSRNGYYNGHTFHRIIKGFMIQTGDPTGTGMGGESIWGG
EFEDEFHSTLRHDRPYTLSMANAGSNTNGSQFFITVVPTPWLDNKHTVFGRVTKGMEVVQRISNVKVNPK
TDKPYEDVSIINITVK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_015342
ORF Size 1938 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_015342.1, NP_056157.1
RefSeq Size 2186 bp
RefSeq ORF 1941 bp
Locus ID 23398
UniProt ID Q96BP3
Cytogenetics 5q12.3
MW 73.6 kDa
Gene Summary PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding (PubMed:20676357). May be involved in pre-mRNA splicing (PubMed:11991638).[UniProtKB/Swiss-Prot Function]
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