FGF21 (NM_019113) Human Tagged ORF Clone
FGF21 (Myc-DDK-tagged)-Human fibroblast growth factor 21 (FGF21)
|Product Name||FGF21 (NM_019113) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC204538 representing NM_019113
Red=Cloning site Blue=ORF Green=Tags(s)
>RC204538 representing NM_019113
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||627 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at email@example.com or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||940 bp|
|RefSeq ORF||630 bp|
|Protein Families||Adult stem cells, Embryonic stem cells, ES Cell Differentiation/IPS, Secreted Protein|
|Protein Pathways||MAPK signaling pathway, Melanoma, Pathways in cancer, Regulation of actin cytoskeleton|
|Gene Summary||Theis gene encodes a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes. This protein is a secreted endocrine factor that functions as a major metabolic regulator. The encoded protein stimulates the uptake of glucose in adipose tissue. [provided by RefSeq, Mar 2016]|
|cDNA Clone Resources|
|SC304675||FGF21 (untagged)-Human fibroblast growth factor 21 (FGF21)||
|RG204538||FGF21 (GFP-tagged) - Human fibroblast growth factor 21 (FGF21)||
|RC204538L1||Lenti ORF clone of Human fibroblast growth factor 21 (FGF21), Myc-DDK-tagged||
|RC204538L2||Lenti ORF clone of Human fibroblast growth factor 21 (FGF21), mGFP tagged||
|RC204538L3||Lenti ORF clone of Human fibroblast growth factor 21 (FGF21), Myc-DDK-tagged||
|RC204538L4||Lenti ORF clone of Human fibroblast growth factor 21 (FGF21), mGFP tagged||