Pannexin 1 (PANX1) (NM_015368) Human Tagged ORF Clone

CAT#: RC204474

PANX1 (Myc-DDK-tagged)-Human pannexin 1 (PANX1)

Product Images

Circular map for RC204474

• 

  Western blot validation of overexpression lysate (Cat# LY402428) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204474 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pannexin 1
• Synonyms: MRS1; OOMD7; PX1; UNQ2529
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204474 representing NM_015368
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCATCGCTCACCTGGCCACGGAGTACGTGTTCTCGGATTTCTTGCTGAAGGAGCCCACGGAGCCCA
  AGTTCAAGGGGCTGCGACTGGAGCTGGCTGTGGACAAGATGGTCACGTGCATTGCGGTGGGGCTGCCCCT
  GCTGCTCATCTCGCTGGCCTTCGCGCAGGAGATCTCGATTGGTACACAGATAAGCTGTTTCTCTCCAAGT
  TCTTTCTCCTGGCGTCAGGCTGCCTTTGTGGATTCATATTGCTGGGCGGCTGTTCAGCAGAAGAACTCAC
  TGCAGAGCGAGTCTGGAAACCTCCCACTGTGGCTGCATAAGTTTTTCCCCTACATCCTGCTGCTCTTTGC
  GATCCTCCTGTACCTGCCCCCGCTGTTCTGGCGTTTCGCAGCTGCTCCTCATATTTGCTCAGACTTGAAG
  TTTATCATGGAAGAACTTGACAAAGTTTACAACCGTGCAATTAAGGCTGCAAAGAGTGCGCGTGACCTTG
  ACATGAGAGATGGAGCCTGCTCAGTTCCAGGTGTTACCGAGAACTTAGGGCAAAGTTTGTGGGAGGTATC
  TGAAAGCCACTTCAAGTACCCAATTGTGGAGCAGTACTTGAAGACAAAGAAAAATTCTAATAATTTAATC
  ATCAAGTACATTAGCTGCCGCCTGCTGACACTCATCATTATACTGTTAGCGTGTATCTACCTGGGCTATT
  ACTTCAGCCTCTCCTCACTCTCAGACGAGTTTGTGTGCAGCATCAAATCAGGGATCCTGAGAAACGACAG
  CACCGTGCCCGATCAGTTTCAGTGCAAACTCATTGCCGTGGGCATCTTCCAGTTGCTCAGTGTCATTAAC
  CTTGTGGTTTATGTCCTGCTGGCTCCCGTGGTTGTCTACACGCTGTTTGTTCCATTCCGACAGAAGACAG
  ATGTTCTCAAAGTGTACGAAATCCTCCCCACTTTTGATGTTCTGCATTTCAAATCTGAAGGGTACAACGA
  TTTGAGCCTCTACAATCTCTTCTTGGAGGAAAATATAAGTGAGGTCAAGTCATACAAGTGTCTTAAGGTA
  CTGGAGAATATTAAGAGCAGTGGTCAGGGGATCGACCCAATGCTACTCCTGACAAACCTTGGCATGATCA
  AGATGGATGTTGTTGATGGCAAAACTCCCATGTCTGCAGAGATGAGAGAGGAGCAGGGGAACCAGACGGC
  AGAGCTCCAAGGTATGAACATAGACAGTGAAACTAAAGCAAATAATGGAGAGAAGAATGCCCGACAGAGA
  CTTCTGGATTCTTCTTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204474 representing NM_015368
  Red=Cloning site Green=Tags(s)
  
  MAIAHLATEYVFSDFLLKEPTEPKFKGLRLELAVDKMVTCIAVGLPLLLISLAFAQEISIGTQISCFSPS
  SFSWRQAAFVDSYCWAAVQQKNSLQSESGNLPLWLHKFFPYILLLFAILLYLPPLFWRFAAAPHICSDLK
  FIMEELDKVYNRAIKAAKSARDLDMRDGACSVPGVTENLGQSLWEVSESHFKYPIVEQYLKTKKNSNNLI
  IKYISCRLLTLIIILLACIYLGYYFSLSSLSDEFVCSIKSGILRNDSTVPDQFQCKLIAVGIFQLLSVIN
  LVVYVLLAPVVVYTLFVPFRQKTDVLKVYEILPTFDVLHFKSEGYNDLSLYNLFLEENISEVKSYKCLKV
  LENIKSSGQGIDPMLLLTNLGMIKMDVVDGKTPMSAEMREEQGNQTAELQGMNIDSETKANNGEKNARQR
  LLDSSC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015368
• ORF Size: 1278 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015368.3, NP_056183.2
• RefSeq Size: 2782 bp
• RefSeq ORF: 1281 bp
• Locus ID: 24145
• UniProt ID: Q96RD7
• Cytogenetics: 11q21
• Protein Families: Transmembrane
• MW: 47.9 kDa
• Gene Summary: The protein encoded by this gene belongs to the innexin family. Innexin family members are the structural components of gap junctions. This protein and pannexin 2 are abundantly expressed in central nerve system (CNS) and are coexpressed in various neuronal populations. Studies in Xenopus oocytes suggest that this protein alone and in combination with pannexin 2 may form cell type-specific gap junctions with distinct properties. [provided by RefSeq, Jul 2008]