Carboxypeptidase B (CPB1) (NM_001871) Human Tagged ORF Clone

CAT#: RC204463

CPB1 (Myc-DDK-tagged)-Human carboxypeptidase B1 (tissue) (CPB1)

Product Images

Circular map for RC204463

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  Western blot validation of overexpression lysate (Cat# LY419699) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204463 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CPB1 protein (Cat# TP304463). The protein was produced from HEK293T cells transfected with CPB1 cDNA clone (Cat# RC204463) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Carboxypeptidase B
• Synonyms: CPB; PASP; PCPB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204463 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGGCACTCTTGGTTCTGGTGACTGTGGCCCTGGCATCTGCTCATCATGGTGGTGAGCACTTTGAAG
  GCGAGAAGGTGTTCCGTGTTAACGTTGAAGATGAAAATCACATTAACATAATCCGCGAGTTGGCCAGCAC
  GACCCAGATTGACTTCTGGAAGCCAGATTCTGTCACACAAATCAAACCTCACAGTACAGTTGACTTCCGT
  GTTAAAGCAGAAGATACTGTCACTGTGGAGAATGTTCTAAAGCAGAATGAACTACAATACAAGGTACTGA
  TAAGCAACCTGAGAAATGTGGTGGAGGCTCAGTTTGATAGCCGGGTTCGTGCAACAGGACACAGTTATGA
  GAAGTACAACAAGTGGGAAACGATAGAGGCTTGGACTCAACAAGTCGCCACTGAGAATCCAGCCCTCATC
  TCTCGCAGTGTTATCGGAACCACATTTGAGGGACGCGCTATTTACCTCCTGAAGGTTGGCAAAGCTGGAC
  AAAATAAGCCTGCCATTTTCATGGACTGTGGTTTCCATGCCAGAGAGTGGATTTCTCCTGCATTCTGCCA
  GTGGTTTGTAAGAGAGGCTGTTCGTACCTATGGACGTGAGATCCAAGTGACAGAGCTTCTCGACAAGTTA
  GACTTTTATGTCCTGCCTGTGCTCAATATTGATGGCTACATCTACACCTGGACCAAGAGCCGATTTTGGA
  GAAAGACTCGCTCCACCCATACTGGATCTAGCTGCATTGGCACAGACCCCAACAGAAATTTTGATGCTGG
  TTGGTGTGAAATTGGAGCCTCTCGAAACCCCTGTGATGAAACTTACTGTGGACCTGCCGCAGAGTCTGAA
  AAGGAGACCAAGGCCCTGGCTGATTTCATCCGCAACAAACTCTCTTCCATCAAGGCATATCTGACAATCC
  ACTCGTACTCCCAAATGATGATCTACCCTTACTCATATGCTTACAAACTCGGTGAGAACAATGCTGAGTT
  GAATGCCCTGGCTAAAGCTACTGTGAAAGAACTTGCCTCACTGCACGGCACCAAGTACACATATGGCCCG
  GGAGCTACAACAATCTATCCTGCTGCTGGGGGCTCTGACGACTGGGCTTATGACCAAGGAATCAGATATT
  CCTTCACCTTTGAACTTCGAGATACAGGCAGATATGGCTTTCTCCTTCCAGAATCCCAGATCCGGGCTAC
  CTGCGAGGAGACCTTCCTGGCAATCAAGTATGTTGCCAGCTACGTCCTGGAACACCTGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204463 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLALLVLVTVALASAHHGGEHFEGEKVFRVNVEDENHINIIRELASTTQIDFWKPDSVTQIKPHSTVDFR
  VKAEDTVTVENVLKQNELQYKVLISNLRNVVEAQFDSRVRATGHSYEKYNKWETIEAWTQQVATENPALI
  SRSVIGTTFEGRAIYLLKVGKAGQNKPAIFMDCGFHAREWISPAFCQWFVREAVRTYGREIQVTELLDKL
  DFYVLPVLNIDGYIYTWTKSRFWRKTRSTHTGSSCIGTDPNRNFDAGWCEIGASRNPCDETYCGPAAESE
  KETKALADFIRNKLSSIKAYLTIHSYSQMMIYPYSYAYKLGENNAELNALAKATVKELASLHGTKYTYGP
  GATTIYPAAGGSDDWAYDQGIRYSFTFELRDTGRYGFLLPESQIRATCEETFLAIKYVASYVLEHLY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001871
• ORF Size: 1251 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001871.3
• RefSeq Size: 1473 bp
• RefSeq ORF: 1254 bp
• Locus ID: 1360
• UniProt ID: P15086
• Cytogenetics: 3q24
• Domains: Zn_carbOpept, Propep_M14
• Protein Families: Druggable Genome, Protease, Secreted Protein
• MW: 47.4 kDa
• Gene Summary: Three different procarboxypeptidases A and two different procarboxypeptidases B have been isolated. The B1 and B2 forms differ from each other mainly in isoelectric point. Carboxypeptidase B1 is a highly tissue-specific protein and is a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants. It is not elevated in pancreatic carcinoma. [provided by RefSeq, Jul 2008]