SLC2A6 (NM_017585) Human Tagged ORF Clone

CAT#: RC204391

SLC2A6 (Myc-DDK-tagged)-Human solute carrier family 2 (facilitated glucose transporter), member 6 (SLC2A6), transcript variant 1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SLC2A6 (Cat# RC204391, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC2A6(Cat# TA500639). Positive lysates LY402605 (100ug) and LC402605 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY402605) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204391 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified SLC2A6 protein (Cat# TP304391). The protein was produced from HEK293T cells transfected with SLC2A6 cDNA clone (Cat# RC204391) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC2A6
• Synonyms: GLUT6; GLUT9; HSA011372
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204391 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGAGCCGCTGCTGGGAGCCGAGGGCCCGGACTACGACACCTTCCCCGAGAAGCCGCCCCCGTCGC
  CAGGGGACAGGGCGCGGGTCGGGACCCTGCAGAACAAAAGGGTGTTCCTGGCCACCTTCGCCGCAGTGCT
  CGGCAATTTCAGCTTTGGGTATGCCCTGGTCTACACATCCCCTGTCATCCCAGCCCTGGAGCGCTCCTTG
  GATCCTGACCTGCATCTGACCAAATCCCAGGCATCCTGGTTTGGGTCCGTGTTCACCCTGGGAGCAGCGG
  CCGGAGGCCTGAGTGCCATGATCCTCAACGACCTCCTGGGCCGGAAGCTGAGCATCATGTTCTCAGCTGT
  GCCGTCGGCGGCCGGCTATGCGCTCATGGCGGGTGCGCACGGCCTCTGGATGCTGCTGCTCGGAAGGACG
  CTGACGGGCTTCGCCGGGGGGCTCACAGCTGCCTGCATCCCGGTGTACGTGTCTGAGATTGCTCCCCCAG
  GCGTTCGTGGGGCTCTGGGGGCCACACCCCAGCTCATGGCAGTGTTCGGATCCCTGTCCCTCTACGCCCT
  TGGCCTCCTGCTGCCGTGGCGCTGGCTGGCTGTGGCCGGGGAGGCGCCTGTGCTCATCATGATCCTGCTG
  CTCAGCTTCATGCCCAACTCGCCGCGCTTCCTGCTCTCTCGGGGCAGGGACGAAGAGGCCCTGCGGGCGC
  TGGCCTGGCTGCGTGGGACGGACGTCGATGTCCACTGGGAGTTCGAGCAGATCCAGGACAACGTCCGGAG
  ACAGAGCAGCCGAGTATCGTGGGCTGAGGCACGGGCCCCCCACGTGTGCCGGCCCATCACCGTGGCCTTG
  CTGATGCGCCTCCTGCAGCAGCTGACGGGCATCACGCCCATCCTGGTCTACCTGCAGTCCATCTTCGACA
  GCACCGCTGTCCTGCTGCCCCCCAAGGACGACGCAGCCATCGTTGGGGCCGTGCGGCTCCTGTCCGTGCT
  GATCGCCGCCCTCACCATGGACCTCGCAGGCCGCAAGGTGCTGCTCTTCGTCTCAGCGGCCATCATGTTT
  GCTGCCAACCTGACTCTGGGGCTGTACATCCACTTTGGCCCCAGGCCTCTGAGCCCCAACAGCACTGCGG
  GCCTGGAAAGCGAGTCCTGGGGGGACTTGGCGCAGCCCCTGGCAGCACCCGCTGGCTACCTCACCCTGGT
  GCCCCTGCTGGCCACCATGCTCTTCATCATGGGCTACGCCGTGGGCTGGGGTCCCATCACCTGGCTGCTC
  ATGTCTGAGGTCCTGCCCCTGCGTGCCCGTGGCGTGGCCTCAGGGCTCTGCGTGCTGGCCAGCTGGCTCA
  CCGCCTTCGTCCTCACCAAGTCCTTCCTGCCAGTGGTGAGCACCTTCGGCCTCCAGGTGCCTTTCTTCTT
  CTTCGCGGCCATCTGCTTGGTGAGCCTGGTGTTCACAGGCTGCTGTGTGCCCGAGACCAAGGGACGGTCC
  CTGGAGCAGATCGAGTCCTTCTTCCGCACGGGGAGAAGGTCCTTCTTGCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204391 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQEPLLGAEGPDYDTFPEKPPPSPGDRARVGTLQNKRVFLATFAAVLGNFSFGYALVYTSPVIPALERSL
  DPDLHLTKSQASWFGSVFTLGAAAGGLSAMILNDLLGRKLSIMFSAVPSAAGYALMAGAHGLWMLLLGRT
  LTGFAGGLTAACIPVYVSEIAPPGVRGALGATPQLMAVFGSLSLYALGLLLPWRWLAVAGEAPVLIMILL
  LSFMPNSPRFLLSRGRDEEALRALAWLRGTDVDVHWEFEQIQDNVRRQSSRVSWAEARAPHVCRPITVAL
  LMRLLQQLTGITPILVYLQSIFDSTAVLLPPKDDAAIVGAVRLLSVLIAALTMDLAGRKVLLFVSAAIMF
  AANLTLGLYIHFGPRPLSPNSTAGLESESWGDLAQPLAAPAGYLTLVPLLATMLFIMGYAVGWGPITWLL
  MSEVLPLRARGVASGLCVLASWLTAFVLTKSFLPVVSTFGLQVPFFFFAAICLVSLVFTGCCVPETKGRS
  LEQIESFFRTGRRSFLR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017585
• ORF Size: 1521 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017585.4
• RefSeq Size: 2563 bp
• RefSeq ORF: 1524 bp
• Locus ID: 11182
• UniProt ID: Q9UGQ3
• Cytogenetics: 9q34.2
• Protein Families: Transmembrane
• MW: 54.5 kDa
• Gene Summary: Hexose transport into mammalian cells is catalyzed by a family of membrane proteins, including SLC2A6, that contain 12 transmembrane domains and a number of critical conserved residues.[supplied by OMIM, Jul 2002]