ZDHHC11 (NM_024786) Human Tagged ORF Clone

CAT#: RC204383

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ZDHHC11 (Myc-DDK-tagged)-Human zinc finger, DHHC-type containing 11 (ZDHHC11)



  "NM_024786" in other vectors (4)

Reconstitution Protocol

USD 457.00


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In Stock

Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol ZDHHC11
Synonyms ZNF399
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC204383 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACACCCGCTCCGGGAGCCAGTGTTCCGTCACCCCAGAAGCCATACTCAATAATGAAAAGCTGGTCT
TGCCGCCCCGCATCTCCAGAGTGAACGGCTGGTCGTTACCCCTGCACTACTTCCAGGTGGTGACCTGGGC
TGTCTTCGTGGGCCTTTCCTCGGCCACCTTCGGGATCTTCATTCCCTTCCTGCCTCACGCGTGGAAATAC
ATTGCCTACGTGGTGACCGGGGGGATCTTCTCGTTCCACCTCGTCGTCCACCTGATCGCGTCCTGCATCG
ACCCGGCCGACTCCAATGTCAGACTCATGAAGAACTATTCTCAGCCCATGCCCCTCTTCGACAGATCAAA
ACATGCACACGTGATCCAGAATCAGTTCTGCCACCTGTGCAAGGTCACCGTGAACAAGAAAACCAAACAC
TGCATTTCCTGCAATAAGTGTGTGTCCGGCTTCGACCACCACTGCAAATGGATCAACAACTGCGTGGGAA
GCCGGAATTATTGGTTCTTCTTCAGCACTGTGGCCTCGGCCACAGCTGGCATGCTCTGCCTGATCGCCAT
CCTGCTGTATGTCCTCGTCCAGTACCTCGTGAACCCCGGGGTGCTCCGCACGGACCCCAGGTATGAAGAT
GTCAAGAATATGAACACGTGGCTGCTGTTCCTCCCCCTGTTCCCGGTGCAGGTGCAGACCCTGATAGTCG
TGATCATCGGGATGCTCGTGCTCCTGCTGGACTTTCTTGGCTTGGTGCACCTGGGCCAGCTGCTCATCTT
CCACATCTACCTGAAGGCCAAGAAGATGACCACCTTTGAGTATCTCATTAATAACCGCAAAGAAGAGAGT
TCAAAACATCAAGCAGTGAGGAAAGATCCATACGTGCAAATGGACAAAGGAGTTCTCCAGCAAGGAGCTG
GCGCCCTGGGCTCATCTGCACAGGGAGTCAAAGCCAAGAGCTCCCTGCTGATTCACAAGCACTTATGTCA
CTTCTGCACTTCAGTAAACCAGGATGGGGATTCGACGGCACGGGAAGGGGATGAAGACCCGTGTCCATCT
GCACTTGGAGCCAAGGCCAGGAACTCCCGGCTGATTTGCAGGCGCCTGTGTCAGTTCTCCACTCGTGTAC
ACCCAGACGGGGGCTCGATGGCACAGGAAGCAGATGATGCCCCGAGTATATCTACACTTGGGCTGCAACA
AGAAACAACAGAGCCCATGAAAACTGACAGTGCTGAAAGTGAAGAC


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
>RC204383 protein sequence
Red=Cloning site Green=Tags(s)

MDTRSGSQCSVTPEAILNNEKLVLPPRISRVNGWSLPLHYFQVVTWAVFVGLSSATFGIFIPFLPHAWKY
IAYVVTGGIFSFHLVVHLIASCIDPADSNVRLMKNYSQPMPLFDRSKHAHVIQNQFCHLCKVTVNKKTKH
CISCNKCVSGFDHHCKWINNCVGSRNYWFFFSTVASATAGMLCLIAILLYVLVQYLVNPGVLRTDPRYED
VKNMNTWLLFLPLFPVQVQTLIVVIIGMLVLLLDFLGLVHLGQLLIFHIYLKAKKMTTFEYLINNRKEES
SKHQAVRKDPYVQMDKGVLQQGAGALGSSAQGVKAKSSLLIHKHLCHFCTSVNQDGDSTAREGDEDPCPS
ALGAKARNSRLICRRLCQFSTRVHPDGGSMAQEADDAPSISTLGLQQETTEPMKTDSAESED

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_024786
ORF Size 1236 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_024786.2
RefSeq Size 2618 bp
RefSeq ORF 1239 bp
Locus ID 79844
UniProt ID Q9H8X9
Cytogenetics 5p15.33
Protein Families Transmembrane
MW 46 kDa
Gene Summary Endoplasmic reticulum-localized palmitoyltransferase that could catalyze the addition of palmitate onto various protein substrates and be involved in a variety of cellular processes (By similarity). Has a palmitoyltransferase activity toward NCDN and regulates NCDN association with endosome membranes through this palmitoylation (By similarity). May play a role in cell proliferation (PubMed:28331227).[UniProtKB/Swiss-Prot Function]

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