CALML5 (NM_017422) Human Tagged ORF Clone

CAT#: RC204372

CALML5 (Myc-DDK-tagged)-Human calmodulin-like 5 (CALML5)

Product Images

Circular map for RC204372

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  Western blot validation of overexpression lysate (Cat# LY413780) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204372 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CALML5 protein (Cat# TP304372). The protein was produced from HEK293T cells transfected with CALML5 cDNA clone (Cat# RC204372) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CALML5
• Synonyms: CLSP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204372 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGTGAGCTGACTCCTGAGGAGGAGGCCCAGTACAAAAAGGCTTTCTCCGCGGTTGACACGGATG
  GAAACGGCACCATCAATGCCCAGGAGCTGGGCGCGGCGCTGAAGGCCACGGGCAAGAACCTCTCGGAGGC
  CCAGCTAAGGAAACTCATCTCCGAGGTTGACGGCGACGGCGACGGCGAAATCAGCTTCCAGGAGTTCCTG
  ACGGCGGCAAGGAAGGCCAGGGCCGGCCTGGAGGACCTGCAGGTCGCCTTCCGCGCCTTCGACCAGGATG
  GCGACGGCCACATCACCGTGGACGAGCTCAGGCGGGCCATGGCGGGGCTGGGGCAGCCGCTGCCGCAGGA
  GGAGCTGGACGCCATGATCCGCGAGGCCGACGTGGACCAGGACGGGCGGGTGAACTACGAGGAGTTCGCG
  AGGATGCTCGCCCAGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204372 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGELTPEEEAQYKKAFSAVDTDGNGTINAQELGAALKATGKNLSEAQLRKLISEVDGDGDGEISFQEFL
  TAARKARAGLEDLQVAFRAFDQDGDGHITVDELRRAMAGLGQPLPQEELDAMIREADVDQDGRVNYEEFA
  RMLAQE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017422
• ORF Size: 438 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017422.5
• RefSeq Size: 893 bp
• RefSeq ORF: 441 bp
• Locus ID: 51806
• UniProt ID: Q9NZT1
• Cytogenetics: 10p15.1
• Protein Pathways: Alzheimer's disease, Calcium signaling pathway, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term potentiation, Melanogenesis, Neurotrophin signaling pathway, Olfactory transduction, Oocyte meiosis, Phosphatidylinositol signaling system, Vascular smooth muscle contraction
• MW: 15.9 kDa
• Gene Summary: This gene encodes a novel calcium binding protein expressed in the epidermis and related to the calmodulin family of calcium binding proteins. Functional studies with recombinant protein demonstrate it does bind calcium and undergoes a conformational change when it does so. Abundant expression is detected only in reconstructed epidermis and is restricted to differentiating keratinocytes. In addition, it can associate with transglutaminase 3, shown to be a key enzyme in the terminal differentiation of keratinocytes. [provided by RefSeq, Jul 2008]