HuC (ELAVL3) (NM_001420) Human Tagged ORF Clone

CAT#: RC204323

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ELAVL3 (Myc-DDK-tagged)-Human ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C) (ELAVL3), transcript variant 1



  "NM_001420" in other vectors (4)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol ELAVL3
Synonyms HUC; HUCL; PLE21
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC204323 representing NM_001420
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTCACTCAGATACTGGGGGCCATGGAGTCTCAGGTGGGGGGGGGCCCGGCCGGCCCGGCCCTGCCCA
ACGGGCCACTCCTTGGTACAAATGGAGCCACTGACGACAGCAAGACCAACCTCATCGTCAACTACCTGCC
CCAGAACATGACCCAGGATGAGTTCAAGAGTCTCTTCGGCAGCATTGGCGACATCGAGTCCTGCAAGTTG
GTTCGGGACAAGATCACAGGGCAGAGCCTTGGCTACGGGTTTGTGAACTATTCTGACCCCAATGATGCAG
ACAAAGCCATCAACACCCTCAACGGCCTCAAATTACAGACGAAGACCATCAAGGTGTCCTATGCCAGACC
CAGTTCAGCATCCATCCGGGATGCTAACCTGTACGTCAGCGGGCTCCCCAAGACCATGAGCCAGAAAGAG
ATGGAGCAGCTCTTCTCCCAGTACGGCCGCATCATCACGTCCCGCATCCTGGTGGACCAGGTCACAGGTG
TCTCTCGGGGTGTGGGATTCATCCGCTTTGACAAGAGGATTGAGGCCGAAGAGGCTATCAAAGGACTGAA
TGGGCAGAAGCCGCTGGGCGCAGCTGAGCCCATCACAGTCAAGTTCGCGAACAACCCAAGTCAGAAGACG
GGGCAGGCGCTGCTCACCCACCTCTACCAGTCATCCGCCCGGCGCTACGCAGGCCCCCTACACCATCAGA
CCCAGCGTTTCCGGCTGGACAATTTGCTCAACATGGCCTACGGCGTCAAGAGTCCCCTGTCGCTCATCGC
CAGGTTCTCGCCGATCGCCATCGATGGTATGAGCGGCCTGGCGGGCGTGGGCCTGTCGGGGGGCGCGGCG
GGCGCCGGCTGGTGCATCTTCGTGTACAACCTGTCACCGGAGGCAGACGAGAGCGTGCTGTGGCAGCTGT
TCGGGCCTTTTGGGGCAGTCACCAACGTCAAGGTCATCCGTGATTTCACCACCAACAAGTGCAAGGGTTT
CGGCTTCGTGACCATGACCAACTATGACGAGGCGGCCATGGCCATCGCCAGCCTGAACGGCTATCGCCTG
GGCGAGCGCGTGCTGCAGGTCTCCTTCAAGACCAGCAAACAGCACAAGGCG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC204323 representing NM_001420
Red=Cloning site Green=Tags(s)

MVTQILGAMESQVGGGPAGPALPNGPLLGTNGATDDSKTNLIVNYLPQNMTQDEFKSLFGSIGDIESCKL
VRDKITGQSLGYGFVNYSDPNDADKAINTLNGLKLQTKTIKVSYARPSSASIRDANLYVSGLPKTMSQKE
MEQLFSQYGRIITSRILVDQVTGVSRGVGFIRFDKRIEAEEAIKGLNGQKPLGAAEPITVKFANNPSQKT
GQALLTHLYQSSARRYAGPLHHQTQRFRLDNLLNMAYGVKSPLSLIARFSPIAIDGMSGLAGVGLSGGAA
GAGWCIFVYNLSPEADESVLWQLFGPFGAVTNVKVIRDFTTNKCKGFGFVTMTNYDEAAMAIASLNGYRL
GERVLQVSFKTSKQHKA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001420
ORF Size 1101 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001420.4
RefSeq Size 4682 bp
RefSeq ORF 1104 bp
Locus ID 1995
UniProt ID Q14576
Cytogenetics 19p13.2
MW 39.4 kDa
Gene Summary A member of the ELAVL protein family, ELAV-like 3 is a neural-specific RNA-binding protein which contains three RNP-type RNA recognition motifs. The observation that ELAVL3 is one of several Hu antigens (neuronal-specific RNA-binding proteins) recognized by the anti-Hu serum antibody present in sera from patients with paraneoplastic encephalomyelitis and sensory neuronopathy (PEM/PSN) suggests it has a role in neurogenesis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

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