OSBP1 (OSBP) (NM_002556) Human Tagged ORF Clone

CAT#: RC204250

OSBP (Myc-DDK-tagged)-Human oxysterol binding protein (OSBP)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY OSBP (Cat# RC204250, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OSBP(Cat# TA503140). Positive lysates LY419260 (100ug) and LC419260 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY419260) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204250 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified OSBP protein (Cat# TP304250). The protein was produced from HEK293T cells transfected with OSBP cDNA clone (Cat# RC204250) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OSBP1
• Synonyms: OSBP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204250 representing NM_002556
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGACGGAGCTGAGAGGAGTGGTGGGGCCAGGCCCGGCAGCCATTGCAGCACTTGGCGGCGGCG
  GCGCCGGTCCCCCAGTGGTGGGAGGAGGCGGCGGCCGCGGAGATGCGGGGCCAGGCTCCGGGGCCGCGTC
  AGGGACGGTGGTCGCGGCGGCGGCGGGAGGCCCGGGCCCGGGGGCCGGGGGAGTGGCGGCGGCTGGCCCG
  GCCCCTGCGCCGCCGACTGGGGGCTCGGGCGGCTCGGGCGCTGGGGGTTCGGGCTCGGCTCGAGAGGGCT
  GGCTCTTCAAATGGACCAATTATATCAAAGGCTACCAGCGGCGATGGTTCGTGCTGAGCAACGGGCTCCT
  GAGCTACTACAGATCAAAGGCAGAAATGAGACATACCTGCCGTGGTACCATCAACCTCGCCACAGCCAAC
  ATCACCGTGGAGGACTCCTGCAACTTCATCATTTCCAATGGGGGTGCTCAGACCTACCATCTGAAAGCTA
  GTTCAGAAGTTGAGCGGCAGCGCTGGGTGACGGCCCTGGAACTGGCCAAGGCCAAAGCTGTGAAGATGCT
  GGCAGAGTCAGATGAATCAGGAGATGAAGAGTCTGTCTCACAAACTGACAAGACTGAGCTGCAGAATACC
  CTTCGGACCCTCTCTAGCAAAGTAGAGGACTTGAGCACGTGCAATGACTTGATAGCTAAGCATGGCACAG
  CTCTGCAGCGTTCTCTCAGTGAGCTGGAGTCCCTGAAGTTGCCTGCTGAGAGCAATGAAAAGATCAAACA
  GGTCAACGAACGAGCCACACTCTTTAGGATAACATCCAATGCCATGATCAACGCCTGCAGAGATTTCCTC
  ATGTTAGCCCAGACCCATAGTAAAAAATGGCAAAAGTCACTACAGTATGAAAGAGACCAGCGTATCCGAC
  TGGAAGAAACCCTCGAGCAGCTGGCGAAGCAGCATAATCACCTGGAGAGGGCCTTCCGAGGAGCCACGGT
  GCTGCCGGCAAACACTCCTGGCAATGTGGGTTCTGGTAAAGATCAGTGCTGCTCTGGCAAAGGGGACATG
  AGCGATGAAGATGATGAGAATGAATTTTTTGATGCACCTGAGATCATCACCATGCCTGAAAATTTGGGCC
  ACAAACGTACTGGCAGCAATATCAGTGGAGCCAGCAGTGACATCAGCCTTGATGAACAGTACAAGCATCA
  GCTGGAGGAGACCAAAAAGGAAAAGAGAACCAGAATACCATACAAGCCAAACTATAGCCTCAATTTATGG
  AGCATCATGAAGAACTGCATTGGAAAAGAACTCTCTAAGATCCCCATGCCGGTAAACTTTAATGAGCCCT
  TGTCCATGCTTCAGCGCCTTACTGAAGATCTGGAATACCATGAGCTGTTAGACCGAGCTGCAAAATGTGA
  GAATTCTCTAGAACAGCTCTGTTATGTTGCAGCTTTCACCGTGTCCTCCTACTCCACTACTGTCTTCCGC
  ACCAGTAAGCCATTCAACCCACTGCTTGGGGAGACCTTTGAGCTGGACCGATTAGAGGAGAATGGGTACC
  GATCCCTCTGTGAACAGGTGAGTCATCATCCCCCTGCTGCTGCGCACCATGCTGAGTCCAAAAATGGCTG
  GACATTGCGTCAGGAAATCAAAATCACCAGCAAGTTTCGAGGCAAATACCTCTCCATTATGCCCCTCGGT
  ACCATTCATTGTATTTTCCATGCAACTGGGCACCACTACACTTGGAAGAAAGTTACCACAACTGTACACA
  ACATTATTGTGGGCAAGTTGTGGATAGATCAGTCTGGCGAAATTGATATTGTGAATCACAAGACAGGAGA
  CAAGTGTAATCTTAAATTTGTTCCTTATAGCTACTTCTCTCGGGATGTAGCAAGAAAGGTGACGGGGGAA
  GTGACAGATCCATCAGGAAAAGTCCACTTTGCTCTTCTGGGGACGTGGGATGAGAAAATGGAATGTTTCA
  AAGTACAGCCAGTCATTGGGGAAAATGGGGGTGATGCTCGACAGAGAGGCCATGAAGCAGAGGAAAGCAG
  GGTCATGCTGTGGAAAAGGAATCCTTTACCGAAGAATGCAGAAAACATGTACTACTTCTCAGAGCTTGCT
  CTGACTCTCAATGCTTGGGAAAGTGGCACTGCCCCCACAGACAGCCGGTTACGACCTGACCAGAGACTGA
  TGGAAAATGGACGCTGGGATGAAGCAAATGCGGAGAAGCAGCGCCTGGAGGAAAAACAAAGACTTTCCAG
  AAAGAAGAGAGAAGCGGAAGCTATGAAAGCCACAGAGGATGGCACACCATATGATCCCTATAAGGCACTG
  TGGTTTGAGCGGAAGAAGGACCCTGTTACCAAGGAGTTAACCCATATTTATAGGGGAGAATACTGGGAGT
  GTAAAGAAAAACAGGACTGGAGCTCATGCCCGGACATTTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204250 representing NM_002556
  Red=Cloning site Green=Tags(s)
  
  MAATELRGVVGPGPAAIAALGGGGAGPPVVGGGGGRGDAGPGSGAASGTVVAAAAGGPGPGAGGVAAAGP
  APAPPTGGSGGSGAGGSGSAREGWLFKWTNYIKGYQRRWFVLSNGLLSYYRSKAEMRHTCRGTINLATAN
  ITVEDSCNFIISNGGAQTYHLKASSEVERQRWVTALELAKAKAVKMLAESDESGDEESVSQTDKTELQNT
  LRTLSSKVEDLSTCNDLIAKHGTALQRSLSELESLKLPAESNEKIKQVNERATLFRITSNAMINACRDFL
  MLAQTHSKKWQKSLQYERDQRIRLEETLEQLAKQHNHLERAFRGATVLPANTPGNVGSGKDQCCSGKGDM
  SDEDDENEFFDAPEIITMPENLGHKRTGSNISGASSDISLDEQYKHQLEETKKEKRTRIPYKPNYSLNLW
  SIMKNCIGKELSKIPMPVNFNEPLSMLQRLTEDLEYHELLDRAAKCENSLEQLCYVAAFTVSSYSTTVFR
  TSKPFNPLLGETFELDRLEENGYRSLCEQVSHHPPAAAHHAESKNGWTLRQEIKITSKFRGKYLSIMPLG
  TIHCIFHATGHHYTWKKVTTTVHNIIVGKLWIDQSGEIDIVNHKTGDKCNLKFVPYSYFSRDVARKVTGE
  VTDPSGKVHFALLGTWDEKMECFKVQPVIGENGGDARQRGHEAEESRVMLWKRNPLPKNAENMYYFSELA
  LTLNAWESGTAPTDSRLRPDQRLMENGRWDEANAEKQRLEEKQRLSRKKREAEAMKATEDGTPYDPYKAL
  WFERKKDPVTKELTHIYRGEYWECKEKQDWSSCPDIF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002556
• ORF Size: 2421 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002556.3
• RefSeq Size: 5083 bp
• RefSeq ORF: 2424 bp
• Locus ID: 5007
• UniProt ID: P22059
• Cytogenetics: 11q12.1
• Domains: Oxysterol_BP, PH
• Protein Families: Druggable Genome
• MW: 89.2 kDa
• Gene Summary: Oxysterol binding protein is an intracellular protein that is believed to transport sterols from lysosomes to the nucleus where the sterol down-regulates the genes for the LDL receptor, HMG-CoA reductase, and HMG synthetase [provided by RefSeq, Jul 2008]