ZBTB2 (NM_020861) Human Tagged ORF Clone

CAT#: RC204198

ZBTB2 (Myc-DDK-tagged)-Human zinc finger and BTB domain containing 2 (ZBTB2)

Product Images

Circular map for RC204198

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  Western blot validation of overexpression lysate (Cat# LY412230) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204198 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ZBTB2 protein (Cat# TP304198). The protein was produced from HEK293T cells transfected with ZBTB2 cDNA clone (Cat# RC204198) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZBTB2
• Synonyms: ZNF437
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204198 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTTGGCCAACCATGGACTTATTCTACTGCAACAGTTAAACGCTCAGCGAGAGTTTGGTTTCCTGT
  GTGACTGCACGGTTGCAATCGGCGATGTATACTTCAAGGCACACAAATCAGTTCTTGCTTCATTCTCCAA
  TTACTTTAAGATGTTGTTTGTCCATCAGACCAGTGAGTGTGTCCGCTTGAAACCAACTGACATACAGCCC
  GACATCTTCAGCTATCTCTTACACTTGATGTACACTGGGAAGATGGCGCCTCAGCTCATCGACCCGGTTC
  GATTAGAACAGGGCATCAAGTTTCTGCACGCCTACCCGCTCATTCAGGAAGCCAGCCTCGCCAGCCAGGG
  AGCCTTTTCTCACCCTGACCAAGTTTTCCCACTGGCTTCTTCATTGTATGGCATTCAGATTGCAGATCAT
  CAGTTGAGACAAGCCACCAAGATTGCTTCAGCACCTGAAAAACTCGGGCGAGATCCACGGCCACAGACCT
  CCAGGATAAGCCAGGAGCAGGTCCCTGAGGCCTCACAGCTCTCCCAGCTGACTTCAAATCTGGCCCAGGT
  GAATCGGACAAATATGACTCCCTCAGACCCCCTGCAGACCTCGCTGTCTCCAGAACTTGTTTCCACTCCT
  GTTCCTCCCCCTCCTCCCGGGGAGGAGACCAATCTGGAAGCATCTTCCTCCGATGAGCAGCCTGCGTCCC
  TCACAATAGCCCACGTCAAGCCAAGCATCATGAAGAGGAATGGGAGCTTTCCAAAGTACTATGCCTGCCA
  CCTGTGTGGACGGCGCTTCACTCTCCGGAGCAGCTTACGTGAACACCTCCAGATCCACACAGGAGTACCT
  TTCACATCTAGCCAACAGGGAGAAAGTCGCGTCCCCCTGACTCTCTGTAGCAATGCAGCTGACCTCGGGA
  AAGATGCCATGGAAGTGCCTGAAGCCGGGATGATAAGTGACAGTGAGCTGCAGCACATCTCTGATTCTCC
  CATCATCGATGGGCAGCAGCAGTCGGAAACCCCACCCCCCTCAGACATTGCTGACATTGACAACCTGGAG
  CAGGCCGACCAGGAGAGGGAGGTGAAGAGGCGGAAGTACGAGTGCACAATATGTGGACGCAAATTTATCC
  AGAAAAGCCACTGGAGGGAACACATGTACATACACACCGGGAAGCCTTTCAAGTGCAGCACTTGTGACAA
  AAGCTTTTGCAGGGCCAACCAGGCTGCCCGCCACGTGTGCCTCAACCAGAGCATCGACACTTACACCATG
  GTGGACAAACAGACTCTGGAACTCTGCACATTTGAGGAAGGGAGTCAGATGGACAACATGCTGGTGCAAA
  CAAACAAACCCTACAAATGCAACTTGTGTGACAAAACATTCTCCACTCCCAATGAGGTTGTTAAACATTC
  ATGCCAAAACCAGAACTCGGATGTTTTTGCCCTAGACGAAGGGCGATCCATTCTCCTGGGCAGTGGGGAC
  TCGGAAGTAACGGAGCCTGACCACCCAGTGTTAGCTTCCATCAAAAAGGAACAAGAAACCGTCTTACTAG
  AC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204198 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDLANHGLILLQQLNAQREFGFLCDCTVAIGDVYFKAHKSVLASFSNYFKMLFVHQTSECVRLKPTDIQP
  DIFSYLLHLMYTGKMAPQLIDPVRLEQGIKFLHAYPLIQEASLASQGAFSHPDQVFPLASSLYGIQIADH
  QLRQATKIASAPEKLGRDPRPQTSRISQEQVPEASQLSQLTSNLAQVNRTNMTPSDPLQTSLSPELVSTP
  VPPPPPGEETNLEASSSDEQPASLTIAHVKPSIMKRNGSFPKYYACHLCGRRFTLRSSLREHLQIHTGVP
  FTSSQQGESRVPLTLCSNAADLGKDAMEVPEAGMISDSELQHISDSPIIDGQQQSETPPPSDIADIDNLE
  QADQEREVKRRKYECTICGRKFIQKSHWREHMYIHTGKPFKCSTCDKSFCRANQAARHVCLNQSIDTYTM
  VDKQTLELCTFEEGSQMDNMLVQTNKPYKCNLCDKTFSTPNEVVKHSCQNQNSDVFALDEGRSILLGSGD
  SEVTEPDHPVLASIKKEQETVLLD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020861
• ORF Size: 1542 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020861.3
• RefSeq Size: 3105 bp
• RefSeq ORF: 1545 bp
• Locus ID: 57621
• UniProt ID: Q8N680
• Cytogenetics: 6q25.1
• Domains: BTB, zf-C2H2
• Protein Families: Transcription Factors
• MW: 57.3 kDa
• Gene Summary: May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]