CBX6 (NM_014292) Human Tagged ORF Clone

CAT#: RC204166

CBX6 (Myc-DDK-tagged)-Human chromobox homolog 6 (CBX6)

Product Images

Circular map for RC204166

• 

  Western blot validation of overexpression lysate (Cat# LY415379) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204166 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CBX6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204166 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGTCTGCAGTGGGCGAGCGGGTCTTCGCGGCCGAATCCATCATCAAACGGCGGATCCGAAAGG
  GACGCATCGAGTACCTGGTGAAATGGAAGGGGTGGGCGATCAAGTACAGCACTTGGGAGCCCGAGGAGAA
  CATCCTGGACTCGCGGCTCATTGCAGCCTTCGAACAAAAGGAGAGGGAGCGTGAGCTGTATGGGCCCAAG
  AAGAGGGGACCCAAACCCAAAACTTTCCTCCTGAAGGCGCGGGCCCAGGCCGAGGCCCTCCGCATCAGTG
  ATGTGCATTTCTCTGTCAAGCCGAGCGCCAGTGCCTCCTCGCCCAAGCTGCACTCCAGCGCAGCCGTGCA
  CCGGCTCAAGAAGGACATCCGCCGCTGCCACCGTATGTCCCGCCGTCCCCTGCCCCGCCCGGACCCGCAG
  GGGGGCAGCCCCGGACTGCGCCCGCCCATTTCGCCCTTCTCGGAGACGGTGCGCATCATCAACCGCAAGG
  TGAAGCCGCGGGAGCCCAAGCGGAACCGCATCATCCTGAACCTGAAGGTGATCGACAAGGGCGCTGGCGG
  CGGGGGCGCCGGGCAGGGGGCCGGGGCGCTGGCCCGCCCCAAAGTCCCCTCGCGGAACCGCGTTATAGGC
  AAGAGCAAGAAGTTCAGCGAGAGCGTCCTGCGTACACAGATCCGCCACATGAAGTTCGGCGCCTTTGCGC
  TGTACAAGCCTCCGCCCGCCCCCCTGGTAGCCCCGTCCCCCGGCAAGGCTGAGGCCTCAGCCCCGGGCCC
  TGGGCTACTTCTGGCCGCCCCCGCCGCCCCCTACGACGCCCGCAGCTCTGGCTCCTCCGGCTGCCCCTCG
  CCTACACCACAGTCCTCTGACCCCGACGACACGCTCCCCAAGCTCCTCCCCGAGACCGTGAGCCCATCCG
  CCCCCAGCTGGCGCGAGCCGGAGGTGCTCGACCTGTCCCTCCCTCCCGAGTCGGCAGCCACCAGCAAGCG
  GGCACCGCCTGAGGTCACAGCTGCTGCCGGCCCGGCACCTCCCACGGCCCCTGAGCCCGCCGGTGCCTCC
  TCCGAGCCCGAGGCTGGGGACTGGCGCCCCGAGATGTCACCCTGCTCCAATGTGGTCGTCACCGATGTCA
  CCAGCAACCTCCTGACGGTCACAATCAAGGAATTCTGCAACCCTGAGGATTTCGAGAAGGTGGCTGCTGG
  GGTAGCAGGCGCCGCTGGGGGCGGTGGCAGCATTGGGGCGAGCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204166 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MELSAVGERVFAAESIIKRRIRKGRIEYLVKWKGWAIKYSTWEPEENILDSRLIAAFEQKERERELYGPK
  KRGPKPKTFLLKARAQAEALRISDVHFSVKPSASASSPKLHSSAAVHRLKKDIRRCHRMSRRPLPRPDPQ
  GGSPGLRPPISPFSETVRIINRKVKPREPKRNRIILNLKVIDKGAGGGGAGQGAGALARPKVPSRNRVIG
  KSKKFSESVLRTQIRHMKFGAFALYKPPPAPLVAPSPGKAEASAPGPGLLLAAPAAPYDARSSGSSGCPS
  PTPQSSDPDDTLPKLLPETVSPSAPSWREPEVLDLSLPPESAATSKRAPPEVTAAAGPAPPTAPEPAGAS
  SEPEAGDWRPEMSPCSNVVVTDVTSNLLTVTIKEFCNPEDFEKVAAGVAGAAGGGGSIGASK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014292
• ORF Size: 1236 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014292.2
• RefSeq Size: 3284 bp
• RefSeq ORF: 1239 bp
• Locus ID: 23466
• UniProt ID: O95503
• Cytogenetics: 22q13.1
• Protein Families: Transcription Factors
• MW: 43.9 kDa
• Gene Summary: Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development (PubMed:21282530). PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Possibly contributes to the target selectivity of the PRC1 complex by binding specific regions of chromatin (PubMed:18927235). Recruitment to chromatin might occur in an H3K27me3-independent fashion (By similarity). May have a PRC1-independent function in embryonic stem cells (By similarity).[UniProtKB/Swiss-Prot Function]