ETFDH (NM_004453) Human Tagged ORF Clone

CAT#: RC204149

ETFDH (Myc-DDK-tagged)-Human electron-transferring-flavoprotein dehydrogenase (ETFDH), nuclear gene encoding mitochondrial protein

Product Images

Circular map for RC204149

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ETFDH
• Synonyms: ETFQO; MADD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204149 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGTGCCGCTAGCCAAGCTGTCCTGCCTGGCATATCAGTGCTTTCATGCCTTAAAAATTAAGAAAA
  ATTATCTACCTCTATGTGCTATAAGATGGTCTTCAACTTCTACTGTGCCTCGAATTACTACCCATTATAC
  TATTTATCCCCGGGATAAGGACAAGAGATGGGAAGGAGTGAACATGGAAAGGTTTGCAGAAGAAGCAGAT
  GTTGTAATAGTTGGTGCAGGCCCTGCAGGGCTCTCTGCAGCTGTTCGTCTAAAACAGTTGGCTGTGGCAC
  ATGAAAAGGACATCCGTGTGTGTCTAGTGGAGAAAGCTGCCCAGATAGGAGCTCATACTCTCTCAGGGGC
  TTGCCTTGATCCAGGTGCTTTTAAAGAACTCTTCCCAGACTGGAAAGAGAAGGGGGCTCCACTTAACACT
  CCTGTAACAGAAGACAGATTTGGAATTTTAACAGAGAAATACAGAATTCCTGTGCCAATTCTTCCAGGGC
  TTCCAATGAATAATCATGGCAATTACATTGTACGCTTGGGACATTTAGTGAGCTGGATGGGCGAACAAGC
  AGAAGCCCTTGGTGTTGAAGTATACCCTGGTTATGCAGCTGCTGAGGTCCTTTTTCATGATGATGGTAGT
  GTAAAAGGAATTGCCACTAACGATGTAGGGATACAAAAGGATGGTGCACCAAAGGCAACATTTGAGAGAG
  GACTGGAACTACATGCTAAAGTCACAATTTTTGCAGAAGGTTGCCATGGACATCTAGCCAAGCAACTATA
  TAAGAAGTTTGATTTGAGAGCAAATTGTGAACCTCAAACCTACGGGATTGGACTGAAGGAGTTATGGGTT
  ATTGATGAAAAGAACTGGAAACCTGGGAGAGTAGATCACACTGTTGGTTGGCCCTTGGACAGACATACCT
  ATGGAGGATCTTTCCTCTATCATTTGAATGAAGGTGAACCCCTAGTAGCTCTTGGTCTTGTGGTTGGTCT
  AGACTATCAGAATCCATACCTGAGTCCATTTAGAGAGTTCCAAAGGTGGAAACACCATCCTAGCATTCGG
  CCAACCTTGGAAGGTGGAAAAAGGATTGCATACGGAGCCAGAGCTCTCAATGAAGGTGGCTTTCAGTCTA
  TACCAAAACTCACCTTTCCTGGTGGTTTACTAATTGGTTGTAGTCCTGGTTTTATGAATGTTCCCAAGAT
  CAAAGGTACTCACACAGCAATGAAAAGTGGAATTTTAGCAGCAGAATCTATTTTTAATCAACTAACTAGT
  GAAAATCTCCAATCAAAGACAATAGGACTCCATGTAACTGAATATGAGGACAATTTGAAGAACTCATGGG
  TATGGAAAGAGCTATATTCTGTTAGAAATATAAGACCGTCCTGCCACGGAGTACTGGGTGTATATGGAGG
  GATGATTTACACTGGAATCTTTTACTGGATATTGAGAGGAATGGAGCCGTGGACTCTGAAACATAAAGGT
  TCTGACTTTGAACGGCTCAAGCCAGCCAAGGATTGCACACCTATTGAGTATCCAAAACCCGATGGACAGA
  TCAGTTTTGACCTCTTGTCATCTGTGGCTCTGAGTGGTACTAATCATGAACATGACCAGCCGGCACACTT
  AACCTTAAGGGATGACAGTATACCTGTAAATAGAAATCTGTCGATATATGATGGGCCCGAGCAGCGATTC
  TGTCCTGCAGGAGTTTATGAATTTGTACCTGTGGAACAAGGTGATGGATTTCGGTTACAGATAAATGCTC
  AGAACTGTGTACATTGTAAAACATGTGATATTAAAGATCCAAGTCAGAATATTAACTGGGTGGTACCTGA
  AGGTGGAGGAGGACCTGCTTACAATGGAATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204149 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLVPLAKLSCLAYQCFHALKIKKNYLPLCAIRWSSTSTVPRITTHYTIYPRDKDKRWEGVNMERFAEEAD
  VVIVGAGPAGLSAAVRLKQLAVAHEKDIRVCLVEKAAQIGAHTLSGACLDPGAFKELFPDWKEKGAPLNT
  PVTEDRFGILTEKYRIPVPILPGLPMNNHGNYIVRLGHLVSWMGEQAEALGVEVYPGYAAAEVLFHDDGS
  VKGIATNDVGIQKDGAPKATFERGLELHAKVTIFAEGCHGHLAKQLYKKFDLRANCEPQTYGIGLKELWV
  IDEKNWKPGRVDHTVGWPLDRHTYGGSFLYHLNEGEPLVALGLVVGLDYQNPYLSPFREFQRWKHHPSIR
  PTLEGGKRIAYGARALNEGGFQSIPKLTFPGGLLIGCSPGFMNVPKIKGTHTAMKSGILAAESIFNQLTS
  ENLQSKTIGLHVTEYEDNLKNSWVWKELYSVRNIRPSCHGVLGVYGGMIYTGIFYWILRGMEPWTLKHKG
  SDFERLKPAKDCTPIEYPKPDGQISFDLLSSVALSGTNHEHDQPAHLTLRDDSIPVNRNLSIYDGPEQRF
  CPAGVYEFVPVEQGDGFRLQINAQNCVHCKTCDIKDPSQNINWVVPEGGGGPAYNGM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004453
• ORF Size: 1851 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004453.1, NP_004444.1
• RefSeq Size: 2396 bp
• RefSeq ORF: 1854 bp
• Locus ID: 2110
• UniProt ID: Q16134
• Cytogenetics: 4q32.1
• Domains: ETFD
• MW: 68.5 kDa
• Gene Summary: This gene encodes a component of the electron-transfer system in mitochondria and is essential for electron transfer from a number of mitochondrial flavin-containing dehydrogenases to the main respiratory chain. Mutations in this gene are associated with glutaric acidemia. Alternatively spliced transcript variants that encode distinct isoforms have been observed. [provided by RefSeq, Aug 2013]