STK38 (NM_007271) Human Tagged ORF Clone

CAT#: RC204133

STK38 (Myc-DDK-tagged)-Human serine/threonine kinase 38 (STK38)

Product Images

Western blot validation of overexpression lysate (Cat# LY402123) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204133 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified STK38 protein (Cat# TP304133). The protein was produced from HEK293T cells transfected with STK38 cDNA clone (Cat# RC204133) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: STK38
• Synonyms: NDR; NDR1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204133 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAATGACAGGCTCAACACCTTGCTCATCCATGAGTAACCACACAAAGGAAAGGGTGACAATGACCA
  AAGTGACACTGGAGAATTTTTATAGCAACCTTATCGCTCAACATGAAGAACGAGAAATGAGACAAAAGAA
  GTTAGAAAAGGTGATGGAAGAAGAAGGCCTAAAAGATGAGGAGAAACGACTCCGGAGATCAGCACATGCT
  CGGAAGGAAACAGAGTTTCTTCGTTTGAAGAGAACAAGACTTGGATTGGAAGATTTTGAGTCCTTAAAAG
  TAATAGGCAGAGGAGCATTTGGTGAGGTACGGCTTGTTCAGAAGAAAGATACGGGACATGTGTATGCAAT
  GAAAATACTCCGTAAAGCAGATATGCTTGAAAAAGAGCAGGTTGGCCACATTCGTGCGGAGCGTGACATT
  CTAGTGGAGGCAGACAGTTTGTGGGTTGTGAAAATGTTCTATAGTTTTCAGGATAAGCTAAACCTCTACC
  TAATCATGGAGTTCCTGCCTGGAGGGGACATGATGACCTTGTTGATGAAAAAAGACACTCTGACAGAAGA
  GGAGACTCAGTTTTATATAGCAGAAACAGTATTAGCCATAGACTCTATTCACCAACTTGGATTCATCCAC
  AGAGACATCAAACCAGACAACCTTCTTTTGGACAGCAAGGGCCATGTGAAACTTTCTGACTTTGGTCTTT
  GCACAGGACTGAAAAAAGCACATAGGACAGAATTTTATAGGAATCTGAACCACAGCCTCCCCAGTGATTT
  CACTTTCCAGAACATGAATTCCAAAAGGAAAGCAGAAACCTGGAAAAGAAATAGACGTCAGCTAGCCTTC
  TCCACAGTAGGCACTCCTGACTACATTGCTCCTGAGGTGTTCATGCAGACCGGGTACAACAAGCTCTGTG
  ATTGGTGGTCGCTTGGGGTGATCATGTATGAGATGCTCATCGGCTACCCACCTTTCTGTTCTGAGACCCC
  TCAAGAGACATATAAGAAGGTGATGAACTGGAAAGAAACTTTGACTTTTCCTCCAGAAGTTCCCATCTCT
  GAGAAAGCCAAGGATCTAATTTTGAGGTTCTGCTGTGAATGGGAACATAGAATTGGAGCTCCTGGAGTTG
  AGGAAATAAAAAGTAACTCTTTTTTTGAAGGCGTTGACTGGGAACATATCAGAGAGAGACCTGCTGCAAT
  ATCTATTGAAATCAAAAGCATTGATGATACCTCAAACTTCGATGAGTTTCCAGAATCTGATATTCTTAAG
  CCAACAGTGGCCACAAGTAATCATCCTGAGACTGACTACAAGAACAAAGACTGGGTCTTCATCAATTACA
  CGTACAAGCGCTTTGAGGGCCTGACTGCAAGGGGGGCAATACCTTCCTACATGAAAGCAGCAAAA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204133 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAMTGSTPCSSMSNHTKERVTMTKVTLENFYSNLIAQHEEREMRQKKLEKVMEEEGLKDEEKRLRRSAHA
  RKETEFLRLKRTRLGLEDFESLKVIGRGAFGEVRLVQKKDTGHVYAMKILRKADMLEKEQVGHIRAERDI
  LVEADSLWVVKMFYSFQDKLNLYLIMEFLPGGDMMTLLMKKDTLTEEETQFYIAETVLAIDSIHQLGFIH
  RDIKPDNLLLDSKGHVKLSDFGLCTGLKKAHRTEFYRNLNHSLPSDFTFQNMNSKRKAETWKRNRRQLAF
  STVGTPDYIAPEVFMQTGYNKLCDWWSLGVIMYEMLIGYPPFCSETPQETYKKVMNWKETLTFPPEVPIS
  EKAKDLILRFCCEWEHRIGAPGVEEIKSNSFFEGVDWEHIRERPAAISIEIKSIDDTSNFDEFPESDILK
  PTVATSNHPETDYKNKDWVFINYTYKRFEGLTARGAIPSYMKAAK
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007271
• ORF Size: 1395 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007271.4
• RefSeq Size: 3593 bp
• RefSeq ORF: 1398 bp
• Locus ID: 11329
• UniProt ID: Q15208
• Cytogenetics: 6p21.31
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• MW: 54.2 kDa
• Gene Summary: This gene encodes a member of the AGC serine/threonine kinase family of proteins. The kinase activity of this protein is regulated by autophosphorylation and phosphorylation by other upstream kinases. This protein has been shown to function in the cell cycle and apoptosis. This protein has also been found to regulate the protein stability and transcriptional activity of the MYC oncogene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2015]