COMT (NM_007310) Human Tagged ORF Clone
COMT (Myc-DDK-tagged)-Human catechol-O-methyltransferase (COMT), transcript variant 4
|Product Name||COMT (NM_007310) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC204127 representing NM_007310
Red=Cloning site Blue=ORF Green=Tags(s)
>RC204127 representing NM_007310
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||663 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_007310.1, NM_007310.2, NP_009294.1|
|Protein Families||Druggable Genome, Transmembrane|
|Protein Pathways||Metabolic pathways, Tyrosine metabolism|
|Gene Summary||Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. [provided by RefSeq, Sep 2008]|
|The use of this cDNA Clones has been cited in the following citations:|
Identification of Potential Off-target Toxicity Liabilities of Catechol-O-methyltransferase Inhibitors by Differential Competition Capture Compound Mass Spectrometry
,von Kleist, L;Michaelis, S;Bartho, K;Graebner, O;Schlief, M;Dreger, M;Schrey, AK;Sefkow, M;Kroll, F;Koester, H;Luo, Y;,
J. Med. Chem. Apr 2016
,PubMed ID 27074629
|cDNA Clone Resources|
|SC109094||COMT (untagged)-Human catechol-O-methyltransferase (COMT), transcript variant 4||
|SC320911||COMT (untagged)-Human catechol-O-methyltransferase (COMT), transcript variant 4||
|RG204127||COMT (GFP-tagged) - Human catechol-O-methyltransferase (COMT), transcript variant 4||
|RC204127L3||Lenti-ORF clone of COMT (Myc-DDK-tagged)-Human catechol-O-methyltransferase (COMT), transcript variant 4||
|RC204127L4||Lenti-ORF clone of COMT (mGFP-tagged)-Human catechol-O-methyltransferase (COMT), transcript variant 4||