PTCD3 (NM_017952) Human Tagged ORF Clone

CAT#: RC204119

PTCD3 (Myc-DDK-tagged)-Human Pentatricopeptide repeat domain 3 (PTCD3), nuclear gene encoding mitochondrial protein

Product Images

Circular map for RC204119

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  Western blot validation of overexpression lysate (Cat# LY402633) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204119 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PTCD3 protein (Cat# TP304119). The protein was produced from HEK293T cells transfected with PTCD3 cDNA clone (Cat# RC204119) using MegaTran 2.0 (Cat# TT210002).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PTCD3 (Cat# RC204119, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTCD3(Cat# TA810262). Positive lysates LY402633 (100ug) and LC402633 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PTCD3
• Synonyms: MRP-S39
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204119 representing NM_017952
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTTGTATCTGCTGTTCGCTGGCTGGGCCTCCGCAGCAGGCTTGGCCAGCCGCTGACGGGTCGGC
  GGGCGGGTTTGTGTGAACAGGCACGCAGCTGCAGATTTTATTCTGGTAGTGCAACCCTCTCAAAGGTTGA
  AGGAACTGATGTAACAGGGATTGAAGAAGTAGTAATTCCAAAAAAGAAAACTTGGGATAAAGTAGCCGTT
  CTTCAGGCACTTGCATCCACAGTAAACAGGGATACCACAGCTGTGCCTTATGTGTTTCAAGATGATCCTT
  ACCTTATGCCAGCATCATCTTTGGAATCTCGTTCATTTTTACTGGCAAAGAAATCCGGGGAGAATGTGGC
  CAAGTTTATTATTAATTCATACCCCAAATATTTTCAGAAGGACATAGCTGAACCTCATATACCGTGTTTA
  ATGCCTGAGTACTTTGAACCTCAGATCAAAGACATAAGTGAAGCCGCCCTGAAGGAACGAATTGAGCTCA
  GAAAAGTCAAAGCCTCTGTGGACATGTTTGATCAGCTTTTGCAAGCAGGAACCACTGTGTCTCTTGAAAC
  AACAAATAGTCTCTTGGATTTATTGTGTTACTATGGTGACCAGGAGCCCTCAACTGATTACCATTTTCAA
  CAAACTGGACAGTCAGAAGCATTGGAAGAGGAAAATGATGAGACATCTAGGAGGAAAGCTGGTCATCAGT
  TTGGAGTTACATGGCGAGCAAAAAACAACGCTGAGAGAATCTTTTCTCTAATGCCAGAGAAAAATGAACA
  TTCCTATTGCACAATGATCCGAGGAATGGTGAAGCACCGAGCTTATGAGCAGGCATTAAACTTGTACACT
  GAGTTACTAAACAACAGACTCCATGCTGATGTATACACATTTAATGCATTGATTGAAGCAACAGTATGTG
  CGATAAATGAGAAATTTGAGGAAAAATGGAGTAAAATACTGGAGCTGCTAAGACACATGGTTGCACAGAA
  GGTGAAACCAAATCTTCAGACTTTTAATACCATTCTGAAATGTCTCCGAAGATTTCATGTGTTTGCAAGA
  TCGCCAGCCTTACAGGTTTTACGTGAAATGAAAGCCATTGGAATAGAACCCTCGCTTGCAACATATCACC
  ATATTATTCGCCTGTTTGATCAACCTGGAGACCCTTTAAAGAGATCATCCTTCATCATTTATGATATAAT
  GAATGAATTAATGGGAAAGAGATTTTCTCCAAAGGACCCGGATGATGATAAGTTTTTTCAGTCAGCCATG
  AGCATATGCTCATCTCTCAGAGATCTAGAACTTGCCTACCAAGTACATGGCCTTTTAAAAACCGGAGACA
  ACTGGAAATTCATTGGACCTGATCAACATCGTAATTTCTATTATTCCAAGTTCTTCGATTTGATTTGTCT
  AATGGAACAAATTGATGTTACCTTGAAGTGGTATGAGGACCTGATACCTTCAGCCTACTTTCCCCACTCC
  CAAACAATGATACATCTTCTCCAAGCATTGGATGTGGCCAATCGGCTAGAAGTGATTCCTAAAATTTGGA
  AAGATAGTAAAGAATATGGTCATACTTTCCGCAGTGACCTGAGAGAAGAGATCCTGATGCTCATGGCAAG
  GGACAAGCACCCACCAGAGCTTCAGGTGGCATTTGCTGACTGTGCTGCTGATATCAAATCTGCGTATGAA
  AGCCAACCCATCAGACAGACTGCTCAGGATTGGCCAGCCACCTCTCTCAACTGTATAGCTATCCTCTTTT
  TAAGGGCTGGGAGAACTCAGGAAGCCTGGAAAATGTTGGGGCTTTTCAGGAAGCATAATAAGATTCCTAG
  AAGTGAGTTGCTGAATGAGCTTATGGACAGTGCAAAAGTGTCTAACAGCCCTTCCCAGGCCATTGAAGTA
  GTAGAGCTGGCAAGTGCCTTCAGCTTACCTATTTGTGAGGGCCTCACCCAGAGAGTAATGAGTGATTTTG
  CAATCAACCAGGAACAAAAGGAAGCCCTAAGTAATCTAACTGCATTGACCAGTGACAGTGATACTGACAG
  CAGCAGTGACAGCGACAGTGACACCAGTGAAGGCAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204119 representing NM_017952
  Red=Cloning site Green=Tags(s)
  
  MAVVSAVRWLGLRSRLGQPLTGRRAGLCEQARSCRFYSGSATLSKVEGTDVTGIEEVVIPKKKTWDKVAV
  LQALASTVNRDTTAVPYVFQDDPYLMPASSLESRSFLLAKKSGENVAKFIINSYPKYFQKDIAEPHIPCL
  MPEYFEPQIKDISEAALKERIELRKVKASVDMFDQLLQAGTTVSLETTNSLLDLLCYYGDQEPSTDYHFQ
  QTGQSEALEEENDETSRRKAGHQFGVTWRAKNNAERIFSLMPEKNEHSYCTMIRGMVKHRAYEQALNLYT
  ELLNNRLHADVYTFNALIEATVCAINEKFEEKWSKILELLRHMVAQKVKPNLQTFNTILKCLRRFHVFAR
  SPALQVLREMKAIGIEPSLATYHHIIRLFDQPGDPLKRSSFIIYDIMNELMGKRFSPKDPDDDKFFQSAM
  SICSSLRDLELAYQVHGLLKTGDNWKFIGPDQHRNFYYSKFFDLICLMEQIDVTLKWYEDLIPSAYFPHS
  QTMIHLLQALDVANRLEVIPKIWKDSKEYGHTFRSDLREEILMLMARDKHPPELQVAFADCAADIKSAYE
  SQPIRQTAQDWPATSLNCIAILFLRAGRTQEAWKMLGLFRKHNKIPRSELLNELMDSAKVSNSPSQAIEV
  VELASAFSLPICEGLTQRVMSDFAINQEQKEALSNLTALTSDSDTDSSSDSDSDTSEGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017952
• ORF Size: 2067 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017952.6
• RefSeq Size: 6708 bp
• RefSeq ORF: 2070 bp
• Locus ID: 55037
• UniProt ID: Q96EY7
• Cytogenetics: 2p11.2
• Domains: PPR
• MW: 78.4 kDa
• Gene Summary: Mitochondrial RNA-binding protein that has a role in mitochondrial translation.[UniProtKB/Swiss-Prot Function]