PTCD3 (NM_017952) Human Tagged ORF Clone

CAT#: RC204119

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PTCD3 (Myc-DDK-tagged)-Human Pentatricopeptide repeat domain 3 (PTCD3), nuclear gene encoding mitochondrial protein



Reconstitution Protocol

USD 631.00

In Stock*

Size
    • 10 ug

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Frequently bought together (4)
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PTCD3
Synonyms MRP-S39
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC204119 representing NM_017952
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGTTGTATCTGCTGTTCGCTGGCTGGGCCTCCGCAGCAGGCTTGGCCAGCCGCTGACGGGTCGGC
GGGCGGGTTTGTGTGAACAGGCACGCAGCTGCAGATTTTATTCTGGTAGTGCAACCCTCTCAAAGGTTGA
AGGAACTGATGTAACAGGGATTGAAGAAGTAGTAATTCCAAAAAAGAAAACTTGGGATAAAGTAGCCGTT
CTTCAGGCACTTGCATCCACAGTAAACAGGGATACCACAGCTGTGCCTTATGTGTTTCAAGATGATCCTT
ACCTTATGCCAGCATCATCTTTGGAATCTCGTTCATTTTTACTGGCAAAGAAATCCGGGGAGAATGTGGC
CAAGTTTATTATTAATTCATACCCCAAATATTTTCAGAAGGACATAGCTGAACCTCATATACCGTGTTTA
ATGCCTGAGTACTTTGAACCTCAGATCAAAGACATAAGTGAAGCCGCCCTGAAGGAACGAATTGAGCTCA
GAAAAGTCAAAGCCTCTGTGGACATGTTTGATCAGCTTTTGCAAGCAGGAACCACTGTGTCTCTTGAAAC
AACAAATAGTCTCTTGGATTTATTGTGTTACTATGGTGACCAGGAGCCCTCAACTGATTACCATTTTCAA
CAAACTGGACAGTCAGAAGCATTGGAAGAGGAAAATGATGAGACATCTAGGAGGAAAGCTGGTCATCAGT
TTGGAGTTACATGGCGAGCAAAAAACAACGCTGAGAGAATCTTTTCTCTAATGCCAGAGAAAAATGAACA
TTCCTATTGCACAATGATCCGAGGAATGGTGAAGCACCGAGCTTATGAGCAGGCATTAAACTTGTACACT
GAGTTACTAAACAACAGACTCCATGCTGATGTATACACATTTAATGCATTGATTGAAGCAACAGTATGTG
CGATAAATGAGAAATTTGAGGAAAAATGGAGTAAAATACTGGAGCTGCTAAGACACATGGTTGCACAGAA
GGTGAAACCAAATCTTCAGACTTTTAATACCATTCTGAAATGTCTCCGAAGATTTCATGTGTTTGCAAGA
TCGCCAGCCTTACAGGTTTTACGTGAAATGAAAGCCATTGGAATAGAACCCTCGCTTGCAACATATCACC
ATATTATTCGCCTGTTTGATCAACCTGGAGACCCTTTAAAGAGATCATCCTTCATCATTTATGATATAAT
GAATGAATTAATGGGAAAGAGATTTTCTCCAAAGGACCCGGATGATGATAAGTTTTTTCAGTCAGCCATG
AGCATATGCTCATCTCTCAGAGATCTAGAACTTGCCTACCAAGTACATGGCCTTTTAAAAACCGGAGACA
ACTGGAAATTCATTGGACCTGATCAACATCGTAATTTCTATTATTCCAAGTTCTTCGATTTGATTTGTCT
AATGGAACAAATTGATGTTACCTTGAAGTGGTATGAGGACCTGATACCTTCAGCCTACTTTCCCCACTCC
CAAACAATGATACATCTTCTCCAAGCATTGGATGTGGCCAATCGGCTAGAAGTGATTCCTAAAATTTGGA
AAGATAGTAAAGAATATGGTCATACTTTCCGCAGTGACCTGAGAGAAGAGATCCTGATGCTCATGGCAAG
GGACAAGCACCCACCAGAGCTTCAGGTGGCATTTGCTGACTGTGCTGCTGATATCAAATCTGCGTATGAA
AGCCAACCCATCAGACAGACTGCTCAGGATTGGCCAGCCACCTCTCTCAACTGTATAGCTATCCTCTTTT
TAAGGGCTGGGAGAACTCAGGAAGCCTGGAAAATGTTGGGGCTTTTCAGGAAGCATAATAAGATTCCTAG
AAGTGAGTTGCTGAATGAGCTTATGGACAGTGCAAAAGTGTCTAACAGCCCTTCCCAGGCCATTGAAGTA
GTAGAGCTGGCAAGTGCCTTCAGCTTACCTATTTGTGAGGGCCTCACCCAGAGAGTAATGAGTGATTTTG
CAATCAACCAGGAACAAAAGGAAGCCCTAAGTAATCTAACTGCATTGACCAGTGACAGTGATACTGACAG
CAGCAGTGACAGCGACAGTGACACCAGTGAAGGCAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC204119 representing NM_017952
Red=Cloning site Green=Tags(s)

MAVVSAVRWLGLRSRLGQPLTGRRAGLCEQARSCRFYSGSATLSKVEGTDVTGIEEVVIPKKKTWDKVAV
LQALASTVNRDTTAVPYVFQDDPYLMPASSLESRSFLLAKKSGENVAKFIINSYPKYFQKDIAEPHIPCL
MPEYFEPQIKDISEAALKERIELRKVKASVDMFDQLLQAGTTVSLETTNSLLDLLCYYGDQEPSTDYHFQ
QTGQSEALEEENDETSRRKAGHQFGVTWRAKNNAERIFSLMPEKNEHSYCTMIRGMVKHRAYEQALNLYT
ELLNNRLHADVYTFNALIEATVCAINEKFEEKWSKILELLRHMVAQKVKPNLQTFNTILKCLRRFHVFAR
SPALQVLREMKAIGIEPSLATYHHIIRLFDQPGDPLKRSSFIIYDIMNELMGKRFSPKDPDDDKFFQSAM
SICSSLRDLELAYQVHGLLKTGDNWKFIGPDQHRNFYYSKFFDLICLMEQIDVTLKWYEDLIPSAYFPHS
QTMIHLLQALDVANRLEVIPKIWKDSKEYGHTFRSDLREEILMLMARDKHPPELQVAFADCAADIKSAYE
SQPIRQTAQDWPATSLNCIAILFLRAGRTQEAWKMLGLFRKHNKIPRSELLNELMDSAKVSNSPSQAIEV
VELASAFSLPICEGLTQRVMSDFAINQEQKEALSNLTALTSDSDTDSSSDSDSDTSEGK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_017952
ORF Size 2067 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_017952.6
RefSeq Size 6708 bp
RefSeq ORF 2070 bp
Locus ID 55037
UniProt ID Q96EY7
Cytogenetics 2p11.2
Domains PPR
MW 78.4 kDa
Gene Summary Mitochondrial RNA-binding protein that has a role in mitochondrial translation.[UniProtKB/Swiss-Prot Function]
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