HNRPDL (HNRNPDL) (NM_031372) Human Tagged ORF Clone

CAT#: RC204064

HNRPDL (Myc-DDK-tagged)-Human heterogeneous nuclear ribonucleoprotein D-like (HNRPDL), transcript variant 2

Product Images

Circular map for RC204064

• 

  Western blot validation of overexpression lysate (Cat# LY410550) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204064 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HNRPDL
• Synonyms: HNRNP; HNRPDL; JKTBP; JKTBP2; laAUF1; LGMD1G; LGMDD3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204064 representing NM_031372
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGTCCCGCCCAGGCTTTCCCATGTGCCGCCGCCATTGTTCCCCTCCGCTCCCGCTACTTTAGCCT
  CCCGCAGCCTCTCCCATTGGCGGCCGCGGCCGCCGCGGCAGCTAGCCCCGCTCCTCCCTTCGCTCGCTCC
  CAGCTCCGCCCGGCAGGGGGCGCGCCGGGCCCAGCGCCACGTCACCGCCCAGCAGCCCTCCCGATTGGCG
  GGCGGGGCGGCTATAAAGGGAGGGCGCAGGCGGCGCCCGGATCTCTTCCGCCGCCATTTTAAATCCAGCT
  CCATACAACGCTCCGCCGCCGCTGCTGCCGCGACCCGGACTGCGCGCCAGCACCCCCCTGCCGACAGCTC
  CGTCACTATGGAGGATATGAACGAGTACAGCAATATAGAGGAATTCGCAGAGGGATCCAAGATCAACGCG
  AGCAAGAATCAGCAGGATGACGGTAAAATGTTTATTGGAGGCTTGAGCTGGGATACAAGCAAAAAAGATC
  TGACAGAGTACTTGTCTCGATTTGGGGAAGTTGTAGACTGCACAATTAAAACAGATCCAGTCACTGGGAG
  ATCAAGAGGATTTGGATTTGTGCTTTTCAAAGATGCTGCTAGTGTTGATAAGGTTTTGGAACTGAAAGAA
  CACAAACTGGATGGCAAATTGATAGATCCCAAAAGGGCCAAAGCTTTAAAAGGGAAAGAACCTCCCAAAA
  AGGTTTTTGTGGGTGGATTGAGCCCGGATACTTCTGAAGAACAAATTAAAGAATATTTTGGAGCCTTTGG
  AGAGATTGAAAATATTGAACTTCCCATGGATACAAAAACAAATGAAAGAAGAGGATTTTGTTTTATCACA
  TATACTGATGAAGAGCCAGTAAAAAAATTGTTAGAAAGCAGATACCATCAAATTGGTTCTGGGAAGTGTG
  AAATCAAAGTTGCACAACCCAAAGAGGTATATAGGCAGCAACAGCAACAACAAAAAGGTGGAAGAGGTGC
  TGCAGCTGGTGGACGAGGTGGTACGAGGGGTCGTGGCCGAGGTCAGGGCCAAAACTGGAACCAAGGATTT
  AATAACTATTATGATCAAGGATATGGAAATTACAATAGTGCCTATGGTGGTGATCAAAACTATAGTGGCT
  ATGGCGGATATGATTATACTGGGTATAACTATGGGAACTATGGATATGGACAGGGATATGCAGACTACAG
  TGGCCAACAGAGCACTTATGGCAAGGCATCTCGAGGGGGTGGCAATCACCAAAACAATTACCAGCCATAC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204064 representing NM_031372
  Red=Cloning site Green=Tags(s)
  
  MEVPPRLSHVPPPLFPSAPATLASRSLSHWRPRPPRQLAPLLPSLAPSSARQGARRAQRHVTAQQPSRLA
  GGAAIKGGRRRRPDLFRRHFKSSSIQRSAAAAAATRTARQHPPADSSVTMEDMNEYSNIEEFAEGSKINA
  SKNQQDDGKMFIGGLSWDTSKKDLTEYLSRFGEVVDCTIKTDPVTGRSRGFGFVLFKDAASVDKVLELKE
  HKLDGKLIDPKRAKALKGKEPPKKVFVGGLSPDTSEEQIKEYFGAFGEIENIELPMDTKTNERRGFCFIT
  YTDEEPVKKLLESRYHQIGSGKCEIKVAQPKEVYRQQQQQQKGGRGAAAGGRGGTRGRGRGQGQNWNQGF
  NNYYDQGYGNYNSAYGGDQNYSGYGGYDYTGYNYGNYGYGQGYADYSGQQSTYGKASRGGGNHQNNYQPY
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031372
• ORF Size: 1260 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031372.3
• RefSeq Size: 3510 bp
• RefSeq ORF: 1263 bp
• Locus ID: 9987
• UniProt ID: O14979
• Cytogenetics: 4q21.22
• Domains: RRM
• MW: 46.3 kDa
• Gene Summary: This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two RRM domains that bind to RNAs. Three alternatively spliced transcript variants have been described for this gene. One of the variants is probably not translated because the transcript is a candidate for nonsense-mediated mRNA decay. The protein isoforms encoded by this gene are similar to its family member HNRPD. [provided by RefSeq, May 2011]