NRBF2 (NM_030759) Human Tagged ORF Clone

CAT#: RC204061

NRBF2 (Myc-DDK-tagged)-Human nuclear receptor binding factor 2 (NRBF2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NRBF2 (Cat# RC204061, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NRBF2(Cat# TA803856). Positive lysates LY410731 (100ug) and LC410731 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY410731) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204061 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified NRBF2 protein (Cat# TP304061). The protein was produced from HEK293T cells transfected with NRBF2 cDNA clone (Cat# RC204061) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NRBF2
• Synonyms: COPR; COPR1; COPR2; NRBF-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204061 representing NM_030759
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGTAATGGAAGGACCCCTCAACCTGGCTCATCAACAGAGCAGACGAGCAGACCGTTTATTAGCTG
  CAGGCAAATACGAAGAGGCTATTTCTTGTCACAAAAAGGCTGCAGCATATCTTTCTGAAGCCATGAAGCT
  GACACAGTCAGAGCAGGCTCATCTTTCACTGGAATTGCAAAGGGATAGCCATATGAAACAGCTCCTCCTC
  ATCCAAGAGAGATGGAAAAGGGCCCAGCGTGAAGAAAGATTGAAAGCCCAGCAGAACACAGACAAGGATG
  CAGCTGCCCATCTTCAGACATCTCACAAACCCTCTGCAGAGGATGCAGAGGGCCAGAGTCCCCTTTCTCA
  GAAGTACAGCCCTTCCACAGAGAAATGCCTGCCTGAGATTCAGGGGATCTTTGACAGGGATCCAGACACA
  CTACTTTATTTACTTCAGCAAAAGAGTGAGCCAGCAGAGCCATGTATTGGAAGCAAAGCCCCAAAAGATG
  ATAAAACAATTATAGAGGAGCAGGCAACCAAAATTGCAGATTTGAAGAGGCATGTGGAATTCCTTGTGGC
  TGAGAATGAAAGATTAAGGAAAGAAAATAAACAACTAAAGGCTGAAAAGGCCAGACTTCTAAAAGGTCCA
  ATAGAAAAGGAGCTGGATGTAGATGCTGATTTTGTAGAAACGTCAGAGTTATGGAGCTTGCCACCACATG
  CAGAAACTGCTACAGCCTCCTCAACCTGGCAGAAGTTCGCAGCAAATACTGGGAAAGCCAAGGACATTCC
  AATCCCCAATCTTCCTCCCTTGGATTTTCCATCTCCAGAACTTCCTCTTATGGAGCTCTCTGAGGATATT
  CTGAAAGGATTTATGAATAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204061 representing NM_030759
  Red=Cloning site Green=Tags(s)
  
  MEVMEGPLNLAHQQSRRADRLLAAGKYEEAISCHKKAAAYLSEAMKLTQSEQAHLSLELQRDSHMKQLLL
  IQERWKRAQREERLKAQQNTDKDAAAHLQTSHKPSAEDAEGQSPLSQKYSPSTEKCLPEIQGIFDRDPDT
  LLYLLQQKSEPAEPCIGSKAPKDDKTIIEEQATKIADLKRHVEFLVAENERLRKENKQLKAEKARLLKGP
  IEKELDVDADFVETSELWSLPPHAETATASSTWQKFAANTGKAKDIPIPNLPPLDFPSPELPLMELSEDI
  LKGFMNN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030759
• ORF Size: 861 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030759.5
• RefSeq Size: 1866 bp
• RefSeq ORF: 864 bp
• Locus ID: 29982
• UniProt ID: Q96F24
• Cytogenetics: 10q21.3
• Protein Families: Druggable Genome
• MW: 32.2 kDa
• Gene Summary: May modulate transcriptional activation by target nuclear receptors. Can act as transcriptional activator (in vitro).[UniProtKB/Swiss-Prot Function]