WBSCR22 (BUD23) (NM_017528) Human Tagged ORF Clone
Lenti ORF clone of Human Williams Beuren syndrome chromosome region 22 (WBSCR22), transcript variant 2, Myc-DDK-tagged
|Product Name||WBSCR22 (BUD23) (NM_017528) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||HASJ4442; HUSSY-3; MERM1; PP3381; WBMT; WBSCR22|
|E. coli Selection||Chloramphenicol (34 ug/mL)|
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene|
|ORF Size||843 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||1256 bp|
|RefSeq ORF||846 bp|
|Protein Families||Druggable Genome|
|Protein Pathways||Androgen and estrogen metabolism, Histidine metabolism, Selenoamino acid metabolism, Tyrosine metabolism|
|Gene Summary||This gene encodes a protein containing a nuclear localization signal and an S-adenosyl-L-methionine binding motif typical of methyltransferases, suggesting that the encoded protein may act on DNA methylation. This gene is deleted in Williams syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. Alternatively spliced transcript variants have been found. [provided by RefSeq, Feb 2011]|
|cDNA Clone Resources|
|SC127812||WBSCR22 (untagged)-Human Williams Beuren syndrome chromosome region 22 (WBSCR22), transcript variant 2||
|SC320894||WBSCR22 (untagged)-Human Williams Beuren syndrome chromosome region 22 (WBSCR22), transcript variant 2||
|RC204043||WBSCR22 (Myc-DDK-tagged)-Human Williams Beuren syndrome chromosome region 22 (WBSCR22), transcript variant 2||
|RG204043||WBSCR22 (GFP-tagged) - Human Williams Beuren syndrome chromosome region 22 (WBSCR22), transcript variant 2||
|RC204043L4||Lenti ORF clone of Human Williams Beuren syndrome chromosome region 22 (WBSCR22), transcript variant 2, mGFP tagged||