NEU4 (NM_080741) Human Tagged ORF Clone

CAT#: RC203948

NEU4 (Myc-DDK-tagged)-Human sialidase 4 (NEU4), transcript variant 1

Product Images

Circular map for RC203948

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  Western blot validation of overexpression lysate (Cat# LY409058) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203948 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified NEU4 protein (Cat# TP303948). The protein was produced from HEK293T cells transfected with NEU4 cDNA clone (Cat# RC203948) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NEU4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203948 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGAGCTCTGCAGCCTTCCCAAGGTGGCTGAGCATGGGGGTCCCTCGTACCCCTTCACGGACAGTGC
  TCTTCGAGCGGGAGAGGACGGGCCTGACCTACCGCGTGCCCTCGCTGCTCCCCGTGCCCCCCGGGCCCAC
  CCTGCTGGCCTTTGTGGAGCAGCGGCTCAGCCCTGACGACTCCCACGCCCACCGCCTGGTGCTGAGGAGG
  GGCACGCTGGCCGGGGGCTCCGTGCGGTGGGGTGCCCTGCACGTGCTGGGGACAGCAGCCCTGGCGGAGC
  ACCGGTCCATGAACCCCTGCCCTGTGCACGATGCTGGCACGGGCACCGTCTTCCTCTTCTTCATCGCGGT
  GCTGGGCCACACGCCTGAGGCCGTGCAGATCGCCACGGGAAGGAACGCCGCGCGCCTCTGCTGTGTGGCC
  AGCCGTGACGCCGGCCTCTCGTGGGGCAGCGCCCGGGACCTCACCGAGGAGGCCATCGGTGGTGCCGTGC
  AGGACTGGGCCACATTCGCTGTGGGTCCCGGCCACGGCGTGCAGCTGCCCTCAGGCCGCCTGCTGGTACC
  CGCCTACACCTACCGCGTGGACCGCCGAGAGTGTTTTGGCAAGATCTGCCGGACCAGCCCTCACTCCTTC
  GCCTTCTACAGCGATGACCACGGCCGCACCTGGCGCTGTGGAGGCCTCGTGCCCAACCTGCGCTCAGGCG
  AGTGCCAGCTGGCGGCGGTGGACGGTGGGCAGGCCGGCAGCTTCCTCTACTGCAATGCCCGGAGCCCACT
  GGGCAGCCGTGTGCAGGCGCTCAGCACTGACGAGGGCACCTCCTTCCTGCCCGCAGAGCGCGTGGCTTCC
  CTGCCCGAGACTGCCTGGGGCTGCCAGGGCAGCATCGTGGGCTTCCCAGCCCCCGCCCCCAACAGGCCAC
  GGGATGACAGTTGGTCAGTGGGCCCCGGGAGTCCCCTCCAGCCTCCACTCCTCGGTCCTGGAGTCCACGA
  ACCCCCAGAGGAGGCTGCTGTAGACCCCCGTGGAGGCCAGGTGCCTGGTGGGCCCTTCAGCCGTCTGCAG
  CCTCGGGGGGATGGCCCCAGGCAGCCTGGCCCCAGGCCTGGGGTCAGTGGGGATGTGGGGTCCTGGACCC
  TGGCACTCCCCATGCCCTTTGCTGCCCCGCCCCAGAGCCCCACGTGGCTGCTGTACTCCCACCCAGTGGG
  GCGCAGGGCTCGGCTACACATGGGTATCCGCCTGAGCCAGTCCCCGCTGGACCCGCGCAGCTGGACAGAG
  CCCTGGGTGATCTACGAGGGCCCCAGCGGCTACTCCGACCTGGCGTCCATCGGGCCGGCTCCTGAGGGGG
  GCCTGGTTTTTGCCTGCCTGTACGAGAGCGGGGCCAGGACCTCCTATGATGAGATTTCCTTTTGTACATT
  CTCCCTGCGTGAGGTCCTGGAGAACGTGCCCGCCAGCCCCAAGCCGCCCAACCTTGGGGACAAGCCTCGG
  GGGTGCTGCTGGCCCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203948 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MMSSAAFPRWLSMGVPRTPSRTVLFERERTGLTYRVPSLLPVPPGPTLLAFVEQRLSPDDSHAHRLVLRR
  GTLAGGSVRWGALHVLGTAALAEHRSMNPCPVHDAGTGTVFLFFIAVLGHTPEAVQIATGRNAARLCCVA
  SRDAGLSWGSARDLTEEAIGGAVQDWATFAVGPGHGVQLPSGRLLVPAYTYRVDRRECFGKICRTSPHSF
  AFYSDDHGRTWRCGGLVPNLRSGECQLAAVDGGQAGSFLYCNARSPLGSRVQALSTDEGTSFLPAERVAS
  LPETAWGCQGSIVGFPAPAPNRPRDDSWSVGPGSPLQPPLLGPGVHEPPEEAAVDPRGGQVPGGPFSRLQ
  PRGDGPRQPGPRPGVSGDVGSWTLALPMPFAAPPQSPTWLLYSHPVGRRARLHMGIRLSQSPLDPRSWTE
  PWVIYEGPSGYSDLASIGPAPEGGLVFACLYESGARTSYDEISFCTFSLREVLENVPASPKPPNLGDKPR
  GCCWPS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080741
• ORF Size: 1488 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080741.2
• RefSeq Size: 2373 bp
• RefSeq ORF: 1491 bp
• Locus ID: 129807
• UniProt ID: Q8WWR8
• Cytogenetics: 2q37.3
• Protein Pathways: Other glycan degradation, Sphingolipid metabolism
• MW: 52.9 kDa
• Gene Summary: The protein encoded by this gene belongs to a family of glycohydrolytic enzymes, which remove terminal sialic acid residues from various sialo derivatives, such as glycoproteins, glycolipids, oligosaccharides, and gangliosides. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Nov 2009]