PUS7 (NM_019042) Human Tagged ORF Clone

CAT#: RC203902

PUS7 (Myc-DDK-tagged)-Human pseudouridylate synthase 7 homolog (S. cerevisiae) (PUS7)

Product Images

Circular map for RC203902

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PUS7 (Cat# RC203902, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PUS7(Cat# TA501816). Positive lysates LY412749 (100ug) and LC412749 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY412749) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203902 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PUS7 protein (Cat# TP303902). The protein was produced from HEK293T cells transfected with PUS7 cDNA clone (Cat# RC203902) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PUS7
• Synonyms: IDDABS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203902 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGATGACAGAAATGACTGGTGTGTCGCTGAAACGTGGGGCACTGGTTGTCGAAGATAATGACAGTG
  GAGTCCCAGTTGAAGAGACAAAAAAACAGAAGCTGTCGGAATGCAGCCTAACCAAAGGTCAAGATGGGCT
  ACAGAATGACTTTCTGTCCATCAGTGAAGACGTGCCTCGGCCTCCTGACACTGTCAGTACTGGGAAAGGT
  GGAAAGAATTCTGAGGCTCAGTTGGAAGATGAGGAAGAAGAGGAGGAAGATGGACTTTCAGAGGAGTGCG
  AGGAGGAGGAATCAGAGAGTTTTGCAGACATGATGAAGCATGGACTCACTGAGGCTGACGTAGGCATCAC
  CAAGTTTGTGAGTTCTCATCAAGGGTTCTCGGGAATCTTAAAAGAAAGATACTCCGACTTCGTTGTTCAT
  GAAATAGGAAAAGATGGACGGATCAGCCATTTGAATGACTTGTCCATTCCAGTGGATGAGGAGGACCCTT
  CAGAAGACATATTTACAGTTTTGACAGCTGAAGAAAAGCAGCGATTGGAAGAGCTCCAGCTGTTCAAAAA
  TAAGGAAACCAGTGTTGCCATTGAGGTTATCGAGGACACCAAAGAGAAAAGAACCATCATCCATCAGGCT
  ATCAAATCTCTGTTTCCAGGATTAGAGACAAAAACAGAGGATAGGGAGGGGAAGAAATACATTGTAGCCT
  ACCACGCAGCTGGGAAAAAGGCTTTGGCAAATCCAAGAAAACATTCTTGGCCAAAATCTAGGGGAAGTTA
  CTGCCACTTCGTACTATATAAGGAAAACAAAGACACCATGGATGCTATTAATGTACTCTCCAAATACTTA
  AGAGTCAAGCCAAATATATTCTCCTACATGGGAACCAAAGATAAAAGGGCTATAACAGTTCAAGAAATTG
  CTGTTCTCAAAATAACTGCACAAAGACTTGCCCACCTGAATAAGTGCTTGATGAACTTTAAGCTAGGGAA
  TTTCAGCTATCAAAAAAACCCACTGAAATTGGGAGAGCTTCAAGGAAACCACTTCACTGTTGTTCTCAGA
  AATATAACAGGAACTGATGACCAAGTACAGCAAGCTATGAACTCTCTCAAGGAGATTGGATTTATTAACT
  ACTATGGAATGCAAAGATTTGGAACCACAGCTGTCCCTACGTATCAGGTTGGAAGAGCTATACTACAAAA
  TTCCTGGACAGAAGTCATGGATTTAATATTGAAACCCCGCTCTGGAGCTGAAAAGGGCTACTTGGTTAAA
  TGCAGAGAAGAATGGGCAAAGACCAAAGACCCAACTGCTGCCCTCAGAAAACTACCTGTCAAAAGGTGTG
  TGGAAGGGCAGCTGCTTCGAGGACTTTCAAAATATGGAATGAAGAATATAGTCTCTGCATTTGGCATAAT
  ACCCAGAAATAATCGCTTAATGTATATTCATAGCTACCAAAGCTATGTGTGGAATAACATGGTAAGCAAG
  AGGATAGAAGACTATGGACTAAAACCTGTTCCAGGGGACCTCGTTCTCAAAGGAGCCACAGCCACCTATA
  TTGAGGAAGATGATGTTAATAATTACTCTATCCATGATGTGGTAATGCCCTTGCCTGGTTTCGATGTTAT
  CTACCCAAAGCATAAAATTCAAGAAGCCTACAGGGAAATGCTCACAGCTGACAATCTTGATATTGACAAC
  ATGAGACACAAAATTCGAGATTATTCCTTGTCAGGGGCCTACCGAAAGATCATTATTCGTCCTCAGAATG
  TTAGCTGGGAAGTCGTTGCATATGATGATCCCAAAATTCCACTTTTCAACACAGATGTGGACAACCTAGA
  AGGGAAGACACCACCAGTTTTTGCTTCTGAAGGCAAATACAGGGCTCTGAAAATGGATTTTTCTCTACCC
  CCTTCTACTTACGCCACCATGGCCATTCGAGAAGTGCTAAAAATGGATACCAGTATCAAGAACCAGACGC
  AGCTGAATACAACCTGGCTTCGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203902 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEMTEMTGVSLKRGALVVEDNDSGVPVEETKKQKLSECSLTKGQDGLQNDFLSISEDVPRPPDTVSTGKG
  GKNSEAQLEDEEEEEEDGLSEECEEEESESFADMMKHGLTEADVGITKFVSSHQGFSGILKERYSDFVVH
  EIGKDGRISHLNDLSIPVDEEDPSEDIFTVLTAEEKQRLEELQLFKNKETSVAIEVIEDTKEKRTIIHQA
  IKSLFPGLETKTEDREGKKYIVAYHAAGKKALANPRKHSWPKSRGSYCHFVLYKENKDTMDAINVLSKYL
  RVKPNIFSYMGTKDKRAITVQEIAVLKITAQRLAHLNKCLMNFKLGNFSYQKNPLKLGELQGNHFTVVLR
  NITGTDDQVQQAMNSLKEIGFINYYGMQRFGTTAVPTYQVGRAILQNSWTEVMDLILKPRSGAEKGYLVK
  CREEWAKTKDPTAALRKLPVKRCVEGQLLRGLSKYGMKNIVSAFGIIPRNNRLMYIHSYQSYVWNNMVSK
  RIEDYGLKPVPGDLVLKGATATYIEEDDVNNYSIHDVVMPLPGFDVIYPKHKIQEAYREMLTADNLDIDN
  MRHKIRDYSLSGAYRKIIIRPQNVSWEVVAYDDPKIPLFNTDVDNLEGKTPPVFASEGKYRALKMDFSLP
  PSTYATMAIREVLKMDTSIKNQTQLNTTWLR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019042
• ORF Size: 1983 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019042.5
• RefSeq Size: 3484 bp
• RefSeq ORF: 1986 bp
• Locus ID: 54517
• UniProt ID: Q96PZ0
• Cytogenetics: 7q22.3
• MW: 75 kDa
• Gene Summary: Pseudouridylate synthase that catalyzes pseudouridylation of RNAs (PubMed:28073919, PubMed:29628141). Acts as a regulator of protein synthesis in embryonic stem cells by mediating pseudouridylation of RNA fragments derived from tRNAs (tRFs): pseudouridylated tRFs inhibit translation by targeting the translation initiation complex (PubMed:29628141). Also catalyzes pseudouridylation of mRNAs: mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3' (PubMed:28073919). In addition to mRNAs and tRNAs, binds other types of RNAs, such as snRNAs, Y RNAs and vault RNAs, suggesting that it can catalyze pseudouridylation of many RNA types (PubMed:29628141).[UniProtKB/Swiss-Prot Function]