PUS7 (NM_019042) Human Tagged ORF Clone

CAT#: RC203902

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PUS7 (Myc-DDK-tagged)-Human pseudouridylate synthase 7 homolog (S. cerevisiae) (PUS7)



  "NM_019042" in other vectors (5)

Reconstitution Protocol

USD 615.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PUS7
Synonyms IDDABS
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC203902 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGATGACAGAAATGACTGGTGTGTCGCTGAAACGTGGGGCACTGGTTGTCGAAGATAATGACAGTG
GAGTCCCAGTTGAAGAGACAAAAAAACAGAAGCTGTCGGAATGCAGCCTAACCAAAGGTCAAGATGGGCT
ACAGAATGACTTTCTGTCCATCAGTGAAGACGTGCCTCGGCCTCCTGACACTGTCAGTACTGGGAAAGGT
GGAAAGAATTCTGAGGCTCAGTTGGAAGATGAGGAAGAAGAGGAGGAAGATGGACTTTCAGAGGAGTGCG
AGGAGGAGGAATCAGAGAGTTTTGCAGACATGATGAAGCATGGACTCACTGAGGCTGACGTAGGCATCAC
CAAGTTTGTGAGTTCTCATCAAGGGTTCTCGGGAATCTTAAAAGAAAGATACTCCGACTTCGTTGTTCAT
GAAATAGGAAAAGATGGACGGATCAGCCATTTGAATGACTTGTCCATTCCAGTGGATGAGGAGGACCCTT
CAGAAGACATATTTACAGTTTTGACAGCTGAAGAAAAGCAGCGATTGGAAGAGCTCCAGCTGTTCAAAAA
TAAGGAAACCAGTGTTGCCATTGAGGTTATCGAGGACACCAAAGAGAAAAGAACCATCATCCATCAGGCT
ATCAAATCTCTGTTTCCAGGATTAGAGACAAAAACAGAGGATAGGGAGGGGAAGAAATACATTGTAGCCT
ACCACGCAGCTGGGAAAAAGGCTTTGGCAAATCCAAGAAAACATTCTTGGCCAAAATCTAGGGGAAGTTA
CTGCCACTTCGTACTATATAAGGAAAACAAAGACACCATGGATGCTATTAATGTACTCTCCAAATACTTA
AGAGTCAAGCCAAATATATTCTCCTACATGGGAACCAAAGATAAAAGGGCTATAACAGTTCAAGAAATTG
CTGTTCTCAAAATAACTGCACAAAGACTTGCCCACCTGAATAAGTGCTTGATGAACTTTAAGCTAGGGAA
TTTCAGCTATCAAAAAAACCCACTGAAATTGGGAGAGCTTCAAGGAAACCACTTCACTGTTGTTCTCAGA
AATATAACAGGAACTGATGACCAAGTACAGCAAGCTATGAACTCTCTCAAGGAGATTGGATTTATTAACT
ACTATGGAATGCAAAGATTTGGAACCACAGCTGTCCCTACGTATCAGGTTGGAAGAGCTATACTACAAAA
TTCCTGGACAGAAGTCATGGATTTAATATTGAAACCCCGCTCTGGAGCTGAAAAGGGCTACTTGGTTAAA
TGCAGAGAAGAATGGGCAAAGACCAAAGACCCAACTGCTGCCCTCAGAAAACTACCTGTCAAAAGGTGTG
TGGAAGGGCAGCTGCTTCGAGGACTTTCAAAATATGGAATGAAGAATATAGTCTCTGCATTTGGCATAAT
ACCCAGAAATAATCGCTTAATGTATATTCATAGCTACCAAAGCTATGTGTGGAATAACATGGTAAGCAAG
AGGATAGAAGACTATGGACTAAAACCTGTTCCAGGGGACCTCGTTCTCAAAGGAGCCACAGCCACCTATA
TTGAGGAAGATGATGTTAATAATTACTCTATCCATGATGTGGTAATGCCCTTGCCTGGTTTCGATGTTAT
CTACCCAAAGCATAAAATTCAAGAAGCCTACAGGGAAATGCTCACAGCTGACAATCTTGATATTGACAAC
ATGAGACACAAAATTCGAGATTATTCCTTGTCAGGGGCCTACCGAAAGATCATTATTCGTCCTCAGAATG
TTAGCTGGGAAGTCGTTGCATATGATGATCCCAAAATTCCACTTTTCAACACAGATGTGGACAACCTAGA
AGGGAAGACACCACCAGTTTTTGCTTCTGAAGGCAAATACAGGGCTCTGAAAATGGATTTTTCTCTACCC
CCTTCTACTTACGCCACCATGGCCATTCGAGAAGTGCTAAAAATGGATACCAGTATCAAGAACCAGACGC
AGCTGAATACAACCTGGCTTCGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC203902 protein sequence
Red=Cloning site Green=Tags(s)

MEMTEMTGVSLKRGALVVEDNDSGVPVEETKKQKLSECSLTKGQDGLQNDFLSISEDVPRPPDTVSTGKG
GKNSEAQLEDEEEEEEDGLSEECEEEESESFADMMKHGLTEADVGITKFVSSHQGFSGILKERYSDFVVH
EIGKDGRISHLNDLSIPVDEEDPSEDIFTVLTAEEKQRLEELQLFKNKETSVAIEVIEDTKEKRTIIHQA
IKSLFPGLETKTEDREGKKYIVAYHAAGKKALANPRKHSWPKSRGSYCHFVLYKENKDTMDAINVLSKYL
RVKPNIFSYMGTKDKRAITVQEIAVLKITAQRLAHLNKCLMNFKLGNFSYQKNPLKLGELQGNHFTVVLR
NITGTDDQVQQAMNSLKEIGFINYYGMQRFGTTAVPTYQVGRAILQNSWTEVMDLILKPRSGAEKGYLVK
CREEWAKTKDPTAALRKLPVKRCVEGQLLRGLSKYGMKNIVSAFGIIPRNNRLMYIHSYQSYVWNNMVSK
RIEDYGLKPVPGDLVLKGATATYIEEDDVNNYSIHDVVMPLPGFDVIYPKHKIQEAYREMLTADNLDIDN
MRHKIRDYSLSGAYRKIIIRPQNVSWEVVAYDDPKIPLFNTDVDNLEGKTPPVFASEGKYRALKMDFSLP
PSTYATMAIREVLKMDTSIKNQTQLNTTWLR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_019042
ORF Size 1983 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_019042.5
RefSeq Size 3484 bp
RefSeq ORF 1986 bp
Locus ID 54517
UniProt ID Q96PZ0
Cytogenetics 7q22.3
MW 75 kDa
Gene Summary Pseudouridylate synthase that catalyzes pseudouridylation of RNAs (PubMed:28073919, PubMed:29628141). Acts as a regulator of protein synthesis in embryonic stem cells by mediating pseudouridylation of RNA fragments derived from tRNAs (tRFs): pseudouridylated tRFs inhibit translation by targeting the translation initiation complex (PubMed:29628141). Also catalyzes pseudouridylation of mRNAs: mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3' (PubMed:28073919). In addition to mRNAs and tRNAs, binds other types of RNAs, such as snRNAs, Y RNAs and vault RNAs, suggesting that it can catalyze pseudouridylation of many RNA types (PubMed:29628141).[UniProtKB/Swiss-Prot Function]

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