TMEM187 (NM_003492) Human Tagged ORF Clone

CAT#: RC203898

TMEM187 (Myc-DDK-tagged)-Human transmembrane protein 187 (TMEM187)

Product Images

Circular map for RC203898

• 

  Western blot validation of overexpression lysate (Cat# LY418645) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203898 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TMEM187
• Synonyms: CXorf12; DXS9878E; ITBA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203898 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCCAGAGTGGGGGCAGGCCTTCGTGCACGTGGCCGTGGCCGGTGGCCTCTGTGCCGTGGCTGTGT
  TCACGGGCATTTTCGACAGTGTTTCCGTGCAAGTGGGCTATGAGCACTACGCCGAGGCGCCCGTGGCCGG
  CCTCCCTGCCTTCCTGGCCATGCCGTTCAACTCACTCGTGAACATGGCCTACACGCTGCTGGGGCTGTCG
  TGGCTGCACAGGGGCGGCGCGATGGGGCTGGGTCCCCGCTACCTGAAGGACGTGTTCGCAGCCATGGCCC
  TGCTCTATGGCCCCGTGCAGTGGCTGCGCCTGTGGACGCAGTGGCGCCGTGCCGCGGTGCTGGACCAGTG
  GCTCACACTGCCCATCTTTGCATGGCCCGTGGCCTGGTGCCTCTACCTAGACCGCGGCTGGCGGCCCTGG
  CTGTTCCTCTCTCTTGAGTGCGTCTCCCTGGCCAGTTATGGCCTCGCTCTGCTGCATCCCCAGGGCTTCG
  AGGTCGCACTGGGTGCTCACGTGGTGGCCGCTGTGGGGCAGGCGCTGCGCACCCACAGGCACTATGGCAG
  CACCACCTCGGCTACCTACTTAGCTTTGGGGGTGCTCTCTTGCCTGGGCTTTGTGGTCCTCAAGCTGTGT
  GACCATCAGCTCGCACGGTGGCGTCTCTTCCAGTGCCTCACAGGCCACTTCTGGTCCAAGGTCTGTGACG
  TGCTCCAGTTCCACTTTGCGTTTTTGTTTCTGACGCATTTCAACACTCACCCAAGATTCCATCCCTCTGG
  CGGGAAGACGCGT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203898 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNPEWGQAFVHVAVAGGLCAVAVFTGIFDSVSVQVGYEHYAEAPVAGLPAFLAMPFNSLVNMAYTLLGLS
  WLHRGGAMGLGPRYLKDVFAAMALLYGPVQWLRLWTQWRRAAVLDQWLTLPIFAWPVAWCLYLDRGWRPW
  LFLSLECVSLASYGLALLHPQGFEVALGAHVVAAVGQALRTHRHYGSTTSATYLALGVLSCLGFVVLKLC
  DHQLARWRLFQCLTGHFWSKVCDVLQFHFAFLFLTHFNTHPRFHPSGGKTR
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003492
• ORF Size: 783 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003492.3
• RefSeq Size: 1682 bp
• RefSeq ORF: 786 bp
• Locus ID: 8269
• UniProt ID: Q14656
• Cytogenetics: Xq28
• Protein Families: Transmembrane
• MW: 29.1 kDa
• Gene Summary: This gene consists of two exons and encodes a multi-pass membrane protein. An alternatively spliced transcript variant encoding the same protein has been found, but its biological validity is not determined. [provided by RefSeq, May 2010]