MOSC2 (MARC2) (NM_017898) Human Tagged ORF Clone

CAT#: RC203877

MOSC2 (Myc-DDK-tagged)-Human MOCO sulphurase C-terminal domain containing 2 (MOSC2)

Product Images

Circular map for RC203877

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  Western blot validation of overexpression lysate (Cat# LY413480) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203877 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MARC2 protein (Cat# TP303877). The protein was produced from HEK293T cells transfected with MARC2 cDNA clone (Cat# RC203877) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MOSC2
• Synonyms: MARC2; MOSC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203877 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGCTTCCAGCTCCTCCGCGCTGGCCCGCCTCGGCCTCCCAGCCCGGCCCTGGCCCAGGTGGCTCG
  GGGTCGCCGCGCTAGGACTGGCCGCCGTGGCCCTGGGGACTGTCGCCTGGCGCCGCGCATGGCCCAGGCG
  GCGCCGGCGGCTGCAGCAGGTGGGCACCGTGGCGAAGCTCTGGATCTACCCGGTGAAATCCTGCAAAGGG
  GTGCCGGTGAGCGAGGCTGAGTGCACGGCCATGGGGCTGCGCAGCGGCAACCTGCGGGACAGGTTTTGGC
  TGGTGATTAAGGAAGATGGACACATGGTCACTGCCCGACAGGAGCCTCGCCTCGTGCTCATCTCCATCAT
  TTATGAGAATAACTGCCTGATCTTCAGGGCTCCAGACATGGACCAGCTGGTTTTGCCTAGCAAGCAGCCT
  TCCTCAAACAAACTCCACAACTGCAGGATATTTGGCCTTGACATTAAAGGCAGAGACTGTGGCAATGAGG
  CAGCTAAGTGGTTCACCAACTTCTTGAAAACTGAAGCGTATAGATTGGTTCAATTTGAGACAAACATGAA
  GGGAAGAACATCAAGAAAACTTCTCCCCACTCTTGATCAGAATTTCCAGGTGGCCTACCCAGACTACTGC
  CCGCTCCTGATCATGACAGATGCCTCCCTGGTAGATTTGAATACCAGGATGGAGAAGAAAATGAAAATGG
  AGAATTTCAGGCCAAATATTGTGGTGACCGGCTGTGATGCTTTTGAGGAGGATACCTGGGATGAACTCCT
  AATTGGTAGTGTAGAAGTGAAAAAGGTAATGGCATGCCCCAGGTGTATTTTGACAACGGTGGACCCAGAC
  ACTGGAGTCATAGACAGGAAACAGCCACTGGACACCCTGAAGAGCTACCGCCTGTGTGATCCTTCTGAGA
  GGGAATTGTACAAGTTGTCTCCACTTTTTGGGATCTATTATTCAGTGGAAAAAATTGGAAGCCTGAGAGT
  TGGTGACCCTGTGTATCGGATGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203877 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGASSSSALARLGLPARPWPRWLGVAALGLAAVALGTVAWRRAWPRRRRRLQQVGTVAKLWIYPVKSCKG
  VPVSEAECTAMGLRSGNLRDRFWLVIKEDGHMVTARQEPRLVLISIIYENNCLIFRAPDMDQLVLPSKQP
  SSNKLHNCRIFGLDIKGRDCGNEAAKWFTNFLKTEAYRLVQFETNMKGRTSRKLLPTLDQNFQVAYPDYC
  PLLIMTDASLVDLNTRMEKKMKMENFRPNIVVTGCDAFEEDTWDELLIGSVEVKKVMACPRCILTTVDPD
  TGVIDRKQPLDTLKSYRLCDPSERELYKLSPLFGIYYSVEKIGSLRVGDPVYRMV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017898
• ORF Size: 1005 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017898.5
• RefSeq Size: 1618 bp
• RefSeq ORF: 1008 bp
• Locus ID: 54996
• UniProt ID: Q969Z3
• Cytogenetics: 1q41
• Domains: MOSC
• Protein Families: Transmembrane
• MW: 38 kDa
• Gene Summary: The protein encoded by this gene is an enzyme found in the outer mitochondrial membrane that reduces N-hydroxylated substrates. The encoded protein uses molybdenum as a cofactor and cytochrome b5 type B and NADH cytochrome b5 reductase as accessory proteins. One type of substrate used is N-hydroxylated nucleotide base analogues, which can be toxic to a cell. Other substrates include N(omega)-hydroxy-L-arginine (NOHA) and amidoxime prodrugs, which are activated by the encoded enzyme. Multiple transcript variants encoding the different isoforms have been found for this gene. [provided by RefSeq, Sep 2016]