VPS33B (NM_018668) Human Tagged ORF Clone

CAT#: RC203870

VPS33B (Myc-DDK-tagged)-Human vacuolar protein sorting 33 homolog B (yeast) (VPS33B)

Product Images

Western blot validation of overexpression lysate (Cat# LY412974) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203870 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified VPS33B protein (Cat# TP303870). The protein was produced from HEK293T cells transfected with VPS33B cDNA clone (Cat# RC203870) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VPS33B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203870 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTTTCCCCATCGGCCGGACGCCCCTGAGCTGCCTGACTTCTCCATGCTGAAGAGGCTGGCTCGAG
  ACCAGCTCATCTATCTGCTGGAGCAGCTTCCTGGAAAAAAGGATTTATTCATTGAGGCAGATCTCATGAG
  CCCTTTGGATCGAATTGCCAATGTCTCCATCCTGAAGCAACACGAAGTAGACAAGCTATACAAGGTGGAG
  AACAAGCCAGCCCTCAGCTCCAATGAACAATTGTGCTTCTTGGTCAGACCCCGCATCAAGAATATGCGAT
  ACATTGCCAGTCTTGTCAATGCTGACAAATTGGCTGGCCGAACTCGCAAATACAAAGTGATCTTCAGCCC
  TCAAAAGTTCTATGCGTGTGAGATGGTGCTTGAGGAAGAGGGAATCTATGGAGATGTGAGCTGTGATGAA
  TGGGCCTTCTCTTTGCTGCCTCTTGATGTGGATCTGCTGAGCATGGAACTACCAGAATTTTTCAGGGATT
  ACTTTCTGGAAGGAGATCAGCGTTGGATCAACACTGTAGCTCAGGCCTTACACCTTCTCAGCACTCTCTA
  TGGACCCTTTCCAAACTGCTATGGAATTGGCAGGTGCGCCAAGATGGCATATGAATTGTGGAGGAACCTG
  GAGGAGGAGGAGGATGGCGAAACCAAGGGCCGAAGGCCAGAGATTGGACATATCTTTCTCTTGGACAGAG
  ATGTGGACTTTGTGACAGCACTTTGCTCCCAAGTGGTTTATGAGGGCCTAGTAGATGACACCTTCCGCAT
  CAAGTGTGGGAGTGTCGACTTTGGCCCAGAAGTCACATCCTCTGACAAGAGCCTGAAGGTGCTACTCAAT
  GCCGAGGACAAGGTGTTTAATGAGATTCGGAACGAGCACTTCTCCAATGTCTTTGGCTTCTTGAGCCAGA
  AGGCCCGGAACTTGCAGGCCCAGTATGATCGCCGGAGAGGCATGGACATTAAGCAGATGAAGAATTTCGT
  GTCCCAGGAGCTCAAGGGCCTGAAACAGGAGCACCGCCTGCTGAGTCTCCATATTGGGGCCTGTGAATCC
  ATCATGAAGAAGAAAACCAAGCAGGATTTCCAGGAGCTAATCAAGACTGAGCATGCACTGCTAGAGGGGT
  TCAACATCCGGGAGAGCACCAGCTACATTGAGGAACACATAGACCGGCAGGTGTCGCCTATAGAAAGCCT
  GCGCCTCATGTGCCTTTTGTCCATCACTGAGAATGGTTTGATCCCCAAGGATTACCGATCTCTGAAAACA
  CAGTATCTGCAGAGCTATGGCCCTGAGCACCTGCTAACCTTCTCCAATCTGCGAAGAGCTGGGCTCCTAA
  CGGAGCAGGCCCCCGGGGACACCCTCACAGCCGTGGAGAGTAAAGTGAGCAAGCTGGTGACCGACAAGGC
  TGCAGGAAAGATTACTGATGCCTTCAGTTCTCTGGCCAAGAGGAGCAATTTTCGTGCCATCAGCAAAAAG
  CTGAATTTGATCCCACGTGTGGACGGCGAGTATGATCTGAAAGTGCCCCGAGACATGGCTTACGTCTTCA
  GTGGTGCTTATGTGCCCCTGAGCTGCCGAATCATTGAGCAGGTGCTAGAGCGGCGAAGCTGGCAGGGCCT
  TGATGAGGTGGTACGGCTGCTCAACTGCAGTGACTTTGCATTCACAGATATGACTAAGGAAGACAAGGCT
  TCCAGTGAGTCCCTGCGCCTCATCTTGGTGGTGTTCTTGGGTGGTTGTACATTCTCTGAGATCTCAGCCC
  TCCGGTTCCTGGGCAGAGAGAAAGGCTACAGGTTCATTTTCCTGACGACAGCAGTCACAAACAGCGCTCG
  CCTTATGGAGGCCATGAGTGAGGTGAAAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203870 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAFPHRPDAPELPDFSMLKRLARDQLIYLLEQLPGKKDLFIEADLMSPLDRIANVSILKQHEVDKLYKVE
  NKPALSSNEQLCFLVRPRIKNMRYIASLVNADKLAGRTRKYKVIFSPQKFYACEMVLEEEGIYGDVSCDE
  WAFSLLPLDVDLLSMELPEFFRDYFLEGDQRWINTVAQALHLLSTLYGPFPNCYGIGRCAKMAYELWRNL
  EEEEDGETKGRRPEIGHIFLLDRDVDFVTALCSQVVYEGLVDDTFRIKCGSVDFGPEVTSSDKSLKVLLN
  AEDKVFNEIRNEHFSNVFGFLSQKARNLQAQYDRRRGMDIKQMKNFVSQELKGLKQEHRLLSLHIGACES
  IMKKKTKQDFQELIKTEHALLEGFNIRESTSYIEEHIDRQVSPIESLRLMCLLSITENGLIPKDYRSLKT
  QYLQSYGPEHLLTFSNLRRAGLLTEQAPGDTLTAVESKVSKLVTDKAAGKITDAFSSLAKRSNFRAISKK
  LNLIPRVDGEYDLKVPRDMAYVFSGAYVPLSCRIIEQVLERRSWQGLDEVVRLLNCSDFAFTDMTKEDKA
  SSESLRLILVVFLGGCTFSEISALRFLGREKGYRFIFLTTAVTNSARLMEAMSEVKA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018668
• ORF Size: 1851 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018668.2, NP_061138.2
• RefSeq Size: 2795 bp
• RefSeq ORF: 1854 bp
• Locus ID: 26276
• UniProt ID: Q9H267
• Cytogenetics: 15q26.1
• Domains: Sec1
• MW: 70.6 kDa
• Gene Summary: Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec-1 domain family, and encodes the human ortholog of rat Vps33b which is homologous to the yeast class C Vps33 protein. The mammalian class C vacuolar protein sorting proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Mutations in this gene are associated with arthrogryposis-renal dysfunction-cholestasis syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]