VPS33B (NM_018668) Human Tagged ORF Clone

CAT#: RC203870

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VPS33B (Myc-DDK-tagged)-Human vacuolar protein sorting 33 homolog B (yeast) (VPS33B)



Reconstitution Protocol

USD 861.00

In Stock*

Size
    • 10 ug

Product images

Frequently bought together (4)
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol VPS33B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC203870 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTTTCCCCATCGGCCGGACGCCCCTGAGCTGCCTGACTTCTCCATGCTGAAGAGGCTGGCTCGAG
ACCAGCTCATCTATCTGCTGGAGCAGCTTCCTGGAAAAAAGGATTTATTCATTGAGGCAGATCTCATGAG
CCCTTTGGATCGAATTGCCAATGTCTCCATCCTGAAGCAACACGAAGTAGACAAGCTATACAAGGTGGAG
AACAAGCCAGCCCTCAGCTCCAATGAACAATTGTGCTTCTTGGTCAGACCCCGCATCAAGAATATGCGAT
ACATTGCCAGTCTTGTCAATGCTGACAAATTGGCTGGCCGAACTCGCAAATACAAAGTGATCTTCAGCCC
TCAAAAGTTCTATGCGTGTGAGATGGTGCTTGAGGAAGAGGGAATCTATGGAGATGTGAGCTGTGATGAA
TGGGCCTTCTCTTTGCTGCCTCTTGATGTGGATCTGCTGAGCATGGAACTACCAGAATTTTTCAGGGATT
ACTTTCTGGAAGGAGATCAGCGTTGGATCAACACTGTAGCTCAGGCCTTACACCTTCTCAGCACTCTCTA
TGGACCCTTTCCAAACTGCTATGGAATTGGCAGGTGCGCCAAGATGGCATATGAATTGTGGAGGAACCTG
GAGGAGGAGGAGGATGGCGAAACCAAGGGCCGAAGGCCAGAGATTGGACATATCTTTCTCTTGGACAGAG
ATGTGGACTTTGTGACAGCACTTTGCTCCCAAGTGGTTTATGAGGGCCTAGTAGATGACACCTTCCGCAT
CAAGTGTGGGAGTGTCGACTTTGGCCCAGAAGTCACATCCTCTGACAAGAGCCTGAAGGTGCTACTCAAT
GCCGAGGACAAGGTGTTTAATGAGATTCGGAACGAGCACTTCTCCAATGTCTTTGGCTTCTTGAGCCAGA
AGGCCCGGAACTTGCAGGCCCAGTATGATCGCCGGAGAGGCATGGACATTAAGCAGATGAAGAATTTCGT
GTCCCAGGAGCTCAAGGGCCTGAAACAGGAGCACCGCCTGCTGAGTCTCCATATTGGGGCCTGTGAATCC
ATCATGAAGAAGAAAACCAAGCAGGATTTCCAGGAGCTAATCAAGACTGAGCATGCACTGCTAGAGGGGT
TCAACATCCGGGAGAGCACCAGCTACATTGAGGAACACATAGACCGGCAGGTGTCGCCTATAGAAAGCCT
GCGCCTCATGTGCCTTTTGTCCATCACTGAGAATGGTTTGATCCCCAAGGATTACCGATCTCTGAAAACA
CAGTATCTGCAGAGCTATGGCCCTGAGCACCTGCTAACCTTCTCCAATCTGCGAAGAGCTGGGCTCCTAA
CGGAGCAGGCCCCCGGGGACACCCTCACAGCCGTGGAGAGTAAAGTGAGCAAGCTGGTGACCGACAAGGC
TGCAGGAAAGATTACTGATGCCTTCAGTTCTCTGGCCAAGAGGAGCAATTTTCGTGCCATCAGCAAAAAG
CTGAATTTGATCCCACGTGTGGACGGCGAGTATGATCTGAAAGTGCCCCGAGACATGGCTTACGTCTTCA
GTGGTGCTTATGTGCCCCTGAGCTGCCGAATCATTGAGCAGGTGCTAGAGCGGCGAAGCTGGCAGGGCCT
TGATGAGGTGGTACGGCTGCTCAACTGCAGTGACTTTGCATTCACAGATATGACTAAGGAAGACAAGGCT
TCCAGTGAGTCCCTGCGCCTCATCTTGGTGGTGTTCTTGGGTGGTTGTACATTCTCTGAGATCTCAGCCC
TCCGGTTCCTGGGCAGAGAGAAAGGCTACAGGTTCATTTTCCTGACGACAGCAGTCACAAACAGCGCTCG
CCTTATGGAGGCCATGAGTGAGGTGAAAGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC203870 protein sequence
Red=Cloning site Green=Tags(s)

MAFPHRPDAPELPDFSMLKRLARDQLIYLLEQLPGKKDLFIEADLMSPLDRIANVSILKQHEVDKLYKVE
NKPALSSNEQLCFLVRPRIKNMRYIASLVNADKLAGRTRKYKVIFSPQKFYACEMVLEEEGIYGDVSCDE
WAFSLLPLDVDLLSMELPEFFRDYFLEGDQRWINTVAQALHLLSTLYGPFPNCYGIGRCAKMAYELWRNL
EEEEDGETKGRRPEIGHIFLLDRDVDFVTALCSQVVYEGLVDDTFRIKCGSVDFGPEVTSSDKSLKVLLN
AEDKVFNEIRNEHFSNVFGFLSQKARNLQAQYDRRRGMDIKQMKNFVSQELKGLKQEHRLLSLHIGACES
IMKKKTKQDFQELIKTEHALLEGFNIRESTSYIEEHIDRQVSPIESLRLMCLLSITENGLIPKDYRSLKT
QYLQSYGPEHLLTFSNLRRAGLLTEQAPGDTLTAVESKVSKLVTDKAAGKITDAFSSLAKRSNFRAISKK
LNLIPRVDGEYDLKVPRDMAYVFSGAYVPLSCRIIEQVLERRSWQGLDEVVRLLNCSDFAFTDMTKEDKA
SSESLRLILVVFLGGCTFSEISALRFLGREKGYRFIFLTTAVTNSARLMEAMSEVKA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_018668
ORF Size 1851 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_018668.2, NP_061138.2
RefSeq Size 2795 bp
RefSeq ORF 1854 bp
Locus ID 26276
UniProt ID Q9H267
Cytogenetics 15q26.1
Domains Sec1
MW 70.6 kDa
Gene Summary Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec-1 domain family, and encodes the human ortholog of rat Vps33b which is homologous to the yeast class C Vps33 protein. The mammalian class C vacuolar protein sorting proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Mutations in this gene are associated with arthrogryposis-renal dysfunction-cholestasis syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
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