CALCOCO2 (NM_005831) Human Tagged ORF Clone

CAT#: RC203843

CALCOCO2 (Myc-DDK-tagged)-Human calcium binding and coiled-coil domain 2 (CALCOCO2)

Product Images

Circular map for RC203843

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CALCOCO2 (Cat# RC203843, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CALCOCO2(Cat# TA501971). Positive lysates LY417045 (100ug) and LC417045 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY417045) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203843 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CALCOCO2 protein (Cat# TP303843). The protein was produced from HEK293T cells transfected with CALCOCO2 cDNA clone (Cat# RC203843) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CALCOCO2
• Synonyms: NDP52
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203843 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGACCATCAAAGATCCCCCCACATCAGCTGTCTTGCTGGATCACTGTCATTTCTCTCAGGTCA
  TCTTTAACAGTGTGGAGAAGTTCTACATCCCTGGAGGGGACGTCACATGTCATTATACCTTCACCCAGCA
  TTTCATCCCTCGTCGAAAGGATTGGATTGGCATCTTTAGAGTGGGGTGGAAGACAACCCGTGAGTATTAC
  ACCTTCATGTGGGTTACTTTGCCCATTGACCTAAACAACAAATCAGCTAAACAGCAGGAAGTCCAATTCA
  AAGCTTACTACCTGCCCAAGGATGATGAGTATTACCAGTTCTGCTATGTGGATGAGGATGGTGTGGTCCG
  GGGAGCAAGTATTCCTTTCCAATTCCGTCCAGAAAATGAGGAAGACATCCTGGTTGTTACCACTCAGGGA
  GAGGTGGAAGAGATTGAGCAGCACAACAAGGAGCTTTGCAAAGAAAACCAGGAGCTGAAGGACAGCTGTA
  TCAGCCTCCAGAAGCAGAACTCAGACATGCAGGCTGAGCTCCAAAAGAAGCAGGAGGAGCTAGAAACCCT
  ACAGAGCATCAATAAGAAGTTGGAACTGAAAGTGAAAGAACAGAAGGACTATTGGGAGACAGAGCTGCTT
  CAACTGAAAGAACAAAACCAGAAGATGTCCTCAGAAAATGAGAAGATGGGAATCAGAGTGGATCAGCTTC
  AGGCCCAGCTGTCAACTCAAGAGAAAGAAATGGAGAAGCTTGTTCAGGGAGATCAAGATAAGACAGAGCA
  GTTAGAGCAGCTGAAAAAGGAAAATGACCACCTCTTTCTCAGTTTAACTGAACAGAGGAAGGACCAGAAG
  AAGCTCGAGCAGACAGTGGAGCAAATGAAGCAGAATGAAACTACTGCAATGAAGAAACAACAGGAATTAA
  TGGATGAAAACTTTGACCTGTCAAAAAGACTGAGTGAGAACGAAATTATATGTAATGCTCTGCAGAGACA
  GAAAGAGAGATTGGAAGGAGAAAATGATCTTTTGAAGAGGGAGAACAGCAGATTGCTCAGTTACATGGGT
  CTGGATTTTAATTCTTTGCCGTATCAAGTACCTACTTCAGATGAAGGAGGCGCAAGACAAAATCCAGGAC
  TTGCCTATGGAAACCCATATTCTGGTATCCAAGAAAGTTCTTCCCCCAGCCCGCTCTCCATCAAGAAATG
  CCCTATCTGCAAAGCAGATGATATTTGTGATCACACCTTGGAGCAACAGCAGATGCAGCCCCTTTGTTTC
  AATTGTCCAATTTGTGACAAGATCTTCCCAGCTACAGAGAAGCAGATCTTTGAAGACCACGTGTTCTGCC
  ACTCTCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203843 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEETIKDPPTSAVLLDHCHFSQVIFNSVEKFYIPGGDVTCHYTFTQHFIPRRKDWIGIFRVGWKTTREYY
  TFMWVTLPIDLNNKSAKQQEVQFKAYYLPKDDEYYQFCYVDEDGVVRGASIPFQFRPENEEDILVVTTQG
  EVEEIEQHNKELCKENQELKDSCISLQKQNSDMQAELQKKQEELETLQSINKKLELKVKEQKDYWETELL
  QLKEQNQKMSSENEKMGIRVDQLQAQLSTQEKEMEKLVQGDQDKTEQLEQLKKENDHLFLSLTEQRKDQK
  KLEQTVEQMKQNETTAMKKQQELMDENFDLSKRLSENEIICNALQRQKERLEGENDLLKRENSRLLSYMG
  LDFNSLPYQVPTSDEGGARQNPGLAYGNPYSGIQESSSPSPLSIKKCPICKADDICDHTLEQQQMQPLCF
  NCPICDKIFPATEKQIFEDHVFCHSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005831
• ORF Size: 1338 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005831.5
• RefSeq Size: 3682 bp
• RefSeq ORF: 1341 bp
• Locus ID: 10241
• UniProt ID: Q13137
• Cytogenetics: 17q21.32
• MW: 52.3 kDa
• Gene Summary: This gene encodes a coiled-coil domain-containing protein. The encoded protein functions as a receptor for ubiquitin-coated bacteria and plays an important role in innate immunity by mediating macroautophagy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]