EIF3D (NM_003753) Human Tagged ORF Clone

CAT#: RC203830

EIF3D (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 3, subunit D (EIF3D)

Product Images

Circular map for RC203830

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY EIF3D (Cat# RC203830, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF3D (Cat# TA808199)(1:2000). Positive lysates LY401237 (100ug) and LC401237 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY401237) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203830 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified EIF3D protein (Cat# TP303830). The protein was produced from HEK293T cells transfected with EIF3D cDNA clone (Cat# RC203830) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EIF3D
• Synonyms: eIF3-p66; eIF3-zeta; EIF3S7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203830 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAAGTTCATGACACCCGTGATCCAGGACAACCCCTCAGGCTGGGGTCCCTGTGCGGTTCCCGAGC
  AGTTTCGGGATATGCCCTACCAGCCGTTCAGCAAAGGAGATCGGCTAGGAAAGGTTGCAGACTGGACAGG
  AGCCACATACCAAGATAAGAGGTACACAAATAAGTACTCCTCTCAGTTTGGTGGTGGAAGTCAATATGCT
  TATTTCCATGAGGAGGATGAAAGTAGCTTCCAGCTGGTGGATACAGCGCGCACACAGAAGACGGCCTACC
  AGCGGAATCGAATGAGATTTGCCCAGAGGAACCTCCGCAGAGACAAAGATCGTCGGAACATGTTGCAGTT
  CAACCTGCAGATCCTGCCTAAGAGTGCCAAACAGAAAGAGAGAGAACGCATTCGACTGCAGAAAAAGTTC
  CAGAAACAATTTGGGGTTAGGCAGAAATGGGATCAGAAATCACAGAAACCCCGAGACTCTTCAGTTGAAG
  TTCGTAGTGATTGGGAAGTGAAAGAGGAAATGGATTTTCCTCAGTTGATGAAGATGCGCTACTTGGAAGT
  ATCAGAGCCACAGGACATTGAGTGTTGTGGGGCCCTAGAATACTACGACAAAGCCTTTGACCGCATCACC
  ACGAGGAGTGAGAAGCCACTGCGGAGCATCAAGCGCATCTTCCACACTGTCACCACCACAGACGACCCTG
  TCATCCGCAAGCTGGCAAAAACTCAGGGGAATGTGTTTGCCACTGATGCCATCCTGGCCACGCTGATGAG
  CTGTACCCGCTCAGTGTATTCCTGGGATATTGTCGTCCAGAGAGTTGGGTCCAAACTCTTCTTTGACAAG
  AGAGACAACTCTGACTTTGACCTCCTGACAGTGAGTGAGACTGCCAATGAGCCCCCTCAAGATGAAGGTA
  ATTCCTTCAATTCACCCCGCAACCTGGCCATGGAGGCAACCTACATCAACCACAATTTCTCCCAGCAGTG
  CTTGAGAATGGGGAAGGAAAGATACAACTTCCCCAACCCAAACCCGTTTGTGGAGGACGACATGGATAAG
  AATGAAATCGCCTCTGTTGCGTACCGTTACCGCAGGTGGAAGCTTGGAGATGATATTGACCTTATTGTCC
  GTTGTGAGCACGATGGCGTCATGACTGGAGCCAACGGGGAAGTGTCCTTCATCAACATCAAGACACTCAA
  TGAGTGGGATTCCAGGCACTGTAATGGCGTTGACTGGCGTCAGAAGCTGGACTCTCAGCGAGGGGCTGTC
  ATTGCCACGGAGCTGAAGAACAACAGCTACAAGTTGGCCCGGTGGACCTGCTGTGCTTTGCTGGCTGGAT
  CTGAGTACCTCAAGCTTGGTTATGTGTCTCGGTACCACGTGAAAGACTCCTCACGCCACGTCATCCTAGG
  CACCCAGCAGTTCAAGCCTAATGAGTTTGCCAGCCAGATCAACCTGAGCGTGGAGAATGCCTGGGGCATT
  TTACGCTGCGTCATTGACATCTGCATGAAGCTGGAGGAGGGCAAATACCTCATCCTCAAGGACCCCAACA
  AGCAGGTCATCCGTGTCTACAGCCTCCCTGATGGCACCTTCAGCTCTGATGAAGATGAGGAGGAAGAGGA
  GGAGGAAGAAGAGGAAGAAGAAGAGGAAGAAACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203830 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAKFMTPVIQDNPSGWGPCAVPEQFRDMPYQPFSKGDRLGKVADWTGATYQDKRYTNKYSSQFGGGSQYA
  YFHEEDESSFQLVDTARTQKTAYQRNRMRFAQRNLRRDKDRRNMLQFNLQILPKSAKQKERERIRLQKKF
  QKQFGVRQKWDQKSQKPRDSSVEVRSDWEVKEEMDFPQLMKMRYLEVSEPQDIECCGALEYYDKAFDRIT
  TRSEKPLRSIKRIFHTVTTTDDPVIRKLAKTQGNVFATDAILATLMSCTRSVYSWDIVVQRVGSKLFFDK
  RDNSDFDLLTVSETANEPPQDEGNSFNSPRNLAMEATYINHNFSQQCLRMGKERYNFPNPNPFVEDDMDK
  NEIASVAYRYRRWKLGDDIDLIVRCEHDGVMTGANGEVSFINIKTLNEWDSRHCNGVDWRQKLDSQRGAV
  IATELKNNSYKLARWTCCALLAGSEYLKLGYVSRYHVKDSSRHVILGTQQFKPNEFASQINLSVENAWGI
  LRCVIDICMKLEEGKYLILKDPNKQVIRVYSLPDGTFSSDEDEEEEEEEEEEEEEEET
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003753
• ORF Size: 1644 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_003753.4
• RefSeq Size: 1949 bp
• RefSeq ORF: 1647 bp
• Locus ID: 8664
• UniProt ID: O15371
• Cytogenetics: 22q12.3
• Domains: eIF-3_zeta
• MW: 64 kDa
• Gene Summary: Eukaryotic translation initiation factor-3 (eIF3), the largest of the eIFs, is a multiprotein complex composed of at least ten nonidentical subunits. The complex binds to the 40S ribosome and helps maintain the 40S and 60S ribosomal subunits in a dissociated state. It is also thought to play a role in the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA, and by promoting mRNA binding. The protein encoded by this gene is the major RNA binding subunit of the eIF3 complex. [provided by RefSeq, Jul 2008]