EIF3D (NM_003753) Human Tagged ORF Clone

CAT#: RC203830

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EIF3D (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 3, subunit D (EIF3D)



  "NM_003753" in other vectors (5)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


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Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol EIF3D
Synonyms eIF3-p66; eIF3-zeta; EIF3S7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC203830 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAAAGTTCATGACACCCGTGATCCAGGACAACCCCTCAGGCTGGGGTCCCTGTGCGGTTCCCGAGC
AGTTTCGGGATATGCCCTACCAGCCGTTCAGCAAAGGAGATCGGCTAGGAAAGGTTGCAGACTGGACAGG
AGCCACATACCAAGATAAGAGGTACACAAATAAGTACTCCTCTCAGTTTGGTGGTGGAAGTCAATATGCT
TATTTCCATGAGGAGGATGAAAGTAGCTTCCAGCTGGTGGATACAGCGCGCACACAGAAGACGGCCTACC
AGCGGAATCGAATGAGATTTGCCCAGAGGAACCTCCGCAGAGACAAAGATCGTCGGAACATGTTGCAGTT
CAACCTGCAGATCCTGCCTAAGAGTGCCAAACAGAAAGAGAGAGAACGCATTCGACTGCAGAAAAAGTTC
CAGAAACAATTTGGGGTTAGGCAGAAATGGGATCAGAAATCACAGAAACCCCGAGACTCTTCAGTTGAAG
TTCGTAGTGATTGGGAAGTGAAAGAGGAAATGGATTTTCCTCAGTTGATGAAGATGCGCTACTTGGAAGT
ATCAGAGCCACAGGACATTGAGTGTTGTGGGGCCCTAGAATACTACGACAAAGCCTTTGACCGCATCACC
ACGAGGAGTGAGAAGCCACTGCGGAGCATCAAGCGCATCTTCCACACTGTCACCACCACAGACGACCCTG
TCATCCGCAAGCTGGCAAAAACTCAGGGGAATGTGTTTGCCACTGATGCCATCCTGGCCACGCTGATGAG
CTGTACCCGCTCAGTGTATTCCTGGGATATTGTCGTCCAGAGAGTTGGGTCCAAACTCTTCTTTGACAAG
AGAGACAACTCTGACTTTGACCTCCTGACAGTGAGTGAGACTGCCAATGAGCCCCCTCAAGATGAAGGTA
ATTCCTTCAATTCACCCCGCAACCTGGCCATGGAGGCAACCTACATCAACCACAATTTCTCCCAGCAGTG
CTTGAGAATGGGGAAGGAAAGATACAACTTCCCCAACCCAAACCCGTTTGTGGAGGACGACATGGATAAG
AATGAAATCGCCTCTGTTGCGTACCGTTACCGCAGGTGGAAGCTTGGAGATGATATTGACCTTATTGTCC
GTTGTGAGCACGATGGCGTCATGACTGGAGCCAACGGGGAAGTGTCCTTCATCAACATCAAGACACTCAA
TGAGTGGGATTCCAGGCACTGTAATGGCGTTGACTGGCGTCAGAAGCTGGACTCTCAGCGAGGGGCTGTC
ATTGCCACGGAGCTGAAGAACAACAGCTACAAGTTGGCCCGGTGGACCTGCTGTGCTTTGCTGGCTGGAT
CTGAGTACCTCAAGCTTGGTTATGTGTCTCGGTACCACGTGAAAGACTCCTCACGCCACGTCATCCTAGG
CACCCAGCAGTTCAAGCCTAATGAGTTTGCCAGCCAGATCAACCTGAGCGTGGAGAATGCCTGGGGCATT
TTACGCTGCGTCATTGACATCTGCATGAAGCTGGAGGAGGGCAAATACCTCATCCTCAAGGACCCCAACA
AGCAGGTCATCCGTGTCTACAGCCTCCCTGATGGCACCTTCAGCTCTGATGAAGATGAGGAGGAAGAGGA
GGAGGAAGAAGAGGAAGAAGAAGAGGAAGAAACT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC203830 protein sequence
Red=Cloning site Green=Tags(s)

MAKFMTPVIQDNPSGWGPCAVPEQFRDMPYQPFSKGDRLGKVADWTGATYQDKRYTNKYSSQFGGGSQYA
YFHEEDESSFQLVDTARTQKTAYQRNRMRFAQRNLRRDKDRRNMLQFNLQILPKSAKQKERERIRLQKKF
QKQFGVRQKWDQKSQKPRDSSVEVRSDWEVKEEMDFPQLMKMRYLEVSEPQDIECCGALEYYDKAFDRIT
TRSEKPLRSIKRIFHTVTTTDDPVIRKLAKTQGNVFATDAILATLMSCTRSVYSWDIVVQRVGSKLFFDK
RDNSDFDLLTVSETANEPPQDEGNSFNSPRNLAMEATYINHNFSQQCLRMGKERYNFPNPNPFVEDDMDK
NEIASVAYRYRRWKLGDDIDLIVRCEHDGVMTGANGEVSFINIKTLNEWDSRHCNGVDWRQKLDSQRGAV
IATELKNNSYKLARWTCCALLAGSEYLKLGYVSRYHVKDSSRHVILGTQQFKPNEFASQINLSVENAWGI
LRCVIDICMKLEEGKYLILKDPNKQVIRVYSLPDGTFSSDEDEEEEEEEEEEEEEEET

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003753
ORF Size 1644 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003753.4
RefSeq Size 1949 bp
RefSeq ORF 1647 bp
Locus ID 8664
UniProt ID O15371
Cytogenetics 22q12.3
Domains eIF-3_zeta
MW 64 kDa
Gene Summary Eukaryotic translation initiation factor-3 (eIF3), the largest of the eIFs, is a multiprotein complex composed of at least ten nonidentical subunits. The complex binds to the 40S ribosome and helps maintain the 40S and 60S ribosomal subunits in a dissociated state. It is also thought to play a role in the formation of the 40S initiation complex by interacting with the ternary complex of eIF2/GTP/methionyl-tRNA, and by promoting mRNA binding. The protein encoded by this gene is the major RNA binding subunit of the eIF3 complex. [provided by RefSeq, Jul 2008]

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