HLA-DOA (NM_002119) Human Tagged ORF Clone

CAT#: RC203816

HLA (Myc-DDK-tagged)-Human major histocompatibility complex, class II, DO alpha (HLA-DOA)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro

AAV Particle: DDK


  "NM_002119" in other vectors (4)

Reconstitution Protocol

USD 300.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

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HLA-DOA rabbit polyclonal antibody
    • 100 ul

USD 380.00

Other products for "HLA-DOA"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol HLA-DOA
Synonyms HLA-DNA; HLA-DZA; HLADZ
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC203816 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCTCAGAGCAGGGCTGGTCCTGGGGTTCCACACCCTGATGACCCTCCTGAGCCCGCAGGAGGCAG
GGGCCACCAAGGCTGACCACATGGGCTCCTACGGACCCGCCTTCTACCAGTCTTACGGCGCCTCGGGCCA
GTTCACCCATGAATTTGATGAGGAACAGCTGTTCTCTGTGGACCTGAAGAAAAGCGAGGCCGTGTGGCGT
CTGCCTGAGTTTGGCGACTTTGCCCGCTTTGACCCGCAGGGCGGGCTGGCCGGCATCGCCGCAATCAAAG
CCCATCTGGACATCCTGGTGGAGCGCTCCAACCGCAGCAGAGCCATCAACGTGCCTCCACGGGTGACCGT
GCTCCCCAAATCTCGGGTGGAGCTGGGCCAGCCCAACATCCTCATCTGCATCGTGGACAACATCTTCCCC
CCTGTGATCAATATCACCTGGCTGCGCAACGGCCAAACTGTCACTGAGGGAGTGGCCCAGACCAGCTTCT
ATTCCCAGCCTGACCATTTGTTCCGCAAGTTCCACTACCTGCCCTTCGTGCCCTCAGCCGAGGACGTCTA
TGACTGCCAGGTGGAGCACTGGGGCCTGGATGCGCCACTCCTCAGGCATTGGGAGCTCCAGGTGCCTATT
CCACCACCAGATGCCATGGAGACCCTGGTCTGTGCCCTGGGCCTGGCCATCGGCCTGGTGGGCTTCCTCG
TGGGCACCGTCCTCATCATCATGGGCACATATGTGTCCAGTGTCCCCAGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC203816 protein sequence
Red=Cloning site Green=Tags(s)

MALRAGLVLGFHTLMTLLSPQEAGATKADHMGSYGPAFYQSYGASGQFTHEFDEEQLFSVDLKKSEAVWR
LPEFGDFARFDPQGGLAGIAAIKAHLDILVERSNRSRAINVPPRVTVLPKSRVELGQPNILICIVDNIFP
PVINITWLRNGQTVTEGVAQTSFYSQPDHLFRKFHYLPFVPSAEDVYDCQVEHWGLDAPLLRHWELQVPI
PPPDAMETLVCALGLAIGLVGFLVGTVLIIMGTYVSSVPR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002119
ORF Size 750 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002119.2
RefSeq Size 3485 bp
RefSeq ORF 753 bp
Locus ID 3111
UniProt ID P06340
Cytogenetics 6p21.32
Domains MHC_II_alpha, ig, IGc1
Protein Families Transmembrane
Protein Pathways Allograft rejection, Antigen processing and presentation, Asthma, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Systemic lupus erythematosus, Type I diabetes mellitus, Viral myocarditis
MW 27.6 kDa
Gene Summary HLA-DOA belongs to the HLA class II alpha chain paralogues. HLA-DOA forms a heterodimer with HLA-DOB. The heterodimer, HLA-DO, is found in lysosomes in B cells and regulates HLA-DM-mediated peptide loading on MHC class II molecules. In comparison with classical HLA class II molecules, this gene exhibits very little sequence variation, especially at the protein level. [provided by RefSeq, Jul 2008]

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