CCR4 NOT transcription complex subunit 3 (CNOT3) (NM_014516) Human Tagged ORF Clone

CAT#: RC203795

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CNOT3 (Myc-DDK-tagged)-Human CCR4-NOT transcription complex, subunit 3 (CNOT3)



  "NM_014516" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol CNOT3
Synonyms IDDSADF; LENG2; NOT3; NOT3H
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC203795 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGACAAGCGCAAACTCCAAGGTGAGATTGATCGCTGCCTCAAGAAGGTGTCCGAGGGCGTGGAGC
AGTTTGAAGATATTTGGCAGAAGCTCCACAATGCAGCCAACGCGAACCAGAAAGAAAAGTATGAGGCTGA
CCTAAAGAAGGAGATTAAGAAGCTACAACGGCTGAGGGACCAAATCAAGACATGGGTAGCGTCCAACGAG
ATCAAGGACAAGAGGCAGCTTATAGACAACCGCAAGCTCATTGAGACGCAAATGGAACGGTTCAAAGTTG
TGGAACGAGAGACCAAAACCAAAGCTTACAGCAAAGAGGGCCTGGGCCTGGCCCAGAAGGTAGATCCTGC
CCAGAAGGAGAAGGAAGAGGTTGGCCAGTGGCTCACGAATACCATCGACACGCTCAACATGCAGGTGGAC
CAGTTTGAGAGTGAAGTGGAGTCACTGTCAGTGCAGACACGCAAGAAGAAGGGCGACAAGGATAAGCAGG
ACCGGATTGAGGGCTTGAAGCGGCACATCGAGAAGCACCGCTACCACGTGCGCATGCTAGAGACCATCCT
GCGCATGCTGGACAATGACTCCATCCTCGTTGACGCCATCCGCAAGATCAAGGACGACGTTGAGTACTAT
GTTGACTCATCCCAGGACCCCGACTTCGAGGAGAACGAGTTTCTCTACGATGACCTGGACCTCGAGGACA
TTCCACAGGCGCTGGTCGCCACCTCCCCCCCCAGCCACAGCCACATGGAGGATGAGATCTTCAACCAGTC
CAGCAGCACGCCCACCTCAACCACCTCCAGCTCTCCCATCCCGCCCAGCCCAGCCAACTGTACCACGGAA
AACTCTGAAGATGATAAGAAGAGGGGACGTTCCACAGACAGTGAAGTCAGCCAGTCTCCAGCCAAAAACG
GCTCCAAGCCTGTCCACAGCAACCAGCACCCTCAGTCCCCAGCTGTGCCGCCCACCTACCCCTCCGGCCC
CCCGCCTGCTGCCTCTGCCTTGAGCACCACTCCTGGCAACAATGGGGTCCCCGCCCCCGCAGCACCCCCA
AGTGCCCTGGGCCCCAAGGCCAGTCCAGCTCCCAGCCACAACTCGGGCACCCCTGCTCCCTATGCCCAGG
CGGTGGCCCCACCAGCTCCCAGTGGGCCCAGCACGACCCAGCCCCGGCCCCCCAGCGTCCAGCCTAGCGG
AGGCGGAGGCGGCGGCAGCGGAGGCGGAGGGAGCAGCAGCAGTAGTAACAGCAGTGCCGGTGGAGGGGCT
GGCAAGCAGAATGGCGCCACCAGTTACAGCTCAGTTGTGGCAGACAGCCCGGCAGAGGTGGCTTTGAGCA
GCAGTGGGGGCAACAATGCCAGCAGCCAGGCCTTGGGCCCCCCTTCCGGCCCCCACAACCCACCTCCCAG
CACCTCGAAGGAACCCAGTGCGGCAGCCCCAACGGGGGCTGGGGGCGTGGCCCCAGGCTCAGGGAACAAC
TCAGGGGGACCCAGCCTCCTGGTGCCACTGCCTGTGAATCCTCCCAGCTCCCCAACGCCCAGCTTCAGTG
ATGCCAAGGCAGCCGGTGCCCTGCTCAATGGGCCTCCACAGTTCAGCACCGCCCCAGAAATCAAGGCCCC
TGAGCCTCTGAGCTCCTTGAAGTCCATGGCGGAACGGGCAGCCATCAGCTCTGGCATTGAGGACCCTGTG
CCAACGCTGCACCTGACCGAGCGAGACATCATCCTGAGCAGTACATCAGCACCTCCGGCCTCAGCCCAGC
CGCCCCTGCAGCTGTCAGAGGTGAACATACCGCTGTCGCTGGGTGTCTGTCCACTGGGCCCTGTGCCCCT
CACCAAGGAGCAGCTCTATCAGCAGGCCATGGAAGAGGCCGCCTGGCACCACATGCCTCACCCCTCTGAC
TCTGAGCGTATTCGGCAGTACCTCCCCCGGAACCCCTGTCCGACGCCCCCCTACCACCACCAGATGCCAC
CCCCACACTCGGACACTGTGGAATTCTACCAGCGCCTGTCGACCGAGACACTCTTCTTCATCTTCTACTA
TCTGGAGGGCACTAAGGCACAGTATCTGGCAGCCAAGGCCCTAAAGAAGCAGTCATGGCGATTCCACACC
AAGTACATGATGTGGTTCCAGAGGCACGAGGAGCCCAAGACCATCACTGACGAGTTTGAGCAGGGCACCT
ACATCTACTTTGACTACGAGAAGTGGGGCCAGCGGAAGAAGGAAGGCTTCACCTTTGAGTACCGCTACCT
GGAGGACCGGGACCTCCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC203795 protein sequence
Red=Cloning site Green=Tags(s)

MADKRKLQGEIDRCLKKVSEGVEQFEDIWQKLHNAANANQKEKYEADLKKEIKKLQRLRDQIKTWVASNE
IKDKRQLIDNRKLIETQMERFKVVERETKTKAYSKEGLGLAQKVDPAQKEKEEVGQWLTNTIDTLNMQVD
QFESEVESLSVQTRKKKGDKDKQDRIEGLKRHIEKHRYHVRMLETILRMLDNDSILVDAIRKIKDDVEYY
VDSSQDPDFEENEFLYDDLDLEDIPQALVATSPPSHSHMEDEIFNQSSSTPTSTTSSSPIPPSPANCTTE
NSEDDKKRGRSTDSEVSQSPAKNGSKPVHSNQHPQSPAVPPTYPSGPPPAASALSTTPGNNGVPAPAAPP
SALGPKASPAPSHNSGTPAPYAQAVAPPAPSGPSTTQPRPPSVQPSGGGGGGSGGGGSSSSSNSSAGGGA
GKQNGATSYSSVVADSPAEVALSSSGGNNASSQALGPPSGPHNPPPSTSKEPSAAAPTGAGGVAPGSGNN
SGGPSLLVPLPVNPPSSPTPSFSDAKAAGALLNGPPQFSTAPEIKAPEPLSSLKSMAERAAISSGIEDPV
PTLHLTERDIILSSTSAPPASAQPPLQLSEVNIPLSLGVCPLGPVPLTKEQLYQQAMEEAAWHHMPHPSD
SERIRQYLPRNPCPTPPYHHQMPPPHSDTVEFYQRLSTETLFFIFYYLEGTKAQYLAAKALKKQSWRFHT
KYMMWFQRHEEPKTITDEFEQGTYIYFDYEKWGQRKKEGFTFEYRYLEDRDLQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_014516
ORF Size 2259 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_014516.4
RefSeq Size 2908 bp
RefSeq ORF 2262 bp
Locus ID 4849
UniProt ID O75175
Cytogenetics 19q13.42
Protein Families Transcription Factors
Protein Pathways RNA degradation
MW 81.9 kDa
Gene Summary Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. May be involved in metabolic regulation; may be involved in recruitment of the CCR4-NOT complex to deadenylation target mRNAs involved in energy metabolism. Involved in mitotic progression and regulation of the spindle assembly checkpoint by regulating the stability of MAD1L1 mRNA. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may involve histone deacetylases. Involved in the maintenance of embryonic stem (ES) cell identity.[UniProtKB/Swiss-Prot Function]

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