TOX1 (TOX) (NM_014729) Human Tagged ORF Clone

CAT#: RC203792

TOX (Myc-DDK-tagged)-Human thymocyte selection-associated high mobility group box (TOX)

Product Images

Western blot validation of overexpression lysate (Cat# LY415101) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203792 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TOX protein (Cat# TP303792). The protein was produced from HEK293T cells transfected with TOX cDNA clone (Cat# RC203792) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TOX1
• Synonyms: TOX1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203792 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGTAAGATTTTATCCACCTGTAGCCCAGCCCGCCGCTGCGCCCGACGCTCCCTGTCTGGGACCTT
  CTCCCTGCCTGGACCCCTACTATTGCAACAAGTTTGACGGTGAGAACATGTATATGAGCATGACAGAGCC
  GAGCCAGGACTATGTGCCAGCCAGCCAGTCCTACCCTGGTCCAAGCCTGGAAAGTGAAGACTTCAACATT
  CCACCAATTACTCCTCCTTCCCTCCCAGACCACTCGCTGGTGCACCTGAATGAAGTTGAGTCTGGTTACC
  ATTCTCTGTGTCACCCCATGAACCATAATGGCCTGCTACCATTTCATCCACAAAACATGGACCTCCCTGA
  AATCACAGTCTCCAATATGCTGGGCCAGGATGGAACACTGCTTTCTAATTCCATTTCTGTGATGCCAGAT
  ATACGAAACCCAGAAGGAACTCAGTACAGTTCCCATCCTCAGATGGCAGCCATGAGACCAAGGGGCCAGC
  CTGCAGACATCAGGCAGCAGCCAGGAATGATGCCACATGGCCAGCTGACTACCATTAACCAGTCACAGCT
  AAGTGCTCAACTTGGTTTGAATATGGGAGGAAGCAATGTTCCCCACAACTCACCATCTCCACCTGGAAGC
  AAGTCTGCAACTCCTTCACCATCCAGTTCAGTGCATGAAGATGAAGGCGATGATACCTCTAAGATCAATG
  GTGGAGAGAAGCGGCCTGCCTCTGATATGGGGAAAAAACCAAAAACTCCCAAAAAGAAGAAGAAGAAGGA
  TCCCAATGAGCCCCAGAAGCCTGTGTCTGCCTATGCGTTATTCTTTCGTGATACTCAGGCCGCCATCAAG
  GGCCAAAATCCAAACGCTACCTTTGGCGAAGTCTCTAAAATTGTGGCTTCAATGTGGGACGGTTTAGGAG
  AAGAGCAAAAACAGGTCTATAAAAAGAAAACCGAGGCTGCGAAGAAGGAGTACCTGAAGCAACTCGCAGC
  ATACAGAGCCAGCCTTGTATCCAAGAGCTACAGTGAACCTGTTGACGTGAAGACATCTCAACCTCCTCAG
  CTGATCAATTCGAAGCCGTCGGTGTTCCATGGGCCCAGCCAGGCCCACTCGGCCCTGTACCTAAGTTCCC
  ACTATCACCAACAACCGGGAATGAATCCTCACCTAACTGCCATGCATCCTAGTCTCCCCAGGAACATAGC
  CCCCAAGCCGAATAACCAAATGCCAGTGACTGTCTCTATAGCAAACATGGCTGTGTCCCCTCCTCCTCCC
  CTCCAGATCAGCCCGCCTCTTCACCAGCATCTCAACATGCAGCAGCACCAGCCGCTCACCATGCAGCAGC
  CCCTTGGGAACCAGCTCCCCATGCAGGTCCAGTCTGCCTTACACTCACCCACCATGCAGCAAGGATTTAC
  TCTTCAACCCGACTATCAGACTATTATCAATCCTACATCTACAGCTGCACAAGTTGTCACCCAGGCAATG
  GAGTATGTGCGTTCGGGGTGCAGAAATCCTCCCCCACAACCGGTGGACTGGAATAACGACTACTGCAGTA
  GTGGGGGCATGCAGAGGGACAAAGCACTGTACCTTACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203792 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDVRFYPPVAQPAAAPDAPCLGPSPCLDPYYCNKFDGENMYMSMTEPSQDYVPASQSYPGPSLESEDFNI
  PPITPPSLPDHSLVHLNEVESGYHSLCHPMNHNGLLPFHPQNMDLPEITVSNMLGQDGTLLSNSISVMPD
  IRNPEGTQYSSHPQMAAMRPRGQPADIRQQPGMMPHGQLTTINQSQLSAQLGLNMGGSNVPHNSPSPPGS
  KSATPSPSSSVHEDEGDDTSKINGGEKRPASDMGKKPKTPKKKKKKDPNEPQKPVSAYALFFRDTQAAIK
  GQNPNATFGEVSKIVASMWDGLGEEQKQVYKKKTEAAKKEYLKQLAAYRASLVSKSYSEPVDVKTSQPPQ
  LINSKPSVFHGPSQAHSALYLSSHYHQQPGMNPHLTAMHPSLPRNIAPKPNNQMPVTVSIANMAVSPPPP
  LQISPPLHQHLNMQQHQPLTMQQPLGNQLPMQVQSALHSPTMQQGFTLQPDYQTIINPTSTAAQVVTQAM
  EYVRSGCRNPPPQPVDWNNDYCSSGGMQRDKALYLT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014729
• ORF Size: 1578 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014729.3
• RefSeq Size: 4131 bp
• RefSeq ORF: 1581 bp
• Locus ID: 9760
• UniProt ID: O94900
• Cytogenetics: 8q12.1
• Domains: HMG
• Protein Families: Transcription Factors
• MW: 57.5 kDa
• Gene Summary: The protein encoded by this gene contains a HMG box DNA binding domain. HMG boxes are found in many eukaryotic proteins involved in chromatin assembly, transcription and replication. This protein may function to regulate T-cell development.[provided by RefSeq, Apr 2009]