OLFM2 (NM_058164) Human Tagged ORF Clone

CAT#: RC203786

OLFM2 (Myc-DDK-tagged)-Human olfactomedin 2 (OLFM2)

Product Images

Circular map for RC203786

• 

  Western blot validation of overexpression lysate (Cat# LY403325) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203786 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OLFM2
• Synonyms: NOE2; NOELIN2; NOELIN2_V1; OlfC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203786 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCCGCTCACGGTCCCGCCGCCGCTGCTGCTGCTGCTGTGCTCAGGCCTGGCCGGACAGACTCTCT
  TCCAGAACCCAGAAGAGGGCTGGCAGCTGTACACCTCAGCCCAGGCCCCTGACGGGAAATGCATCTGCAC
  GGCCGTGATCCCAGCGCAGAGTACCTGCTCTCGAGATGGCAGGAGTCGGGAGCTGCGGCAACTGATGGAG
  AAGGTCCAGAACGTCTCCCAGTCCATGGAGGTCCTTGAGTTGCGGACGTATCGCGACCTCCAGTATGTAC
  GCGGCATGGAGACCCTCATGCGGAGCCTGGATGCGCGGCTCCGGGCAGCTGATGGGTCCCTCTCGGCCAA
  GAGCTTCCAGGAGCTGAAGGACAGGATGACGGAACTGTTGCCCCTGAGCTCGGTCCTGGAGCAGTACAAG
  GCAGACACGCGGACCATTGTACGCTTGCGGGAGGAGGTGAGGAATCTCTCCGGCAGTCTGGCGGCCATTC
  AGGAGGAGATGGGTGCCTACGGGTATGAGGACCTGCAGCAACGGGTGATGGCCCTGGAGGCCCGGCTCCA
  CGCCTGCGCCCAGAAGCTGGGCTGTGGGAAGCTGACCGGGGTCAGTAACCCCATCACCGTTCGGGCCATG
  GGGTCCCGCTTCGGCTCCTGGATGACTGACACGATGGCCCCCAGTGCGGATAGCCGGGTCTGGTACATGG
  ATGGCTATTACAAAGGCCGCCGGGTCCTGGAGTTCCGTACCCTGGGAGACTTCATCAAAGGCCAGAACTT
  TATCCAGCACCTGCTGCCCCAGCCGTGGGCGGGCACGGGCCACGTGGTGTACAACGGCTCCCTGTTCTAT
  AACAAGTACCAGAGCAACGTGGTGGTCAAATACCACTTCCGCTCGCGCTCTGTGCTGGTGCAGAGGAGCC
  TCCCGGGCGCCGGTTACAACAACACCTTCCCCTACTCCTGGGGCGGCTTCTCCGACATGGACTTCATGGT
  GGACGAGAGCGGGCTCTGGGCTGTGTACACCACCAACCAGAACGCGGGCAACATCGTGGTCAGCCGGCTG
  GACCCGCACACCCTCGAGGTCATGCGGTCCTGGGACACCGGCTACCCCAAGCGCAGCGCTGGCGAGGCCT
  TCATGATCTGCGGTGTGCTCTACGTGACCAACTCCCACCTGGCTGGGGCCAAGGTCTACTTCGCCTATTT
  TACCAACACGTCCAGTTACGAGTACACGGACGTGCCCTTCCACAACCAGTATTCCCACATCTCGATGCTG
  GATTACAACCCCCGGGAGCGCGCCCTCTATACCTGGAACAACGGCCACCAGGTGCTCTACAATGTCACCC
  TGTTTCACGTCATCAGCACCTCTGGGGACCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203786 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MWPLTVPPPLLLLLCSGLAGQTLFQNPEEGWQLYTSAQAPDGKCICTAVIPAQSTCSRDGRSRELRQLME
  KVQNVSQSMEVLELRTYRDLQYVRGMETLMRSLDARLRAADGSLSAKSFQELKDRMTELLPLSSVLEQYK
  ADTRTIVRLREEVRNLSGSLAAIQEEMGAYGYEDLQQRVMALEARLHACAQKLGCGKLTGVSNPITVRAM
  GSRFGSWMTDTMAPSADSRVWYMDGYYKGRRVLEFRTLGDFIKGQNFIQHLLPQPWAGTGHVVYNGSLFY
  NKYQSNVVVKYHFRSRSVLVQRSLPGAGYNNTFPYSWGGFSDMDFMVDESGLWAVYTTNQNAGNIVVSRL
  DPHTLEVMRSWDTGYPKRSAGEAFMICGVLYVTNSHLAGAKVYFAYFTNTSSYEYTDVPFHNQYSHISML
  DYNPRERALYTWNNGHQVLYNVTLFHVISTSGDP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_058164
• ORF Size: 1362 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_058164.4
• RefSeq Size: 1899 bp
• RefSeq ORF: 1365 bp
• Locus ID: 93145
• UniProt ID: O95897
• Cytogenetics: 19p13.2
• Domains: OLF
• Protein Families: Druggable Genome, Secreted Protein
• MW: 51.4 kDa
• Gene Summary: Involved in transforming growth factor beta (TGF-beta)-induced smooth muscle differentiation. TGF-beta induces expression and translocation of OLFM2 to the nucleus where it binds to SRF, causing its dissociation from the transcriptional repressor HEY2/HERP1 and facilitating binding of SRF to target genes (PubMed:25298399). Plays a role in AMPAR complex organization (By similarity). Is a regulator of vascular smooth-muscle cell (SMC) phenotypic switching, that acts by promoting RUNX2 and inhibiting MYOCD binding to SRF. SMC phenotypic switching is the process through which vascular SMCs undergo transition between a quiescent contractile phenotype and a proliferative synthetic phenotype in response to pathological stimuli. SMC phenotypic plasticity is essential for vascular development and remodeling (By similarity).[UniProtKB/Swiss-Prot Function]