SMRC2 (SMARCC2) (NM_139067) Human Tagged ORF Clone

CAT#: RC203774

SMARCC2 (Myc-DDK-tagged)-Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2 (SMARCC2), transcript variant 2

Product Images

Circular map for RC203774

• 

  Western blot validation of overexpression lysate (Cat# LY408410) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203774 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified SMARCC2 protein (Cat# TP303774). The protein was produced from HEK293T cells transfected with SMARCC2 cDNA clone (Cat# RC203774) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SMRC2
• Synonyms: BAF170; CRACC2; CSS8; Rsc8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203774 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTGCGGAAGAAGGACGGCGGCCCCAACGTGAAGTACTACGAGGCCGCGGACACCGTGACCCAGT
  TCGACAACGTGCGGCTGTGGCTCGGCAAGAACTACAAGAAGTATATACAAGCTGAACCACCCACCAACAA
  GTCCCTGTCTAGCCTGGTTGTACAGTTGCTACAATTTCAGGAAGAAGTTTTTGGCAAACATGTCAGCAAT
  GCACCGCTCACTAAACTGCCGATCAAATGTTTCCTAGATTTCAAAGCGGGAGGCTCCTTGTGCCACATTC
  TTGCAGCTGCCTACAAATTCAAGAGTGACCAGGGATGGCGGCGTTACGATTTCCAGAATCCATCACGCAT
  GGACCGCAATGTGGAAATGTTTATGACCATTGAGAAGTCCTTGGTGCAGAATAATTGCCTGTCTCGACCT
  AACATTTTTCTGTGCCCAGAAATTGAGCCCAAACTACTAGGGAAATTAAAGGACATTATCAAGAGACACC
  AGGGAACAGTCACTGAGGATAAGAACAATGCCTCCCATGTTGTGTATCCTGTCCCGGGGAATCTAGAAGA
  AGAGGAATGGGTACGACCAGTCATGAAGAGGGATAAGCAGGTTCTTCTGCACTGGGGCTACTATCCTGAC
  AGTTACGACACGTGGATCCCAGCGAGTGAAATTGAGGCATCTGTGGAAGATGCTCCAACTCCTGAGAAAC
  CTAGGAAGGTTCATGCAAAGTGGATCCTGGACACCGACACCTTCAATGAATGGATGAATGAGGAAGACTA
  TGAAGTAAATGATGACAAAAACCCTGTCTCCCGCCGAAAGAAGATTTCAGCCAAGACACTGACAGATGAG
  GTGAACAGCCCAGATTCAGATCGACGGGACAAGAAGGGGGGAAACTATAAGAAGAGGAAGCGCTCCCCCT
  CTCCTTCACCAACCCCAGAAGCAAAGAAGAAAAATGCTAAGAAAGGTCCCTCAACACCTTACACTAAGTC
  AAAGCGTGGCCACAGAGAAGAGGAGCAAGAAGACCTGACAAAGGACATGGACGAGCCCTCACCAGTCCCC
  AATGTAGAAGAGGTGACACTTCCCAAAACAGTCAACACAAAGAAAGACTCAGAGTCGGCCCCAGTCAAAG
  GCGGCACCATGACCGACCTGGATGAACAGGAAGATGAAAGCATGGAGACGACGGGCAAGGATGAGGATGA
  GAACAGTACGGGGAACAAGGGAGAGCAGACCAAGAATCCAGACCTGCATGAGGACAATGTGACTGAACAG
  ACCCACCACATCATCATTCCCAGCTACGCTGCCTGGTTTGACTACAATAGTGTTCATGCCATTGAGCGGA
  GGGCTCTCCCCGAGTTCTTCAACGGCAAGAACAAGTCCAAGACTCCAGAGATCTACCTGGCCTATCGAAA
  CTTTATGATTGACACTTACCGACTGAACCCCCAAGAGTATCTTACCTCTACCGCCTGCCGCCGAAACCTA
  GCGGGTGATGTCTGTGCCATCATGAGGGTCCATGCCTTCCTAGAACAGTGGGGTCTTATTAACTACCAGG
  TGGATGCTGAGAGTCGACCAACCCCAATGGGGCCTCCGCCTACCTCTCACTTCCATGTCTTGGCTGACAC
  ACCATCAGGGCTGGTGCCTCTGCAGCCCAAGACACCTCAGGGCCGCCAGGTTGATGCTGATACCAAGGCT
  GGGCGAAAGGGCAAAGAGCTGGATGACCTGGTGCCAGAGACGGCTAAGGGCAAGCCAGAGCTGCAGACCT
  CTGCTTCCCAACAAATGCTCAACTTTCCTGACAAAGGCAAAGAGAAACCAACAGACATGCAAAACTTTGG
  GCTGCGCACAGACATGTACACAAAAAAGAATGTTCCCTCCAAGAGCAAGGCTGCAGCCAGTGCCACTCGT
  GAGTGGACAGAACAGGAAACCCTGCTTCTCCTGGAGGCACTGGAAATGTACAAAGATGACTGGAACAAAG
  TGTCCGAGCATGTGGGAAGCCGCACACAGGACGAGTGCATCTTGCATTTTCTTCGTCTTCCCATTGAAGA
  CCCATACCTGGAGGACTCAGAGGCCTCCCTAGGCCCCCTGGCCTACCAACCCATCCCCTTCAGTCAGTCG
  GGCAACCCTGTTATGAGCACTGTTGCCTTCCTGGCCTCTGTCGTCGATCCCCGAGTCGCCTCTGCTGCTG
  CAAAGTCAGCCCTAGAGGAGTTCTCCAAAATGAAGGAAGAGGTACCCACGGCCTTGGTGGAGGCCCATGT
  TCGAAAAGTGGAAGAAGCAGCCAAAGTAACAGGCAAGGCGGACCCTGCCTTCGGTCTGGAAAGCAGTGGC
  ATTGCAGGAACCACCTCTGATGAGCCTGAGCGGATTGAGGAGAGCGGGAATGACGAGGCTCGGGTGGAAG
  GCCAGGCCACAGATGAGAAGAAGGAGCCCAAGGAACCCCGAGAAGGAGGGGGTGCTATAGAGGAGGAAGC
  AAAAGAGAAAACCAGCGAGGCTCCCAAGAAGGATGAGGAGAAAGGGAAAGAAGGCGACAGTGAGAAGGAG
  TCCGAGAAGAGTGATGGAGACCCAATAGTCGATCCTGAGAAGGAGAAGGAGCCAAAGGAAGGGCAGGAGG
  AAGTGCTGAAGGAAGTGGTGGAGTCTGAGGGGGAAAGGAAGACAAAGGTGGAGCGGGACATTGGCGAGGG
  CAACCTCTCCACCGCTGCTGCCGCCGCCCTGGCCGCCGCCGCAGTGAAAGCTAAGCACTTTGCTGCTGTT
  GAGGAAAGGAAGATCAAATCTTTGGTGGCCCTGCTGGTGGAGACCCAGATGAAAAAGTTGGAGATCAAAC
  TTCGGCACTTTGAGGAGCTGGAGACTATCATGGACCGGGAGCGAGAAGCACTGGAGTATCAGAGGCAGCA
  GCTCCTGGCCGACAGACAAGCCTTCCACATGGAGCAGCTGAAGTATGCGGAGATGAGGGCTCGGCAGCAG
  CACTTCCAACAGATGCACCAACAGCAGCAGCAGCCACCACCAGCCCTGCCCCCAGGCTCCCAGCCTATCC
  CCCCAACAGGGGCTGCTGGGCCACCCGCAGTCCATGGCTTGGCTGTGGCTCCAGCCTCTGTAGTCCCTGC
  TCCTGCTGGCAGTGGGGCCCCTCCAGGAAGTTTGGGCCCTTCTGAACAGATTGGGCAGGCAGGGTCAACT
  GCAGGGCCACAGCAGCAGCAACCAGCTGGAGCCCCCCAGCCTGGGGCAGTCCCACCAGGGGTTCCCCCCC
  CTGGACCCCATGGCCCCTCACCGTTCCCCAACCAACAAACTCCTCCCTCAATGATGCCAGGGGCAGTGCC
  AGGCAGCGGGCACCCAGGCGTGGCGGACCCAGGCACCCCCCTGCCTCCAGACCCCACAGCCCCGAGCCCA
  GGCACGGTCACCCCTGTGCCACCTCCACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203774 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAVRKKDGGPNVKYYEAADTVTQFDNVRLWLGKNYKKYIQAEPPTNKSLSSLVVQLLQFQEEVFGKHVSN
  APLTKLPIKCFLDFKAGGSLCHILAAAYKFKSDQGWRRYDFQNPSRMDRNVEMFMTIEKSLVQNNCLSRP
  NIFLCPEIEPKLLGKLKDIIKRHQGTVTEDKNNASHVVYPVPGNLEEEEWVRPVMKRDKQVLLHWGYYPD
  SYDTWIPASEIEASVEDAPTPEKPRKVHAKWILDTDTFNEWMNEEDYEVNDDKNPVSRRKKISAKTLTDE
  VNSPDSDRRDKKGGNYKKRKRSPSPSPTPEAKKKNAKKGPSTPYTKSKRGHREEEQEDLTKDMDEPSPVP
  NVEEVTLPKTVNTKKDSESAPVKGGTMTDLDEQEDESMETTGKDEDENSTGNKGEQTKNPDLHEDNVTEQ
  THHIIIPSYAAWFDYNSVHAIERRALPEFFNGKNKSKTPEIYLAYRNFMIDTYRLNPQEYLTSTACRRNL
  AGDVCAIMRVHAFLEQWGLINYQVDAESRPTPMGPPPTSHFHVLADTPSGLVPLQPKTPQGRQVDADTKA
  GRKGKELDDLVPETAKGKPELQTSASQQMLNFPDKGKEKPTDMQNFGLRTDMYTKKNVPSKSKAAASATR
  EWTEQETLLLLEALEMYKDDWNKVSEHVGSRTQDECILHFLRLPIEDPYLEDSEASLGPLAYQPIPFSQS
  GNPVMSTVAFLASVVDPRVASAAAKSALEEFSKMKEEVPTALVEAHVRKVEEAAKVTGKADPAFGLESSG
  IAGTTSDEPERIEESGNDEARVEGQATDEKKEPKEPREGGGAIEEEAKEKTSEAPKKDEEKGKEGDSEKE
  SEKSDGDPIVDPEKEKEPKEGQEEVLKEVVESEGERKTKVERDIGEGNLSTAAAAALAAAAVKAKHFAAV
  EERKIKSLVALLVETQMKKLEIKLRHFEELETIMDREREALEYQRQQLLADRQAFHMEQLKYAEMRARQQ
  HFQQMHQQQQQPPPALPPGSQPIPPTGAAGPPAVHGLAVAPASVVPAPAGSGAPPGSLGPSEQIGQAGST
  AGPQQQQPAGAPQPGAVPPGVPPPGPHGPSPFPNQQTPPSMMPGAVPGSGHPGVADPGTPLPPDPTAPSP
  GTVTPVPPPQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_139067
• ORF Size: 3390 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_139067.4
• RefSeq Size: 5336 bp
• RefSeq ORF: 3393 bp
• Locus ID: 6601
• UniProt ID: Q8TAQ2
• Cytogenetics: 12q13.2
• Domains: CHROMO, myb_DNA-binding, SWIRM
• Protein Families: Transcription Factors
• MW: 124.9 kDa
• Gene Summary: The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]