SMRC2 (SMARCC2) (NM_139067) Human Tagged ORF Clone

CAT#: RC203774

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SMARCC2 (Myc-DDK-tagged)-Human SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2 (SMARCC2), transcript variant 2



  "NM_139067" in other vectors (4)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 118.00

USD 880.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol SMARCC2
Synonyms BAF170; CRACC2; CSS8; Rsc8
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC203774 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGTGCGGAAGAAGGACGGCGGCCCCAACGTGAAGTACTACGAGGCCGCGGACACCGTGACCCAGT
TCGACAACGTGCGGCTGTGGCTCGGCAAGAACTACAAGAAGTATATACAAGCTGAACCACCCACCAACAA
GTCCCTGTCTAGCCTGGTTGTACAGTTGCTACAATTTCAGGAAGAAGTTTTTGGCAAACATGTCAGCAAT
GCACCGCTCACTAAACTGCCGATCAAATGTTTCCTAGATTTCAAAGCGGGAGGCTCCTTGTGCCACATTC
TTGCAGCTGCCTACAAATTCAAGAGTGACCAGGGATGGCGGCGTTACGATTTCCAGAATCCATCACGCAT
GGACCGCAATGTGGAAATGTTTATGACCATTGAGAAGTCCTTGGTGCAGAATAATTGCCTGTCTCGACCT
AACATTTTTCTGTGCCCAGAAATTGAGCCCAAACTACTAGGGAAATTAAAGGACATTATCAAGAGACACC
AGGGAACAGTCACTGAGGATAAGAACAATGCCTCCCATGTTGTGTATCCTGTCCCGGGGAATCTAGAAGA
AGAGGAATGGGTACGACCAGTCATGAAGAGGGATAAGCAGGTTCTTCTGCACTGGGGCTACTATCCTGAC
AGTTACGACACGTGGATCCCAGCGAGTGAAATTGAGGCATCTGTGGAAGATGCTCCAACTCCTGAGAAAC
CTAGGAAGGTTCATGCAAAGTGGATCCTGGACACCGACACCTTCAATGAATGGATGAATGAGGAAGACTA
TGAAGTAAATGATGACAAAAACCCTGTCTCCCGCCGAAAGAAGATTTCAGCCAAGACACTGACAGATGAG
GTGAACAGCCCAGATTCAGATCGACGGGACAAGAAGGGGGGAAACTATAAGAAGAGGAAGCGCTCCCCCT
CTCCTTCACCAACCCCAGAAGCAAAGAAGAAAAATGCTAAGAAAGGTCCCTCAACACCTTACACTAAGTC
AAAGCGTGGCCACAGAGAAGAGGAGCAAGAAGACCTGACAAAGGACATGGACGAGCCCTCACCAGTCCCC
AATGTAGAAGAGGTGACACTTCCCAAAACAGTCAACACAAAGAAAGACTCAGAGTCGGCCCCAGTCAAAG
GCGGCACCATGACCGACCTGGATGAACAGGAAGATGAAAGCATGGAGACGACGGGCAAGGATGAGGATGA
GAACAGTACGGGGAACAAGGGAGAGCAGACCAAGAATCCAGACCTGCATGAGGACAATGTGACTGAACAG
ACCCACCACATCATCATTCCCAGCTACGCTGCCTGGTTTGACTACAATAGTGTTCATGCCATTGAGCGGA
GGGCTCTCCCCGAGTTCTTCAACGGCAAGAACAAGTCCAAGACTCCAGAGATCTACCTGGCCTATCGAAA
CTTTATGATTGACACTTACCGACTGAACCCCCAAGAGTATCTTACCTCTACCGCCTGCCGCCGAAACCTA
GCGGGTGATGTCTGTGCCATCATGAGGGTCCATGCCTTCCTAGAACAGTGGGGTCTTATTAACTACCAGG
TGGATGCTGAGAGTCGACCAACCCCAATGGGGCCTCCGCCTACCTCTCACTTCCATGTCTTGGCTGACAC
ACCATCAGGGCTGGTGCCTCTGCAGCCCAAGACACCTCAGGGCCGCCAGGTTGATGCTGATACCAAGGCT
GGGCGAAAGGGCAAAGAGCTGGATGACCTGGTGCCAGAGACGGCTAAGGGCAAGCCAGAGCTGCAGACCT
CTGCTTCCCAACAAATGCTCAACTTTCCTGACAAAGGCAAAGAGAAACCAACAGACATGCAAAACTTTGG
GCTGCGCACAGACATGTACACAAAAAAGAATGTTCCCTCCAAGAGCAAGGCTGCAGCCAGTGCCACTCGT
GAGTGGACAGAACAGGAAACCCTGCTTCTCCTGGAGGCACTGGAAATGTACAAAGATGACTGGAACAAAG
TGTCCGAGCATGTGGGAAGCCGCACACAGGACGAGTGCATCTTGCATTTTCTTCGTCTTCCCATTGAAGA
CCCATACCTGGAGGACTCAGAGGCCTCCCTAGGCCCCCTGGCCTACCAACCCATCCCCTTCAGTCAGTCG
GGCAACCCTGTTATGAGCACTGTTGCCTTCCTGGCCTCTGTCGTCGATCCCCGAGTCGCCTCTGCTGCTG
CAAAGTCAGCCCTAGAGGAGTTCTCCAAAATGAAGGAAGAGGTACCCACGGCCTTGGTGGAGGCCCATGT
TCGAAAAGTGGAAGAAGCAGCCAAAGTAACAGGCAAGGCGGACCCTGCCTTCGGTCTGGAAAGCAGTGGC
ATTGCAGGAACCACCTCTGATGAGCCTGAGCGGATTGAGGAGAGCGGGAATGACGAGGCTCGGGTGGAAG
GCCAGGCCACAGATGAGAAGAAGGAGCCCAAGGAACCCCGAGAAGGAGGGGGTGCTATAGAGGAGGAAGC
AAAAGAGAAAACCAGCGAGGCTCCCAAGAAGGATGAGGAGAAAGGGAAAGAAGGCGACAGTGAGAAGGAG
TCCGAGAAGAGTGATGGAGACCCAATAGTCGATCCTGAGAAGGAGAAGGAGCCAAAGGAAGGGCAGGAGG
AAGTGCTGAAGGAAGTGGTGGAGTCTGAGGGGGAAAGGAAGACAAAGGTGGAGCGGGACATTGGCGAGGG
CAACCTCTCCACCGCTGCTGCCGCCGCCCTGGCCGCCGCCGCAGTGAAAGCTAAGCACTTTGCTGCTGTT
GAGGAAAGGAAGATCAAATCTTTGGTGGCCCTGCTGGTGGAGACCCAGATGAAAAAGTTGGAGATCAAAC
TTCGGCACTTTGAGGAGCTGGAGACTATCATGGACCGGGAGCGAGAAGCACTGGAGTATCAGAGGCAGCA
GCTCCTGGCCGACAGACAAGCCTTCCACATGGAGCAGCTGAAGTATGCGGAGATGAGGGCTCGGCAGCAG
CACTTCCAACAGATGCACCAACAGCAGCAGCAGCCACCACCAGCCCTGCCCCCAGGCTCCCAGCCTATCC
CCCCAACAGGGGCTGCTGGGCCACCCGCAGTCCATGGCTTGGCTGTGGCTCCAGCCTCTGTAGTCCCTGC
TCCTGCTGGCAGTGGGGCCCCTCCAGGAAGTTTGGGCCCTTCTGAACAGATTGGGCAGGCAGGGTCAACT
GCAGGGCCACAGCAGCAGCAACCAGCTGGAGCCCCCCAGCCTGGGGCAGTCCCACCAGGGGTTCCCCCCC
CTGGACCCCATGGCCCCTCACCGTTCCCCAACCAACAAACTCCTCCCTCAATGATGCCAGGGGCAGTGCC
AGGCAGCGGGCACCCAGGCGTGGCGGACCCAGGCACCCCCCTGCCTCCAGACCCCACAGCCCCGAGCCCA
GGCACGGTCACCCCTGTGCCACCTCCACAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC203774 protein sequence
Red=Cloning site Green=Tags(s)

MAVRKKDGGPNVKYYEAADTVTQFDNVRLWLGKNYKKYIQAEPPTNKSLSSLVVQLLQFQEEVFGKHVSN
APLTKLPIKCFLDFKAGGSLCHILAAAYKFKSDQGWRRYDFQNPSRMDRNVEMFMTIEKSLVQNNCLSRP
NIFLCPEIEPKLLGKLKDIIKRHQGTVTEDKNNASHVVYPVPGNLEEEEWVRPVMKRDKQVLLHWGYYPD
SYDTWIPASEIEASVEDAPTPEKPRKVHAKWILDTDTFNEWMNEEDYEVNDDKNPVSRRKKISAKTLTDE
VNSPDSDRRDKKGGNYKKRKRSPSPSPTPEAKKKNAKKGPSTPYTKSKRGHREEEQEDLTKDMDEPSPVP
NVEEVTLPKTVNTKKDSESAPVKGGTMTDLDEQEDESMETTGKDEDENSTGNKGEQTKNPDLHEDNVTEQ
THHIIIPSYAAWFDYNSVHAIERRALPEFFNGKNKSKTPEIYLAYRNFMIDTYRLNPQEYLTSTACRRNL
AGDVCAIMRVHAFLEQWGLINYQVDAESRPTPMGPPPTSHFHVLADTPSGLVPLQPKTPQGRQVDADTKA
GRKGKELDDLVPETAKGKPELQTSASQQMLNFPDKGKEKPTDMQNFGLRTDMYTKKNVPSKSKAAASATR
EWTEQETLLLLEALEMYKDDWNKVSEHVGSRTQDECILHFLRLPIEDPYLEDSEASLGPLAYQPIPFSQS
GNPVMSTVAFLASVVDPRVASAAAKSALEEFSKMKEEVPTALVEAHVRKVEEAAKVTGKADPAFGLESSG
IAGTTSDEPERIEESGNDEARVEGQATDEKKEPKEPREGGGAIEEEAKEKTSEAPKKDEEKGKEGDSEKE
SEKSDGDPIVDPEKEKEPKEGQEEVLKEVVESEGERKTKVERDIGEGNLSTAAAAALAAAAVKAKHFAAV
EERKIKSLVALLVETQMKKLEIKLRHFEELETIMDREREALEYQRQQLLADRQAFHMEQLKYAEMRARQQ
HFQQMHQQQQQPPPALPPGSQPIPPTGAAGPPAVHGLAVAPASVVPAPAGSGAPPGSLGPSEQIGQAGST
AGPQQQQPAGAPQPGAVPPGVPPPGPHGPSPFPNQQTPPSMMPGAVPGSGHPGVADPGTPLPPDPTAPSP
GTVTPVPPPQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_139067
ORF Size 3390 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_139067.4
RefSeq Size 5336 bp
RefSeq ORF 3393 bp
Locus ID 6601
UniProt ID Q8TAQ2
Cytogenetics 12q13.2
Domains CHROMO, myb_DNA-binding, SWIRM
Protein Families Transcription Factors
MW 124.9 kDa
Gene Summary The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

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