LCAT (NM_000229) Human Tagged Lenti ORF Clone

CAT#: RC203763L4

  • LentiORF®

Lenti ORF clone of Human lecithin-cholesterol acyltransferase (LCAT), mGFP tagged


  "NM_000229" in other vectors (7)

Reconstitution Protocol

USD 757.00

In Stock*

Size
    • 10 ug

Product Images

Frequently bought together (5)
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Forward primer to sequence targets cloned in expression vectors.
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag mGFP
Symbol LCAT
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
The ORF insert of this clone is exactly the same as(RC203763).
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_000229
ORF Size 1320 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000229.1
RefSeq Size 1354 bp
RefSeq ORF 1323 bp
Locus ID 3931
UniProt ID P04180
Cytogenetics 16q22.1
Domains LACT
Protein Families Druggable Genome, Transmembrane
Protein Pathways Glycerophospholipid metabolism
MW 49.6 kDa
Gene Summary This gene encodes the extracellular cholesterol esterifying enzyme, lecithin-cholesterol acyltransferase. The esterification of cholesterol is required for cholesterol transport. Mutations in this gene have been found to cause fish-eye disease as well as LCAT deficiency. [provided by RefSeq, Jul 2008]

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.