MRAP2 (NM_138409) Human Tagged ORF Clone

CAT#: RC203668

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MRAP2 (Myc-DDK-tagged)-Human melanocortin 2 receptor accessory protein 2 (MRAP2)



  "NM_138409" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 300.00

USD 450.00


Availability*
In Stock

Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol MRAP2
Synonyms bA51G5.2; C6orf117
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC203668 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCGCCCAGAGGTTAATTTCTAACAGAACCTCCCAGCAATCGGCATCTAATTCTGATTACACCTGGG
AATATGAATATTATGAGATTGGACCAGTTTCCTTTGAAGGACTGAAGGCTCATAAATATTCCATTGTGAT
TGGATTTTGGGTTGGTCTTGCAGTCTTCGTGATTTTTATGTTTTTTGTGCTGACCTTGCTGACCAAGACA
GGAGCCCCACACCAAGACAATGCAGAGTCCTCAGAGAAGAGATTCAGAATGAACAGCTTTGTGTCAGACT
TTGGAAGACCTCTGGAGCCAGATAAAGTATTTTCTCGCCAAGGCAACGAGGAGTCCAGGTCTCTCTTTCA
CTGCTACATCAATGAGGTGGAACGCTTGGACAGAGCCAAAGCTTGTCACCAGACCACAGCCCTTGACAGT
GACGTCCAACTCCAGGAAGCCATCAGAAGCAGTGGGCAGCCAGAGGAGGAGCTGAACAGGCTCATGAAGT
TTGACATCCCCAACTTTGTGAACACAGACCAGAACTACTTTGGGGAGGATGATCTTCTGATTTCTGAACC
ACCTATTGTTCTGGAAACTAAGCCACTTTCCCAGACCTCACACAAAGACCTGGAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC203668 protein sequence
Red=Cloning site Green=Tags(s)

MSAQRLISNRTSQQSASNSDYTWEYEYYEIGPVSFEGLKAHKYSIVIGFWVGLAVFVIFMFFVLTLLTKT
GAPHQDNAESSEKRFRMNSFVSDFGRPLEPDKVFSRQGNEESRSLFHCYINEVERLDRAKACHQTTALDS
DVQLQEAIRSSGQPEEELNRLMKFDIPNFVNTDQNYFGEDDLLISEPPIVLETKPLSQTSHKDLD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_138409
ORF Size 615 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_138409.4
RefSeq Size 2229 bp
RefSeq ORF 618 bp
Locus ID 112609
UniProt ID Q96G30
Cytogenetics 6q14.2
Protein Families Transmembrane
MW 23.5 kDa
Gene Summary This gene encodes a protein that modulates melanocortin receptor signaling. The encoded protein has been shown to interact with all known melanocortin receptors and may regulate both receptor trafficking and activation in response to ligands. Mice lacking a functional copy of this gene exhibit severe obesity and a mutation in this gene may be associated with severe obesity in human patients. [provided by RefSeq, Oct 2016]

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