ACTC1 (NM_005159) Human Tagged ORF Clone
Lenti ORF clone of Human actin, alpha, cardiac muscle 1 (ACTC1), mGFP tagged
|Product Name||ACTC1 (NM_005159) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||ACTC; ASD5; CMD1R; CMH11; LVNC4|
|E. coli Selection||Chloramphenicol (34 ug/mL)|
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene|
|ORF Size||1131 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||3693 bp|
|RefSeq ORF||1134 bp|
|Protein Pathways||Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM)|
|Gene Summary||Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). [provided by RefSeq, Jul 2008]|
|cDNA Clone Resources|
|RC203655L3||Lenti ORF clone of Human actin, alpha, cardiac muscle 1 (ACTC1), Myc-DDK-tagged||
|RG203655||ACTC1 (GFP-tagged) - Human actin, alpha, cardiac muscle 1 (ACTC1)||
|RC203655||ACTC1 (Myc-DDK-tagged)-Human actin, alpha, cardiac muscle 1 (ACTC1)||
|SC319325||ACTC1 (untagged)-Human actin, alpha, cardiac muscle 1 (ACTC1)||