LRRC8D (NM_018103) Human Tagged ORF Clone

CAT#: RC203641

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LRRC8D (Myc-DDK-tagged)-Human leucine rich repeat containing 8 family, member D (LRRC8D), transcript variant 2



  "NM_018103" in other vectors (7)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol LRRC8D
Synonyms LRRC5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC203641 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTACCCTTGCGGAAGTTGCATCACTTAATGACATTCAGCCAACTTACCGAATCCTGAAACCATGGT
GGGATGTGTTTATGGATTACCTAGCTGTTGTTATGTTAATGGTAGCCATCTTTGCAGGAACCATGCAACT
TACCAAAGATCAGGTGGTCTGTTTGCCAGTATTGCCATCTCCTGTAAATTCAAAGGCACATACACCACCA
GGAAATGCCGAGGTCACCACCAACATCCCAAAGATGGAAGCAGCCACCAACCAAGACCAAGATGGGCGGA
CAACAAACGACATTTCCTTTGGGACATCTGCTGTGACACCTGACATACCTCTCAGAGCCACATATCCTCG
CACAGATTTCGCACTTCCAAATCAGGAGGCAAAGAAAGAGAAGAAAGATCCAACAGGTCGAAAAACAAAC
TTGGATTTTCAGCAATATGTATTTATTAATCAAATGTGTTACCATCTGGCCCTTCCGTGGTATTCTAAGT
ACTTTCCATACCTAGCTCTTATACATACTATTATTCTCATGGTCAGTAGCAACTTTTGGTTCAAATATCC
CAAAACATGCTCAAAAGTAGAACATTTTGTTTCAATATTAGGAAAGTGCTTTGAATCCCCTTGGACGACA
AAAGCGTTGTCTGAGACAGCATGCGAAGACTCAGAGGAAAACAAGCAGAGAATAACAGGTGCCCAGACTC
TACCAAAGCATGTTTCTACCAGCAGTGATGAAGGGAGCCCCAGTGCCAGTACACCAATGATCAATAAAAC
TGGCTTTAAATTTTCAGCTGAGAAGCCTGTGATTGAAGTTCCCAGCATGACAATCCTGGATAAAAAGGAT
GGAGAGCAGGCCAAAGCCCTGTTTGAGAAAGTGAGGAAGTTCCGTGCCCATGTGGAAGATAGTGACTTGA
TCTATAAACTCTATGTGGTCCAAACAGTTATCAAAACAGCCAAGTTCATTTTTATTCTCTGCTATACAGC
GAACTTTGTCAACGCAATCAGCTTTGAACACGTCTGCAAGCCCAAAGTTGAGCATCTGATTGGTTATGAG
GTATTTGAGTGCACCCACAATATGGCTTACATGTTGAAAAAGCTTCTCATCAGTTACATATCCATTATTT
GTGTTTATGGCTTTATCTGCCTCTACACTCTCTTCTGGTTATTCAGGATACCTTTGAAGGAATATTCTTT
CGAAAAAGTCAGAGAAGAGAGCAGTTTTAGTGACATTCCAGATGTCAAAAACGATTTTGCGTTCCTTCTT
CACATGGTAGACCAGTATGACCAGCTATATTCCAAGCGTTTTGGTGTGTTCTTGTCAGAAGTTAGTGAAA
ATAAACTTAGGGAAATTAGTTTGAACCATGAGTGGACATTTGAAAAACTCAGGCAGCACATTTCACGCAA
CGCCCAGGACAAGCAGGAGTTGCATCTGTTCATGCTGTCGGGGGTGCCCGATGCTGTCTTTGACCTCACA
GACCTGGATGTGCTAAAGCTTGAACTAATTCCAGAAGCTAAAATTCCTGCTAAGATTTCTCAAATGACTA
ACCTCCAAGAGCTCCACCTCTGCCACTGCCCTGCAAAAGTTGAACAGACTGCTTTTAGCTTTCTTCGCGA
TCACTTGAGATGCCTTCACGTGAAGTTCACTGATGTGGCTGAAATTCCTGCCTGGGTGTATTTGCTCAAA
AACCTTCGAGAGTTGTACTTAATAGGCAATTTGAACTCTGAAAACAATAAGATGATAGGACTTGAATCTC
TCCGAGAGTTGCGGCACCTTAAGATTCTCCACGTGAAGAGCAATTTGACCAAAGTTCCCTCCAACATTAC
AGATGTGGCTCCACATCTTACAAAGTTAGTCATTCATAATGACGGCACTAAACTCTTGGTACTGAACAGC
CTTAAGAAAATGATGAATGTCGCTGAGCTGGAACTCCAGAACTGTGAGCTAGAGAGAATCCCACATGCTA
TTTTCAGCCTCTCTAATTTACAGGAACTGGATTTAAAGTCCAATAACATTCGCACAATTGAGGAAATCAT
CAGTTTCCAGCATTTAAAACGACTGACTTGTTTAAAATTATGGCATAACAAAATTGTTACTATTCCTCCC
TCTATTACCCATGTCAAAAACTTGGAGTCACTTTATTTCTCTAACAACAAGCTCGAATCCTTACCAGTGG
CAGTATTTAGTTTACAGAAACTCAGATGCTTAGATGTGAGCTACAACAACATTTCAATGATTCCAATAGA
AATAGGATTGCTTCAGAACCTGCAGCATTTGCATATCACTGGGAACAAAGTGGACATTCTGCCAAAACAA
TTGTTTAAATGCATAAAGTTGAGGACTTTGAATCTGGGACAGAACTGCATCACCTCACTCCCAGAGAAAG
TTGGTCAGCTCTCCCAGCTCACTCAGCTGGAGCTGAAGGGGAACTGCTTGGACCGCCTGCCAGCCCAGCT
GGGCCAGTGTCGGATGCTCAAGAAAAGCGGGCTTGTTGTGGAAGATCACCTTTTTGATACCCTGCCACTC
GAAGTCAAAGAGGCATTGAATCAAGACATAAATATTCCCTTTGCAAATGGGATT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC203641 protein sequence
Red=Cloning site Green=Tags(s)

MFTLAEVASLNDIQPTYRILKPWWDVFMDYLAVVMLMVAIFAGTMQLTKDQVVCLPVLPSPVNSKAHTPP
GNAEVTTNIPKMEAATNQDQDGRTTNDISFGTSAVTPDIPLRATYPRTDFALPNQEAKKEKKDPTGRKTN
LDFQQYVFINQMCYHLALPWYSKYFPYLALIHTIILMVSSNFWFKYPKTCSKVEHFVSILGKCFESPWTT
KALSETACEDSEENKQRITGAQTLPKHVSTSSDEGSPSASTPMINKTGFKFSAEKPVIEVPSMTILDKKD
GEQAKALFEKVRKFRAHVEDSDLIYKLYVVQTVIKTAKFIFILCYTANFVNAISFEHVCKPKVEHLIGYE
VFECTHNMAYMLKKLLISYISIICVYGFICLYTLFWLFRIPLKEYSFEKVREESSFSDIPDVKNDFAFLL
HMVDQYDQLYSKRFGVFLSEVSENKLREISLNHEWTFEKLRQHISRNAQDKQELHLFMLSGVPDAVFDLT
DLDVLKLELIPEAKIPAKISQMTNLQELHLCHCPAKVEQTAFSFLRDHLRCLHVKFTDVAEIPAWVYLLK
NLRELYLIGNLNSENNKMIGLESLRELRHLKILHVKSNLTKVPSNITDVAPHLTKLVIHNDGTKLLVLNS
LKKMMNVAELELQNCELERIPHAIFSLSNLQELDLKSNNIRTIEEIISFQHLKRLTCLKLWHNKIVTIPP
SITHVKNLESLYFSNNKLESLPVAVFSLQKLRCLDVSYNNISMIPIEIGLLQNLQHLHITGNKVDILPKQ
LFKCIKLRTLNLGQNCITSLPEKVGQLSQLTQLELKGNCLDRLPAQLGQCRMLKKSGLVVEDHLFDTLPL
EVKEALNQDINIPFANGI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_018103
ORF Size 2574 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_018103.5
RefSeq Size 3782 bp
RefSeq ORF 2577 bp
Locus ID 55144
UniProt ID Q7L1W4
Cytogenetics 1p22.2
Domains LRR, LRR_TYP, LRR_BAC
Protein Families Transmembrane
MW 98.2 kDa
Gene Summary Non-essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes (PubMed:24790029, PubMed:26530471, PubMed:26824658, PubMed:28193731). The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine (PubMed:24790029, PubMed:26824658, PubMed:28193731). Plays a redundant role in the efflux of amino acids, such as aspartate, in response to osmotic stress (PubMed:28193731). Channel activity requires LRRC8A plus at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition (PubMed:24782309, PubMed:24790029, PubMed:26824658, PubMed:28193731). LRRC8A and LRRC8D are required for the uptake of the drug cisplatin (PubMed:26530471). Mediates the import of the antibiotic blasticidin-S into the cell (PubMed:24782309).[UniProtKB/Swiss-Prot Function]

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