LRRC8D (NM_018103) Human Tagged ORF Clone

CAT#: RC203641

LRRC8D (Myc-DDK-tagged)-Human leucine rich repeat containing 8 family, member D (LRRC8D), transcript variant 2

Product Images

Circular map for RC203641

• 

  Western blot validation of overexpression lysate (Cat# LY427455) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226214 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LRRC8D
• Synonyms: LRRC5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203641 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTACCCTTGCGGAAGTTGCATCACTTAATGACATTCAGCCAACTTACCGAATCCTGAAACCATGGT
  GGGATGTGTTTATGGATTACCTAGCTGTTGTTATGTTAATGGTAGCCATCTTTGCAGGAACCATGCAACT
  TACCAAAGATCAGGTGGTCTGTTTGCCAGTATTGCCATCTCCTGTAAATTCAAAGGCACATACACCACCA
  GGAAATGCCGAGGTCACCACCAACATCCCAAAGATGGAAGCAGCCACCAACCAAGACCAAGATGGGCGGA
  CAACAAACGACATTTCCTTTGGGACATCTGCTGTGACACCTGACATACCTCTCAGAGCCACATATCCTCG
  CACAGATTTCGCACTTCCAAATCAGGAGGCAAAGAAAGAGAAGAAAGATCCAACAGGTCGAAAAACAAAC
  TTGGATTTTCAGCAATATGTATTTATTAATCAAATGTGTTACCATCTGGCCCTTCCGTGGTATTCTAAGT
  ACTTTCCATACCTAGCTCTTATACATACTATTATTCTCATGGTCAGTAGCAACTTTTGGTTCAAATATCC
  CAAAACATGCTCAAAAGTAGAACATTTTGTTTCAATATTAGGAAAGTGCTTTGAATCCCCTTGGACGACA
  AAAGCGTTGTCTGAGACAGCATGCGAAGACTCAGAGGAAAACAAGCAGAGAATAACAGGTGCCCAGACTC
  TACCAAAGCATGTTTCTACCAGCAGTGATGAAGGGAGCCCCAGTGCCAGTACACCAATGATCAATAAAAC
  TGGCTTTAAATTTTCAGCTGAGAAGCCTGTGATTGAAGTTCCCAGCATGACAATCCTGGATAAAAAGGAT
  GGAGAGCAGGCCAAAGCCCTGTTTGAGAAAGTGAGGAAGTTCCGTGCCCATGTGGAAGATAGTGACTTGA
  TCTATAAACTCTATGTGGTCCAAACAGTTATCAAAACAGCCAAGTTCATTTTTATTCTCTGCTATACAGC
  GAACTTTGTCAACGCAATCAGCTTTGAACACGTCTGCAAGCCCAAAGTTGAGCATCTGATTGGTTATGAG
  GTATTTGAGTGCACCCACAATATGGCTTACATGTTGAAAAAGCTTCTCATCAGTTACATATCCATTATTT
  GTGTTTATGGCTTTATCTGCCTCTACACTCTCTTCTGGTTATTCAGGATACCTTTGAAGGAATATTCTTT
  CGAAAAAGTCAGAGAAGAGAGCAGTTTTAGTGACATTCCAGATGTCAAAAACGATTTTGCGTTCCTTCTT
  CACATGGTAGACCAGTATGACCAGCTATATTCCAAGCGTTTTGGTGTGTTCTTGTCAGAAGTTAGTGAAA
  ATAAACTTAGGGAAATTAGTTTGAACCATGAGTGGACATTTGAAAAACTCAGGCAGCACATTTCACGCAA
  CGCCCAGGACAAGCAGGAGTTGCATCTGTTCATGCTGTCGGGGGTGCCCGATGCTGTCTTTGACCTCACA
  GACCTGGATGTGCTAAAGCTTGAACTAATTCCAGAAGCTAAAATTCCTGCTAAGATTTCTCAAATGACTA
  ACCTCCAAGAGCTCCACCTCTGCCACTGCCCTGCAAAAGTTGAACAGACTGCTTTTAGCTTTCTTCGCGA
  TCACTTGAGATGCCTTCACGTGAAGTTCACTGATGTGGCTGAAATTCCTGCCTGGGTGTATTTGCTCAAA
  AACCTTCGAGAGTTGTACTTAATAGGCAATTTGAACTCTGAAAACAATAAGATGATAGGACTTGAATCTC
  TCCGAGAGTTGCGGCACCTTAAGATTCTCCACGTGAAGAGCAATTTGACCAAAGTTCCCTCCAACATTAC
  AGATGTGGCTCCACATCTTACAAAGTTAGTCATTCATAATGACGGCACTAAACTCTTGGTACTGAACAGC
  CTTAAGAAAATGATGAATGTCGCTGAGCTGGAACTCCAGAACTGTGAGCTAGAGAGAATCCCACATGCTA
  TTTTCAGCCTCTCTAATTTACAGGAACTGGATTTAAAGTCCAATAACATTCGCACAATTGAGGAAATCAT
  CAGTTTCCAGCATTTAAAACGACTGACTTGTTTAAAATTATGGCATAACAAAATTGTTACTATTCCTCCC
  TCTATTACCCATGTCAAAAACTTGGAGTCACTTTATTTCTCTAACAACAAGCTCGAATCCTTACCAGTGG
  CAGTATTTAGTTTACAGAAACTCAGATGCTTAGATGTGAGCTACAACAACATTTCAATGATTCCAATAGA
  AATAGGATTGCTTCAGAACCTGCAGCATTTGCATATCACTGGGAACAAAGTGGACATTCTGCCAAAACAA
  TTGTTTAAATGCATAAAGTTGAGGACTTTGAATCTGGGACAGAACTGCATCACCTCACTCCCAGAGAAAG
  TTGGTCAGCTCTCCCAGCTCACTCAGCTGGAGCTGAAGGGGAACTGCTTGGACCGCCTGCCAGCCCAGCT
  GGGCCAGTGTCGGATGCTCAAGAAAAGCGGGCTTGTTGTGGAAGATCACCTTTTTGATACCCTGCCACTC
  GAAGTCAAAGAGGCATTGAATCAAGACATAAATATTCCCTTTGCAAATGGGATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203641 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFTLAEVASLNDIQPTYRILKPWWDVFMDYLAVVMLMVAIFAGTMQLTKDQVVCLPVLPSPVNSKAHTPP
  GNAEVTTNIPKMEAATNQDQDGRTTNDISFGTSAVTPDIPLRATYPRTDFALPNQEAKKEKKDPTGRKTN
  LDFQQYVFINQMCYHLALPWYSKYFPYLALIHTIILMVSSNFWFKYPKTCSKVEHFVSILGKCFESPWTT
  KALSETACEDSEENKQRITGAQTLPKHVSTSSDEGSPSASTPMINKTGFKFSAEKPVIEVPSMTILDKKD
  GEQAKALFEKVRKFRAHVEDSDLIYKLYVVQTVIKTAKFIFILCYTANFVNAISFEHVCKPKVEHLIGYE
  VFECTHNMAYMLKKLLISYISIICVYGFICLYTLFWLFRIPLKEYSFEKVREESSFSDIPDVKNDFAFLL
  HMVDQYDQLYSKRFGVFLSEVSENKLREISLNHEWTFEKLRQHISRNAQDKQELHLFMLSGVPDAVFDLT
  DLDVLKLELIPEAKIPAKISQMTNLQELHLCHCPAKVEQTAFSFLRDHLRCLHVKFTDVAEIPAWVYLLK
  NLRELYLIGNLNSENNKMIGLESLRELRHLKILHVKSNLTKVPSNITDVAPHLTKLVIHNDGTKLLVLNS
  LKKMMNVAELELQNCELERIPHAIFSLSNLQELDLKSNNIRTIEEIISFQHLKRLTCLKLWHNKIVTIPP
  SITHVKNLESLYFSNNKLESLPVAVFSLQKLRCLDVSYNNISMIPIEIGLLQNLQHLHITGNKVDILPKQ
  LFKCIKLRTLNLGQNCITSLPEKVGQLSQLTQLELKGNCLDRLPAQLGQCRMLKKSGLVVEDHLFDTLPL
  EVKEALNQDINIPFANGI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018103
• ORF Size: 2574 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018103.5
• RefSeq Size: 3782 bp
• RefSeq ORF: 2577 bp
• Locus ID: 55144
• UniProt ID: Q7L1W4
• Cytogenetics: 1p22.2
• Domains: LRR, LRR_TYP, LRR_BAC
• Protein Families: Transmembrane
• MW: 98.2 kDa
• Gene Summary: Non-essential component of the volume-regulated anion channel (VRAC, also named VSOAC channel), an anion channel required to maintain a constant cell volume in response to extracellular or intracellular osmotic changes (PubMed:24790029, PubMed:26530471, PubMed:26824658, PubMed:28193731). The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine (PubMed:24790029, PubMed:26824658, PubMed:28193731). Plays a redundant role in the efflux of amino acids, such as aspartate, in response to osmotic stress (PubMed:28193731). Channel activity requires LRRC8A plus at least one other family member (LRRC8B, LRRC8C, LRRC8D or LRRC8E); channel characteristics depend on the precise subunit composition (PubMed:24782309, PubMed:24790029, PubMed:26824658, PubMed:28193731). LRRC8A and LRRC8D are required for the uptake of the drug cisplatin (PubMed:26530471). Mediates the import of the antibiotic blasticidin-S into the cell (PubMed:24782309).[UniProtKB/Swiss-Prot Function]