ZNF414 (NM_032370) Human Tagged ORF Clone

CAT#: RC203558

ZNF414 (Myc-DDK-tagged)-Human zinc finger protein 414 (ZNF414), transcript variant 2

Product Images

Circular map for RC203558

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF414
• Synonyms: ZFP414
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203558 representing NM_032370
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAGAAACCCTCAGGGCCCATCCCAGACATGCTGGCCACTGCAGAGCCCAGCTCCAGTGAGACCG
  ACAAGGAGGTGTTGTCCCCGGCTGTGCCAGCTGCAGCCCCTTCCTCCTCCATGTCGGAGGAGCCAGGCCC
  TGAGCAGGCAGCCACACCGCCAGTGTGGGAACGTGGAGGGGCTGGAGGGATGCAGCAGGGCTCCTCCCCA
  GCCCCAGACAGCTGCCAGCCTGGCCCCGGACCCAGCCCTGGCCTGACCAGCATAGTCTCCGGGACCAGCG
  AGGACCTGCGGCCTCCCAGACGACGCCCACCTCCAGGGAAGCAAATCCCTTGCTCCAGCCCTGGCTGCTG
  CCTCAGTTTTCCCAGCGTCCGTGACCTGGCACAGCATCTGCGAACCCACTGCCCGCCCACACAGTCCCTG
  GAAGGCAAGCTCTTCCGCTGCTCAGCCCTGAGCTGCACCGAGACCTTCCCCAGCATGCAGGAGCTGGTGG
  CTCACAGCAAACTGCACTACAAACCCAATCGCTACTTCAAGTGTGAGAACTGCCTCCTGCGCTTCCGCAC
  GCACCGCTCGCTCTTCAAGCACCTGCATGTTTGCGCGGAGCATGCGCAGAGCCCAGCCCCGCCGCCACCC
  CCGGCCCTGGACCGAGAGCCGCCCGCGCCGGAGCGCCCCCCGGAGGTTGACCCCGCATCAGCGCCGGGCC
  TGCCGTTCCCGCTGCTGGAACCCTTCACGACCCCCGCCCCTGCCCCCACCGGACCGTTCCTGCCCTACTT
  GAACCCTGCGCCCTTTGGCCTAAGCCCCCCGCGCCTGCGCCCCTTCCTGGCCGCTGCACCCGGGCCGCCG
  GCTTCCAGCGCCGCCGTCTGGAAAAAGAGCCAAGGTGCTGGCAGCAGCCCCCGAAGACCCCAGGGCGGCT
  CCGACGCGCCCTCAGGTGCGTGCAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203558 representing NM_032370
  Red=Cloning site Green=Tags(s)
  
  MEEKPSGPIPDMLATAEPSSSETDKEVLSPAVPAAAPSSSMSEEPGPEQAATPPVWERGGAGGMQQGSSP
  APDSCQPGPGPSPGLTSIVSGTSEDLRPPRRRPPPGKQIPCSSPGCCLSFPSVRDLAQHLRTHCPPTQSL
  EGKLFRCSALSCTETFPSMQELVAHSKLHYKPNRYFKCENCLLRFRTHRSLFKHLHVCAEHAQSPAPPPP
  PALDREPPAPERPPEVDPASAPGLPFPLLEPFTTPAPAPTGPFLPYLNPAPFGLSPPRLRPFLAAAPGPP
  ASSAAVWKKSQGAGSSPRRPQGGSDAPSGACR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032370
• ORF Size: 936 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032370.3
• RefSeq Size: 1204 bp
• RefSeq ORF: 939 bp
• Locus ID: 84330
• UniProt ID: Q96IQ9
• Cytogenetics: 19p13.2
• MW: 32.8 kDa
• Gene Summary: May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]