SOX10 (NM_006941) Human Tagged ORF Clone

CAT#: RC203545

SOX10 (Myc-DDK-tagged)-Human SRY (sex determining region Y)-box 10 (SOX10)

Product Images

Circular map for RC203545

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  Western blot validation of overexpression lysate (Cat# LY402065) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203545 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SOX10 protein (Cat# TP303545). The protein was produced from HEK293T cells transfected with SOX10 cDNA clone (Cat# RC203545) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SOX10
• Synonyms: DOM; PCWH; WS2E; WS4; WS4C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203545 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGGAGCAGGACCTATCGGAGGTGGAGCTGAGCCCCGTGGGCTCGGAGGAGCCCCGCTGCCTGT
  CCCCGGGGAGCGCGCCCTCGCTAGGGCCCGACGGCGGCGGCGGCGGATCGGGCCTGCGAGCCAGCCCGGG
  GCCAGGCGAGCTGGGCAAGGTCAAGAAGGAGCAGCAGGACGGCGAGGCGGACGATGACAAGTTCCCCGTG
  TGCATCCGCGAGGCCGTCAGCCAGGTGCTCAGCGGCTACGACTGGACGCTGGTGCCCATGCCCGTGCGCG
  TCAACGGCGCCAGCAAAAGCAAGCCGCACGTCAAGCGGCCCATGAACGCCTTCATGGTGTGGGCTCAGGC
  AGCGCGCAGGAAGCTCGCGGACCAGTACCCGCACCTGCACAACGCTGAGCTCAGCAAGACGCTGGGCAAG
  CTCTGGAGGCTGCTGAACGAAAGTGACAAGCGCCCCTTCATCGAGGAGGCTGAGCGGCTCCGTATGCAGC
  ACAAGAAAGACCACCCGGACTACAAGTACCAGCCCAGGCGGCGGAAGAACGGGAAGGCCGCCCAGGGCGA
  GGCGGAGTGCCCCGGTGGGGAGGCCGAGCAAGGTGGGACCGCCGCCATCCAGGCCCACTACAAGAGCGCC
  CACTTGGACCACCGGCACCCAGGAGAGGGCTCCCCCATGTCAGATGGTAACCCCGAGCACCCCTCAGGCC
  AGAGCCATGGCCCACCCACCCCTCCAACCACCCCGAAGACAGAGCTGCAGTCGGGCAAGGCAGACCCGAA
  GCGGGACGGGCGCTCCATGGGGGAGGGCGGGAAGCCTCACATCGACTTCGGCAACGTGGACATTGGTGAG
  ATCAGCCACGAGGTAATGTCCAACATGGAGACCTTTGATGTGGCTGAGTTGGACCAGTACCTGCCGCCCA
  ATGGGCACCCAGGCCATGTGAGCAGCTACTCAGCAGCCGGCTATGGGCTGGGCAGTGCCCTGGCCGTGGC
  CAGTGGACACTCCGCCTGGATCTCCAAGCCACCAGGCGTGGCTCTGCCCACGGTCTCACCACCTGGTGTG
  GATGCCAAAGCCCAGGTGAAGACAGAGACCGCGGGGCCCCAGGGGCCCCCACACTACACCGACCAGCCAT
  CCACCTCACAGATCGCCTACACCTCCCTCAGCCTGCCCCACTATGGCTCAGCCTTCCCCTCCATCTCCCG
  CCCCCAGTTTGACTACTCTGACCATCAGCCCTCAGGACCCTATTATGGCCACTCGGGCCAGGCCTCTGGC
  CTCTACTCGGCCTTCTCCTATATGGGGCCCTCGCAGCGGCCCCTCTACACGGCCATCTCTGACCCCAGCC
  CCTCAGGGCCCCAGTCCCACAGCCCCACACACTGGGAGCAGCCAGTATATACGACACTGTCCCGGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203545 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAEEQDLSEVELSPVGSEEPRCLSPGSAPSLGPDGGGGGSGLRASPGPGELGKVKKEQQDGEADDDKFPV
  CIREAVSQVLSGYDWTLVPMPVRVNGASKSKPHVKRPMNAFMVWAQAARRKLADQYPHLHNAELSKTLGK
  LWRLLNESDKRPFIEEAERLRMQHKKDHPDYKYQPRRRKNGKAAQGEAECPGGEAEQGGTAAIQAHYKSA
  HLDHRHPGEGSPMSDGNPEHPSGQSHGPPTPPTTPKTELQSGKADPKRDGRSMGEGGKPHIDFGNVDIGE
  ISHEVMSNMETFDVAELDQYLPPNGHPGHVSSYSAAGYGLGSALAVASGHSAWISKPPGVALPTVSPPGV
  DAKAQVKTETAGPQGPPHYTDQPSTSQIAYTSLSLPHYGSAFPSISRPQFDYSDHQPSGPYYGHSGQASG
  LYSAFSYMGPSQRPLYTAISDPSPSGPQSHSPTHWEQPVYTTLSRP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006941
• ORF Size: 1398 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006941.2
• RefSeq Size: 2882 bp
• RefSeq ORF: 1401 bp
• Locus ID: 6663
• UniProt ID: P56693
• Cytogenetics: 22q13.1
• Protein Families: Adult stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors
• MW: 49.9 kDa
• Gene Summary: This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease. [provided by RefSeq, Jul 2008]