RPP25 (NM_017793) Human Tagged ORF Clone

CAT#: RC203538

RPP25 (Myc-DDK-tagged)-Human ribonuclease P/MRP 25kDa subunit (RPP25)

Product Images

Circular map for RC203538

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  Western blot validation of overexpression lysate (Cat# LY413534) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203538 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified RPP25 protein (Cat# TP303538). The protein was produced from HEK293T cells transfected with RPP25 cDNA clone (Cat# RC203538) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RPP25
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203538 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGGAGAACTTCCGTAAGGTGCGCTCCGAAGAGGCGCCAGCGGGGTGCGGGGCCGAGGGAGGCGGCCCGG
  GCTCCGGCCCCTTCGCAGACCTGGCGCCGGGCGCGGTGCACATGCGGGTCAAGGAAGGCAGCAAGATCCG
  GAACCTGATGGCCTTCGCCACCGCCAGCATGGCGCAGCCAGCCACGCGCGCCATCGTCTTCAGCGGCTGC
  GGCCGGGCCACCACCAAAACCGTCACGTGCGCCGAGATCCTCAAGCGCCGCCTGGCGGGCCTGCACCAGG
  TCACGCGGCTGCGCTACCGGAGCGTACGCGAGGTGTGGCAGAGCCTCCCGCCTGGGCCCACGCAGGGTCA
  GACGCCTGGCGAGCCGGCCGCTAGTCTCAGCGTACTTAAGAACGTGCCCGGCCTCGCCATCCTACTTTCC
  AAGGACGCGCTGGATCCGCGACAGCCCGGCTACCAGCCCCCGAATCCCCATCCTGGTCCCTCGTCCCCGC
  CAGCCGCGCCAGCGTCCAAGAGGAGCCTAGGGGAACCCGCAGCTGGAGAAGGCTCCGCGAAGCGATCGCA
  ACCCGAGCCAGGGGTTGCGGACGAGGATCAGACGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203538 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MENFRKVRSEEAPAGCGAEGGGPGSGPFADLAPGAVHMRVKEGSKIRNLMAFATASMAQPATRAIVFSGC
  GRATTKTVTCAEILKRRLAGLHQVTRLRYRSVREVWQSLPPGPTQGQTPGEPAASLSVLKNVPGLAILLS
  KDALDPRQPGYQPPNPHPGPSSPPAAPASKRSLGEPAAGEGSAKRSQPEPGVADEDQTA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017793
• ORF Size: 597 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017793.3
• RefSeq Size: 2333 bp
• RefSeq ORF: 600 bp
• Locus ID: 54913
• UniProt ID: Q9BUL9
• Cytogenetics: 15q24.2
• MW: 20.6 kDa
• Gene Summary: Component of ribonuclease P, a ribonucleoprotein complex that generates mature tRNA molecules by cleaving their 5'-ends (PubMed:12003489, PubMed:16723659, PubMed:30454648). Also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences (PubMed:28115465).[UniProtKB/Swiss-Prot Function]