RPP25 (NM_017793) Human Tagged ORF Clone

CAT#: RC203538

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RPP25 (Myc-DDK-tagged)-Human ribonuclease P/MRP 25kDa subunit (RPP25)



  "NM_017793" in other vectors (4)

Reconstitution Protocol

USD 300.00

USD 450.00

In Stock*

Size
    • 10 ug

Product images

Frequently bought together (4)
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol RPP25
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC203538 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
GCC
C

ATGGAGAACTTCCGTAAGGTGCGCTCCGAAGAGGCGCCAGCGGGGTGCGGGGCCGAGGGAGGCGGCCCGG
GCTCCGGCCCCTTCGCAGACCTGGCGCCGGGCGCGGTGCACATGCGGGTCAAGGAAGGCAGCAAGATCCG
GAACCTGATGGCCTTCGCCACCGCCAGCATGGCGCAGCCAGCCACGCGCGCCATCGTCTTCAGCGGCTGC
GGCCGGGCCACCACCAAAACCGTCACGTGCGCCGAGATCCTCAAGCGCCGCCTGGCGGGCCTGCACCAGG
TCACGCGGCTGCGCTACCGGAGCGTACGCGAGGTGTGGCAGAGCCTCCCGCCTGGGCCCACGCAGGGTCA
GACGCCTGGCGAGCCGGCCGCTAGTCTCAGCGTACTTAAGAACGTGCCCGGCCTCGCCATCCTACTTTCC
AAGGACGCGCTGGATCCGCGACAGCCCGGCTACCAGCCCCCGAATCCCCATCCTGGTCCCTCGTCCCCGC
CAGCCGCGCCAGCGTCCAAGAGGAGCCTAGGGGAACCCGCAGCTGGAGAAGGCTCCGCGAAGCGATCGCA
ACCCGAGCCAGGGGTTGCGGACGAGGATCAGACGGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC203538 protein sequence
Red=Cloning site Green=Tags(s)

MENFRKVRSEEAPAGCGAEGGGPGSGPFADLAPGAVHMRVKEGSKIRNLMAFATASMAQPATRAIVFSGC
GRATTKTVTCAEILKRRLAGLHQVTRLRYRSVREVWQSLPPGPTQGQTPGEPAASLSVLKNVPGLAILLS
KDALDPRQPGYQPPNPHPGPSSPPAAPASKRSLGEPAAGEGSAKRSQPEPGVADEDQTA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites AscI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_017793
ORF Size 597 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_017793.3
RefSeq Size 2333 bp
RefSeq ORF 600 bp
Locus ID 54913
UniProt ID Q9BUL9
Cytogenetics 15q24.2
MW 20.6 kDa
Gene Summary Component of ribonuclease P, a ribonucleoprotein complex that generates mature tRNA molecules by cleaving their 5'-ends (PubMed:12003489, PubMed:16723659, PubMed:30454648). Also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences (PubMed:28115465).[UniProtKB/Swiss-Prot Function]

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