ZNRF1 (NM_032268) Human Tagged ORF Clone

CAT#: RC203521

ZNRF1 (Myc-DDK-tagged)-Human zinc and ring finger 1 (ZNRF1)

Product Images

Circular map for RC203521

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  Western blot validation of overexpression lysate (Cat# LY410254) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203521 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNRF1
• Synonyms: NIN283
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203521 representing NM_032268
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGGCAAGCAGAGCACGGCGGCCCGCTCCCGGGGCCCCTTCCCGGGGGTCTCCACCGATGACAGCG
  CCGTGCCGCCGCCGGGAGGGGCGCCCCATTTCGGGCACTACCGGACGGGCGGCGGGGCCATGGGGCTGCG
  CAGCCGCTCGGTCAGCTCGGTGGCAGGCATGGGCATGGACCCCAGCACGGCCGGGGGGGTGCCCTTTGGC
  CTCTACACCCCCGCCTCCCGGGGCACCGGCGACTCCGAGAGGGCGCCCGGCGGCGGAGGGTCTGCGTCCG
  ACTCCACCTATGCCCATGGCAATGGTTACCAGGAGACGGGCGGCGGTCACCATAGAGACGGGATGCTGTA
  CCTGGGCTCCCGAGCCTCGCTGGCGGATGCTCTACCTCTGCACATCGCACCCAGGTGGTTCAGCTCGCAT
  AGTGGTTTCAAGTGCCCCATTTGCTCCAAGTCTGTGGCTTCTGACGAGATGGAAATGCACTTTATAATGT
  GTTTGAGCAAACCTCGCCTCTCCTACAACGATGATGTGCTGACTAAAGACGCGGGTGAGTGTGTGATCTG
  CCTGGAGGAGCTGCTGCAGGGGGACACGATAGCCAGGCTGCCCTGCCTGTGCATCTATCACAAAAGCTGC
  ATAGACTCGTGGTTTGAAGTGAACAGATCTTGTCCGGAACACCCTGCGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203521 representing NM_032268
  Red=Cloning site Green=Tags(s)
  
  MGGKQSTAARSRGPFPGVSTDDSAVPPPGGAPHFGHYRTGGGAMGLRSRSVSSVAGMGMDPSTAGGVPFG
  LYTPASRGTGDSERAPGGGGSASDSTYAHGNGYQETGGGHHRDGMLYLGSRASLADALPLHIAPRWFSSH
  SGFKCPICSKSVASDEMEMHFIMCLSKPRLSYNDDVLTKDAGECVICLEELLQGDTIARLPCLCIYHKSC
  IDSWFEVNRSCPEHPAD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032268
• ORF Size: 681 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032268.5
• RefSeq Size: 4633 bp
• RefSeq ORF: 684 bp
• Locus ID: 84937
• UniProt ID: Q8ND25
• Cytogenetics: 16q23.1
• Domains: RING
• Protein Families: Druggable Genome
• MW: 23.8 kDa
• Gene Summary: This gene encodes an E3 ubiquitin-protein ligase that plays a role in neural-cell differentiation. Overexpression of this gene causes neurite-like elongation. The encoded protein contains both a zinc finger and a RING finger motif and is localized in the endosome/lysosome compartment, indicating that it may be involved in ubiquitin-mediated protein modification, and in synaptic vessicle membranes in neurons. [provided by RefSeq, Feb 2012]